Ppp3cc (NM_008915) Mouse Tagged ORF Clone

CAT#: MG224578

Ppp3cc (tGFP-tagged) - Mouse protein phosphatase 3 catalytic subunit gamma isoform (Ppp3cc), (10ug)

Product Images

Circular map for MG224578

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp3cc
• Synonyms: Calnc; PP2BA gamma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224578 representing NM_008915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTGAGGCGCCCTCAGTTCTCCACGACCGAGCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCC
  GACGGCTAACTTTGAAGGAAGTTTTTGAGAATGGGAAACCTAAAATGGATCTTTTGAAAAACCATTTAGT
  AAAAGAAGGTCGGGTGGAAGAGGAGGTGGCCTTAAAGATCATCAATGATGGGGCTGCCATCCTGAAGCAG
  GAGAAGACCATGATAGAGGTGGAGGCTCCGATCACAGTGTGTGGTGATGTTCATGGACAATTCTTTGACC
  TGATGAAGTTGTTTGAAGTTGGGGGATCACCTAGTAATACTCGCTACCTCTTCCTGGGTGACTATGTGGA
  CAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATCTATGGAGCTTAAAGATTAACCATCCTAAAACATTG
  TTTCTGCTTCGAGGAAATCATGAATGCAGGCATCTTACAGAGTACTTCACCTTCAAACAGGAATGTCGGA
  TCAAGTATTCAGAGATGGTGTACGATGCGTGCATGCACACTTTCGACTGTCTTCCTCTTGCTGCCCTCTT
  AAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCTCCTGAAATTACTTGTTTAGAGGACATTAGGAAA
  TTAGATAGGTTTTCTGAGCCTCCTGCTTTTGGGCCAGTGTGTGACCTGCTGTGGTCTGATCCCTTAGAGG
  ACTACGGCAGCGAGAAGACCCTGGAGCACTATACCCACAACACTGTCCGAGGCTGCTCCTACTTCTTCAG
  TTACCCTGCAGTTTGTGAATTTTTACAGAACAACAGTTTATTATCAATAATCAGAGCCCATGAAGCCCAG
  GATGCGGGGTACCGAATGTATAGGAAGAACCAAGCAACTGGCTTTCCGTCACTTATTACGATTTTCTCTG
  CCCCTAATTACCTAGATGTCTATAACAATAAAGCTGCAGTGTTGAAGTATGAAAACAATGTCATGAACAT
  CAGGCAGTTCAACTGTTCCCCACACCCCTACTGGCTCCCAAACTTCATGGATGTTTTCACGTGGTCTTTG
  CCTTTTGTTGGAGAGAAAGTGACAGAGATGCTGGTCAATATTCTCAACATATGCTCGGATGAAGAAATGA
  ACGTAACCGATGAAGAAGGAGCTACTACAGGTCGAAAAGAAGTCATCAAGAATAAAATCCGAGCCATTGG
  GAAAATGGCCCGGGTCTTTACGGTTCTTCGGGAAGAGAGTGAGAATGTGCTGACCCTCAAGGGCCTCACT
  CCCACAGGCACACTCCCACTGGGGGTCCTCTCTGGAGGAAAGCAGACCATTGAGACTGCCAAACAAGAAG
  CCGCAGAGGAGCGGGAAGCCATCAGAGGTTTTACAATTGCACACAGGATCCGAAGTTTTGAAGAAGCCCG
  AGGTCTAGACCGAATTAATGAGAGAATGCCACCCCGAAAAGAGGCTTCATATCATCATGATGCAGGGAGG
  ATGCACTCACACTCGCATCCGCCACACCCACAGGCGTCAAGAAGGACCGACCATGGAAAGAAAGCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224578 representing NM_008915
  Red=Cloning site Green=Tags(s)
  
  MSVRRPQFSTTERVIKAVPFPPTRRLTLKEVFENGKPKMDLLKNHLVKEGRVEEEVALKIINDGAAILKQ
  EKTMIEVEAPITVCGDVHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTL
  FLLRGNHECRHLTEYFTFKQECRIKYSEMVYDACMHTFDCLPLAALLNQQFLCVHGGMSPEITCLEDIRK
  LDRFSEPPAFGPVCDLLWSDPLEDYGSEKTLEHYTHNTVRGCSYFFSYPAVCEFLQNNSLLSIIRAHEAQ
  DAGYRMYRKNQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSL
  PFVGEKVTEMLVNILNICSDEEMNVTDEEGATTGRKEVIKNKIRAIGKMARVFTVLREESENVLTLKGLT
  PTGTLPLGVLSGGKQTIETAKQEAAEEREAIRGFTIAHRIRSFEEARGLDRINERMPPRKEASYHHDAGR
  MHSHSHPPHPQASRRTDHGKKAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008915
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008915.3, NP_032941.1
• RefSeq Size: 1948 bp
• RefSeq ORF: 1542 bp
• Locus ID: 19057
• UniProt ID: P48455
• Cytogenetics: 14 D2
• Gene Summary: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32.[UniProtKB/Swiss-Prot Function]