Lsr (NM_001164184) Mouse Tagged ORF Clone

CAT#: MG224616

Lsr (tGFP-tagged) - Mouse lipolysis stimulated lipoprotein receptor (Lsr) transcript variant 2, (10ug)

Product Images

Circular map for MG224616

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lsr
• Synonyms: ILDR3; Lisch7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224616 representing NM_001164184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGCGGCCAGCGCGTGTGCTGGGGCGCCTGGCTCCCACCCGGCCACCACGATCTTCGTGTGTC
  TTTTTCTCATCATTTACTGCCCAGACCGTGCCAGTGCCATCCAGGTGACCGTGCCTGACCCCTACCACGT
  AGTGATCCTGTTCCAGCCAGTGACACTACACTGCACCTACCAGATGAGCAATACCCTCACAGCCCCTATC
  GTGATCTGGAAGTATAAGTCGTTCTGTCGGGACCGTGTTGCCGACGCCTTCTCCCCTGCCAGCGTGGACA
  ACCAGCTCAACGCCCAGCTGGCGGCTGGCAACCCCGGCTACAACCCCTATGTGGAGTGCCAGGACAGCGT
  ACGCACTGTCAGGGTGGTGGCCACCAAACAGGGCAATGCTGTGACCCTGGGAGACTACTACCAGGGCAGG
  AGAATCACCATCACAGGAAATGCTGACCTGACCTTCGAGCAGACGGCCTGGGGAGACAGTGGAGTGTATT
  ACTGCTCCGTGGTCTCAGCCCAAGATCTGGATGGGAACAACGAGGCGTACGCAGAGCTCATTGTCCTTGA
  TTGGCTCTTTGTGGTCGTGGTCTGCCTGGCAAGCCTCCTCTTCTTCCTCCTCCTGGGCATCTGCTGGTGC
  CAGTGCTGTCCCCACACCTGCTGCTGCTATGTCAGATGTCCCTGCTGCCCAGACAAGTGCTGTTGCCCTG
  AGGCCCTTTATGCTGCTGGCAAAGCAGCCACCTCAGGTGTGCCAAGCATCTATGCCCCCAGCATCTATAC
  CCACCTCTCTCCTGCCAAGACTCCGCCACCTCCGCCTGCCATGATTCCCATGCGTCCTCCCTATGGGTAC
  CCTGGAGACTTTGACAGGACCAGCTCAGTTGGTGGCCACAGCTCCCAGGTGCCCCTGCTGCGTGAAGTGG
  ATGGGAGCGTATCTTCAGAAGTACGAAGTGGCTACAGGATCCAGGCTAACCAGCAAGATGACTCCATGAG
  GGTCCTATACTATATGGAGAAGGAGCTAGCCAACTTCGATCCTTCCCGGCCTGGCCCTCCCAATGGCCGA
  GTGGAACGGGCCATGAGTGAAGTAACCTCCCTCCATGAAGATGACTGGCGATCTCGGCCTTCCAGGGCTC
  CTGCCCTCACACCCATCAGGGATGAGGAGTGGAATCGCCACTCCCCTCGGAGTCCCAGAACATGGGAGCA
  GGAACCCCTTCAAGAACAGCCAAGGGGTGGTTGGGGGTCTGGGCGGCCTCGGGCCCGCTCTGTGGATGCT
  CTAGATGACATCAACCGGCCTGGCTCCACTGAATCAGGAAGGTCTTCTCCCCCAAGTAGTGGACGGAGAG
  GGCGGGCCTATGCACCTCCGAGAAGTCGCAGCCGGGATGACCTCTATGACCCCGACGATCCTAGAGACTT
  GCCACATTCCCGAGATCCCCACTATTATGATGATTTGAGGTCTAGGGATCCACGTGCTGACCCCAGATCC
  CGTCAGCGATCCCACGATCCTCGGGATGCTGGCTTCAGGTCACGGGACCCTCAGTATGATGGGCGACTCT
  TAGAAGAGGCTTTAAAGAAAAAAGGGGCTGGGGAGAGAAGACGCGTTTACAGGGAGGAAGAAGAAGAAGA
  AGAGGAGGGCCACTATCCCCCAGCACCTCCGCCTTACTCTGAGACTGACTCGCAGGCCTCGAGGGAGCGG
  AGGATGAAAAAGAATTTGGCCCTGAGTCGGGAAAGTTTAGTCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224616 representing NM_001164184
  Red=Cloning site Green=Tags(s)
  
  MAPAASACAGAPGSHPATTIFVCLFLIIYCPDRASAIQVTVPDPYHVVILFQPVTLHCTYQMSNTLTAPI
  VIWKYKSFCRDRVADAFSPASVDNQLNAQLAAGNPGYNPYVECQDSVRTVRVVATKQGNAVTLGDYYQGR
  RITITGNADLTFEQTAWGDSGVYYCSVVSAQDLDGNNEAYAELIVLDWLFVVVVCLASLLFFLLLGICWC
  QCCPHTCCCYVRCPCCPDKCCCPEALYAAGKAATSGVPSIYAPSIYTHLSPAKTPPPPPAMIPMRPPYGY
  PGDFDRTSSVGGHSSQVPLLREVDGSVSSEVRSGYRIQANQQDDSMRVLYYMEKELANFDPSRPGPPNGR
  VERAMSEVTSLHEDDWRSRPSRAPALTPIRDEEWNRHSPRSPRTWEQEPLQEQPRGGWGSGRPRARSVDA
  LDDINRPGSTESGRSSPPSSGRRGRAYAPPRSRSRDDLYDPDDPRDLPHSRDPHYYDDLRSRDPRADPRS
  RQRSHDPRDAGFRSRDPQYDGRLLEEALKKKGAGERRRVYREEEEEEEEGHYPPAPPPYSETDSQASRER
  RMKKNLALSRESLVV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164184
• ORF Size: 1725 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164184.1, NP_001157656.1
• RefSeq Size: 2120 bp
• RefSeq ORF: 1728 bp
• Locus ID: 54135
• UniProt ID: Q99KG5
• Cytogenetics: 7 B1
• Gene Summary: Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity).[UniProtKB/Swiss-Prot Function]