Kcnj15 (NM_001039057) Mouse Tagged ORF Clone

CAT#: MG224600

Kcnj15 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel subfamily J member 15 (Kcnj15) transcript variant 1, (10ug)

Product Images

Circular map for MG224600

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnj15
• Synonyms: 4930414N08Rik; AI182284; AI267127; IRKK; Kir4.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224600 representing NM_001039057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGCCAGGTGGGAGAAGGGGAGCGAGGACGCGCCACTTACCCTGCAGAAGATTCCTGACCTACAGA
  GTGGTCCAAAGATGGATGCCATTCACCTTGGCATGTCCAGTGCCCCACTGGTGAAGCATACCAACGGGGT
  TGGACTCAAGGCCCACAGACCCCGAGTCATGTCAAAGAGTGGGCACAGTAATGTGAGAATCGATAAGGTA
  GACGGAATCTATTTACTCTACCTCCAGGACTTGTGGACAACCGTCATCGACATGAAGTGGCGATACAAGC
  TCACCCTATTTGCTGCCACCTTTGTGATGACCTGGTTTCTGTTTGGAGTGGTCTACTATGCCATAGCCTT
  TATTCATGGTGACTTACAACTTGGGGAATCTAATTCCAACCACACACCCTGCATTATGAAAGTGGACTCT
  CTCACAGGAGCATTCCTCTTTTCCTTGGAATCTCAGACAACCATTGGCTACGGGGTCCGTTCCATCACAG
  AGGAGTGTCCCCATGCTATCTTCCTCTTAGTCGCCCAACTGGTCATCACCACATTGATTGAGATCTTCAT
  TACGGGGACCTTTCTGGCTAAAATTGCAAGACCCAAAAAGCGAGCCGAGACCATTAAGTTCAGCCACTGT
  GCTGTCATCAGCAAGCAGAATGGAAAGCTATGCCTGGTCATCCAGGTGGCCAACATGAGGAAGAGTCTCC
  TGATTCAGTGCCAGCTCTCTGGAAAACTCCTGCAGACACACGTCACCAAAGAGGGAGAACGCATTCTCCT
  CAACCAGGCCACTGTCAAATTCCACGTGGACTCCTCTTCCGAGAGTCCCTTCCTCATCCTGCCCATGACC
  TTCTACCACGTGTTGGATGAGACAAGCCCCCTGCGGGACCTCACACCCCAAAACCTAAAGGAGAAGGAGT
  TTGAGCTGGTGGTACTTCTCAACGCCACGGTGGAGTCTACCAGCGCCGTCTGCCAGAGCCGAACGTCTTA
  CATCCCGGAGGAGATCTACTGGGGCTTTGAGTTTGTGCCTGTGGTTTCTCTCTCCAAAAATGGAAAGTAT
  GTGGCTGATTTCAGTCAATTTGAGCAGATCAGGAAGAGCCCGGATTGTACCTTCTACTGTGCCGATTCTG
  AGAAGCAGAAGCTTGAAGAACAGTACAGGCAAGAGGACCAGAGGGAGCGGGAGCTGAGGAGCCTCCTGCT
  ACAGCAGAGCAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224600 representing NM_001039057
  Red=Cloning site Green=Tags(s)
  
  MVARWEKGSEDAPLTLQKIPDLQSGPKMDAIHLGMSSAPLVKHTNGVGLKAHRPRVMSKSGHSNVRIDKV
  DGIYLLYLQDLWTTVIDMKWRYKLTLFAATFVMTWFLFGVVYYAIAFIHGDLQLGESNSNHTPCIMKVDS
  LTGAFLFSLESQTTIGYGVRSITEECPHAIFLLVAQLVITTLIEIFITGTFLAKIARPKKRAETIKFSHC
  AVISKQNGKLCLVIQVANMRKSLLIQCQLSGKLLQTHVTKEGERILLNQATVKFHVDSSSESPFLILPMT
  FYHVLDETSPLRDLTPQNLKEKEFELVVLLNATVESTSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKY
  VADFSQFEQIRKSPDCTFYCADSEKQKLEEQYRQEDQRERELRSLLLQQSNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039057
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039057.2
• RefSeq Size: 5138 bp
• RefSeq ORF: 1209 bp
• Locus ID: 16516
• Cytogenetics: 16 55.86 cM
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]