Trpm3 (NM_001035239) Mouse Tagged ORF Clone

CAT#: MG224626

Trpm3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 3 (Trpm3) transcript variant 1, (10ug)

Product Images

Circular map for MG224626

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm3
• Synonyms: 6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224626 representing NM_001035239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA
  GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA
  GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA
  TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC
  TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT
  CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT
  TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA
  CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC
  CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC
  AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG
  GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG
  AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT
  ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA
  AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG
  AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG
  GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC
  TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT
  GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC
  ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA
  TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA
  CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA
  GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA
  TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT
  GTACCACTTGGTCAGGGATGTCAAAAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATT
  GGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCC
  TCTACCACAATCTCTTTGGCCCCAAAAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCC
  CTTGAGGAGAGGACGGAAGACAACTAAGAAGCGTGAGGAAGAGGTGGACATCGACTTGGATGATCCTGAG
  ATCAACCACTTTCCATTCCCCTTCCATGAGCTGATGGTGTGGGCCGTCCTCATGAAGAGACAAAAGATGG
  CCCTGTTCTTCTGGCAACACGGAGAGGAGGCCATGGCCAAAGCCCTGGTGGCCTGCAAGCTCTGCAAGGC
  CATGGCTCACGAGGCTTCTGAGAATGACATGGTCGATGACATTTCCCAGGAGCTGAACCACAACTCCAGG
  GACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAACAGGATGAGCAGCTGGCCATGAAGC
  TGCTGACGTATGAGCTGAAGAACTGGAGTAATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCG
  TGACTTCATTGCACACACGTGCAGCCAGATGTTACTTACTGACATGTGGATGGGACGGCTCCGGATGAGA
  AAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGA
  ACAAAGATGACATGCCCTATATGACTCAGGCCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGAGGAGCC
  AGAGAAGCCCACAAAGGAAAAAGATGAAGAGGACATGGAGCTAACAGCAATGTTGGGGCGAAGCAATGGG
  GAATCATCCAGAAAGAAAGATGAAGAAGAAGTTCAAAGCAGGCACCGGCTAATCCCCGTGGGCCGAAAAA
  TCTATGAGTTCTACAACGCACCCATCGTGAAGTTCTGGTTCTACACTCTGGCGTATATTGGATACCTGAT
  GCTCTTCAACTACATCGTGTTAGTGAAGATGGAGCGCTGGCCTTCCACTCAGGAATGGATTGTCATCTCC
  TACATTTTCACTCTGGGAATAGAGAAGATGAGAGAGATCCTGATGTCGGAGCCGGGCAAGCTGCTGCAGA
  AGGTGAAGGTGTGGCTTCAGGAGTACTGGAACGTCACAGACCTCATCGCCATCCTTCTCTTCTCGGTGGG
  AATGATCCTTCGTCTTCAAGATCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGTGTGAACATCATT
  TATTGGTATATCCGTTTGCTGGACATCTTCGGCGTGAACAAGTATCTGGGCCCATATGTGATGATGATTG
  GGAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGC
  CAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCATCTTGGAAACTGGCCAAGAATATCTTCTACATGCCT
  TATTGGATGATTTATGGGGAAGTGTTTGCTGACCAGATAGACCGTAAGCAAGTTTATGATTCTCATACAC
  CAAAGTCAGCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCATGCAA
  GACAGGAGCATGGATTGTCCCGGCCATCATGGCCTGCTACCTCTTGGTGGCGAACATCCTTCTGGTCAAC
  CTCCTTATTGCCGTCTTCAACAATACATTTTTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAATTTC
  AGAGGTACCAGCTCATCATGACTTTCCACGAGAGGCCGGTTCTGCCCCCACCGCTCATCATCTTCAGTCA
  CATGACCATGATCTTCCAGCATGTGTGCTGCCGGTGGAGGAAGCATGAGAGTGACCAGGACGAAAGGGAC
  TACGGCCTGAAACTCTTCATAACTGACGATGAGCTCAAGAAAGTACACGATTTTGAAGAGCAGTGCATAG
  AGGAATATTTCCGAGAGAAGGATGATCGCTTCAATTCGTCCAACGATGAGAGGATACGTGTTACATCAGA
  AAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGTTAATGAGAGAGAACATTCCATGAAGGCTTCACTC
  CAGACCGTGGACATCCGGCTAGCACAGCTAGAGGACCTCATCGGGCGCATGGCCACCGCCCTGGAGCGCC
  TGACTGGTCTGGAGCGGGCAGAGTCTAACAAAATCCGCTCAAGGACCTCCTCAGACTGCACAGATGCAGC
  CTACATCGTCCGCCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTATAGAC
  CCTGCAGGTGAGGAGACCATATCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCT
  ATTCAGTCAATGTGAAAGACAAAGGTGGGATAGAAAAGTTGGAAAGCATTTTCAAAGAAAGGTCCCTGAG
  CTTACACCGAGCTACTAGCTCCCACTCAGTAGCCAAAGAACCCAAAGCTCCTGCAGCCCCTGCAAACACC
  TTGGCCATTGTTCCTGACTCCAGAAGACCATCATCTTGCATAGACATCTATGTCTCTGCCATGGACGAGC
  TCCACTGTGATATAGAGCCTCTGGATAATTCCATGAACATCCTTGGGCTGGGTGAGCCAAGCTTTTCAGC
  TCTAGCACCTTCCACAACCCCGTCAAGTAGTGCCTATGCAACGCTCGCACCTACAGACCGACCTCCCAGT
  AGGAGCATTGATTTTGAAGACCTCACCTCCATGGACACTAGATCTTTTTCTTCAGACTATACACACCTCC
  CAGAATGCCAAAACCCCTGGGACACAGACCCTCCAACGTACCATACCATCGAGCGTTCCAAGAGTAGCCG
  CTACCTAGCCACCACGCCCTTTCTTCTGGAAGAGGCTCCCATTGTAAAATCCCATAGCTTTATGTTTTCT
  CCTTCAAGGAGCTACTACGCCAACTTTGGGGTGCCCGTGAAAACGGCAGAATACACAAGTATTACAGACT
  GTATCGACACAAGATGTGTCAATGCCCCCCAAGCAATAGCTGACCGAGCCACCTTCCCTGGAGGTCTCGG
  AGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGAGCGAGAAGCAGAGCTGAGCCATCCTAGCTCTGAC
  AGTGAGGAAAATGAGGCCAGAGGCCAGAGAGCTGCCAATCCGATATCCTCTCAGGAGGCTGAAAATGCAG
  ACAGAACCCTATCCAACAACATCACAGTTCCCAAGATAGAGCGCGCCAACAGCTACTCAGCAGAGGAGCC
  AAACGTGCCATATGCACATACCAGAAAGAGCTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAACACG
  GCGAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTATCCA
  TGAGGAGACTGTCTAGAACATCGGCCTTTCATAGCTTTGAAAGCAAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224626 representing NM_001035239
  Red=Cloning site Green=Tags(s)
  
  MGKKWRDAGELERGCSDREDSAESRRRSRSASRGRFAESWKRLSSKQGSTKRSGLPAQQTPAQKSWIERA
  FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPSISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDA
  FGTIEFQGGGHSNKAMYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLI
  KAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNP
  MSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVL
  EYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQHLFIILMEC
  MKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQ
  AMLDALVLDRVDFVKLLIENGVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDI
  GLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDIPLRRGRKTTKKREEEVDIDLDDPE
  INHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHNSR
  DFGQLAVELLDQSYKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMR
  KNSGLKVILGILLPPSILSLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRSNG
  ESSRKKDEEEVQSRHRLIPVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIVIS
  YIFTLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVNII
  YWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFYMP
  YWMIYGEVFADQIDRKQVYDSHTPKSAPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVN
  LLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHMTMIFQHVCCRWRKHESDQDERD
  YGLKLFITDDELKKVHDFEEQCIEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASL
  QTVDIRLAQLEDLIGRMATALERLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKLQESID
  PAGEETISPTSPTLMPRMRSHSFYSVNVKDKGGIEKLESIFKERSLSLHRATSSHSVAKEPKAPAAPANT
  LAIVPDSRRPSSCIDIYVSAMDELHCDIEPLDNSMNILGLGEPSFSALAPSTTPSSSAYATLAPTDRPPS
  RSIDFEDLTSMDTRSFSSDYTHLPECQNPWDTDPPTYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFS
  PSRSYYANFGVPVKTAEYTSITDCIDTRCVNAPQAIADRATFPGGLGDKVEDLSCCHPEREAELSHPSSD
  SEENEARGQRAANPISSQEAENADRTLSNNITVPKIERANSYSAEEPNVPYAHTRKSFSISDKLDRQRNT
  ASLRNPFQRSKSSKPEGRGDSLSMRRLSRTSAFHSFESKHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035239
• ORF Size: 5163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035239.3
• RefSeq Size: 6742 bp
• RefSeq ORF: 5166 bp
• Locus ID: 226025
• Cytogenetics: 19 B