Abca3 (NM_013855) Mouse Tagged ORF Clone

CAT#: MG224548

Abca3 (tGFP-tagged) - Mouse ATP-binding cassette sub-family A (ABC1) member 3 (Abca3) transcript variant 1, (10ug)

Product Images

Circular map for MG224548

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abca3
• Synonyms: 1810036E22Rik; ABC-C; Abc3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224548 representing NM_013855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCTGCGGCAGCTCACCCTCCTACTCTGGAAGAACTATACCCTGAAGAAACGGAAGGTTCTAG
  TGACAGTCCTGGAGCTCTTCCTGCCCCTGCTGTTTTCTGGGATCCTGATTTGGCTTCGCTTGAAGATCCA
  GTCGGAGAATGTGCCCAATGCCACTGTTTACCCGGACCAGTCCATCCAGGAGCTGCCCCTGTTTTTCTCA
  TTCCCTCCACCTGGGGGGACCTGGGAGCTCGCCTATGTCCCTTCCCACAGCGATGCTGCCAGGACCATCA
  CGGAGACGGTGAAAAGGGAATTCATGATCAAGATGAGAGTGCACGGCTTCTCCTCTGAGAAGGACTTCGA
  GGACTACATTCGCTATGACAACCACTCCTCCAGCGTGCTGGCAGCTGTGGTATTTGAGCACAGCTTCAAC
  CACAGCCAGGACCCCCTGCCGCTGGCGGTGAAATACCACCTGCGCTTCAGTTACACACGAAGAAATTACA
  TGTGGACCCAAACAGGAAATATTTTCCTAAAAGAGACTGAAGGCTGGCATACCACTTCACTTTTTCCACT
  TTTTCCAAGCCCCGGACCGCGGGAACCTTCATCACCTGATGGCGGTGAACCTGGATACATTCGGGAAGGC
  TTCCTGGCCATGCAACATGCTGTAGACAAAGCCATCATGCGTTACCATGCCAACACCTCTGCACAGCAGC
  TGTTCCAGAAGCTCATGGTGATCACCAAAAGGTTCCCGTTCCCACCATACATCTCAGATCCATTCCTCAT
  TGCCATCCAGTACCAGCTTCCTCTGCTGCTCATGCTGAGTTTCACCTACACCTCCCTCACCATCATCCGG
  GCTGTGGTGCAGGAGAAGGAGAAAAAACTAAAGGAGTACATGCGCATGATGGGGCTTAACAGCTGGCTAC
  ACTGGAGCGCTTGGTTCCTCATGTTCTTCCTATTCTTCCTCATCGTGGTCTCCTTCATGACCCTCCTGTT
  CTGTGTCAAAGTGAAGAAGGACATAGCAGTCCTTTCCAACAGCGACCCCTCCCTGGTCCTGGCGTTCCTG
  CTGTGTTTTGCAATCTCCTCCATTTCCTTCAGCTTCATGGTCAGCACCTTCTTCAGTAAAGCCAACATAG
  CAGCAGCCGTGGGTGGCTTCCTGTACTTCTTCACCTATACCCCCTACTTTTTCGTGGCTCCTCGGTACAA
  CTGGATGACACTGAGTCAAAAGCTCTTGTCCTGTCTCCTATCTAATGTTGCCATGGCAATGGGAGCCCAG
  CTCATAGGAAAATTCGAAGCCAAAGGCACTGGCATCCAGTGGCGGGACCTCCTCAACCCTGTCAACGTGG
  ACGACAATTTCTGCTTTGGACAAGTGCTGGGGATGCTGCTGCTGGACTCAGCACTCTATGGCCTGGTGAC
  CTGGTACGTGGAGGCTGTCTTCCCAGGCCAGTTTGGCGTACCCCAGCCCTGGCACTTCTTCCTTATGCCC
  TCCTACTGGTGTGGGAACCCAAGAACTGTCGTAGGGAAGGAAGAGGAAGGCAGCGACCCAGAGAAAGCTC
  TCAGGAACGAGTACTTTGAAGCCGAGCCAGAGGACTTGGTAGCTGGAATCAAGATCAAGCATCTCTCCAA
  GGTGTTCCAAGTGGGCAATAAGGACAAGATGGGCATCAGAGACCTGACCCTGAACCTCTACGAAGGACAG
  ATAACAGTCCTCCTGGGCCACAATGGTGCTGGGAAGACCACGACCATGTCTCTGCTGACAGGCCTCTTCC
  CCCCTACCAGTGGACATGCTTATATTCATGGGTACGAAATTTCCCAAGACATGGCTCAGATCCGCAAGAG
  TTTGGGCCTGTGTCCCCAGCATGATGTACTGTTTGACAACCTGACAGTTGCCGAACATCTTTACTTCTAT
  GCACAGCTGAAAGGCCTCTCTCTCCAGAAGTGTCCTGAGGAAGTCAAGCAGATGCTACACATCCTCAGCC
  TGGAGGACAAGCGTGACTTACGGTCCAAGTTCCTGAGTGGGGGCATGAAGCGCAAGCTCTCCATTGGCAT
  CGCCCTCATAGCGGGCTCTAAGGTGCTGATGTTAGATGAGCCCACCTCAGGCATGGATGCAGTGTCCAGA
  AGGGCCATCTGGGACCTTCTCCAGCAGCAGAAGAGTGACCGCACCGTCCTGCTGACCACTCACTTCATGG
  ACGAAGCTGACCTGCTGGGAGACCGCATCGCCATCTTGGCCAAGGGGGAGCTCCAGTGCTGCGGGTCTTC
  ACTATTTCTCAAGCAGAAATACGGAGCTGGCTACCACATGACGCTGGTAAAAGAACCGCACTGCAATCCT
  GAAGGAATCTCTCAGCTGGTGCACCACCATGTCCCCAATGCCATGCTGGAGAGCCATGCTGGGGCTGAGC
  TGTCCTTTATTCTTCCCAAGGAGAGCACGCACAGGTTTGAAAGTCTGTTTGCTAAACTGGAAAAGAAGCA
  GAAGGAGTTGGGCATCGCAAGCTTCGGGGCCTCGGTCACCACCATGGAGGAAGTCTTCCTTCGGGTTGGT
  AAGCTGGTGGACACCAGCATGGACATTCAAGCCATTCAGCTTCCTGCTCTGCAGTACCAGCATGAGAGGC
  GGGCAAGCGACTGGGCTTTGGACAGTAACCTGTGTGGGGTGATGGACCCAACGAATGGCATTGGAGCCCT
  CATAGAGGAAGAGGAAGTTTTGGTCAAGCTCAACACAGGGCTTGCCCTCCACTGCCAGCAGTTCTGGGCC
  ATGTTCCTGAAGAAGGCTGCATACAGCTGGCGGGAATGGAAGATGGTGGCAGCTCAGGTCCTGGTCCCCC
  TGACCTGCCTCACCCTGGCCCTCCTGGCTATCCACTACACCTCAGAGATCTTTGATGACCCTCTCCTGAA
  GCTAAGCCTGAACGAGTATGGTAGAACGGTTGTGCCCTTCTCGGTCCCAGGCACATCCAGGCTGGCTCAG
  CAGCTGTCTGAGAATCTAAGGGACATGCTGCAGGCTGAGAGGCAGGAGCCCCGGGAAGTGCTAGGTGACC
  TAGAGGAGTTCCTGGTTTTCAGGGCCTCAGTGGAAGGAGGCGGCTTTAATGAGCGGTGCCTAGTAGCAAC
  ATCCTTCAAAGACAGAGGAGAGCTGACCGTGGTCACTGCCCTGTTCAACAACCAGGCATACCACTCCCCA
  GCCACTGCCCTGGCCATCGTGGACAACCTTCTGTTCAAGCTATTGTGTGGCCCTCAGGCCTCCATTGAGA
  TCTCCAACTACCCCCAGCCCCGGAACACCCTGCAGGTCGCCAAGGACCACTTCAATGAGGGCCGGAAGGG
  ATTTGACATTGCCCTCAATTTGCTCATCGCCATGGCATTTCTGGCCAGCACCTTTTCCATCCTGGCTGTC
  AGTGAGAGGGCCGTCCAGGCCAAGCACGTTCAGTTTGTGAGCGGTGTCCATGTGGCTACTTTCTGGTTCT
  CCGCTCTGTTGTGGGACCTCATCTCCTTCCTCGTTCCCAGTCTACTACTTCTGGTGGTGTTCCAAGCCTT
  CAATGTGCACGCCTTCACGAGGGATGGTCACATGGCTGACCTGCTGCTGCTGCTCATGCTCTACGGCTGG
  GCCATCATCCCCCTCATGTACCTCATGAGCTTCTTCTTCTCGGCTGCGTCCACAGCTTATACCAGGCTGA
  CCATATTCAACATCCTGTCGGGCATCGCTACCTTCATCATGGTCACTATCATGCGTATCCCAGCTGTGAA
  GTTAGAAGAACTTTCCAGAACCTTAGATCATGTGTTCTTGGTGCTGCCAAACCACTGCCTGGGGATGGCG
  GTCAGCAACTTCTATGAGAACTATGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCACTACT
  GCAAGAAGTACAACATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAGTTTGT
  GACTTCCATGGCTGCCTCAGGGGGCATCTACCTCACCCTGCTGTTCCTCATTGAGACCAACCTGCTGTGG
  CGACTGAGAACCTTCATCTGTGCCTTCCGGAGGAGGTGGACTCTGGCAGAACTGCAGAACCGGACATCAG
  TGCTGCCCGAGGACCAGGATGTAGCTGAGGAGAGGAGCCGAATCCTGGTCCCTAGCTTGGACTCCATGCT
  CGACACACCACTGATCATCAACGAGCTCTCCAAGGTGTATGACCAGCGAGCACCGCTCCTTGCCGTGGAC
  AGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAAAAACCA
  CAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGATGCCTTTGTTGGTGGTTACAGCAT
  CAGTTCTGACATCGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTGATCAC
  ATGACTGGCAGGGAGATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCAGAGCGGCTCATCAATGCCT
  GTGTGGAGAATACTCTGCGGGGTCTGCTGCTGGAACCGCACGCCAACAAACTAGTCAAGACTTACAGTGG
  TGGTAACAAACGCAAGCTAAGCACTGGCATTGCCCTCATTGGAGAGCCTGCAGTCATCTTCCTGGATGAG
  CCATCGACCGGCATGGACCCAGTGGCCCGGCGTCTACTCTGGGATACTGTAGCCCGGGCCCGAGAGTCTG
  GCAAAGCCATTGTCATCACCTCCCACAGTATGGAGGAGTGTGAAGCCTTATGTACCAGGCTGGCCATCAT
  GGTACAGGGCCAGTTCAAGTGCCTGGGCAGCCCACAGCACCTCAAGAGCAAGTTTGGCAGCGGCTACTCC
  CTGCAGGCCAAGGTCCGGAGCGAGGGGAAGCAAGACGCGCTAGAGGAGTTCAAGGCCTTTGTGGACCTGA
  CCTTCCCAGGCAGCATCCTAGAAGATGAGCACCAAGACATGGTCCATTATCATCTGCCTGGCTGTGACCT
  CAGCTGGGCCAAGGTATTTGGTATCCTGGAGAAAGCCAAGGAGAAGTATGGAGTGGATGATTACTCTGTG
  AGCCAGATCTCCCTGGAGCAGGTCTTTCTGAGCTTTGCCCACCTACAACCCCCCACCACTGAGGATGGCC
  GG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224548 representing NM_013855
  Red=Cloning site Green=Tags(s)
  
  MAVLRQLTLLLWKNYTLKKRKVLVTVLELFLPLLFSGILIWLRLKIQSENVPNATVYPDQSIQELPLFFS
  FPPPGGTWELAYVPSHSDAARTITETVKREFMIKMRVHGFSSEKDFEDYIRYDNHSSSVLAAVVFEHSFN
  HSQDPLPLAVKYHLRFSYTRRNYMWTQTGNIFLKETEGWHTTSLFPLFPSPGPREPSSPDGGEPGYIREG
  FLAMQHAVDKAIMRYHANTSAQQLFQKLMVITKRFPFPPYISDPFLIAIQYQLPLLLMLSFTYTSLTIIR
  AVVQEKEKKLKEYMRMMGLNSWLHWSAWFLMFFLFFLIVVSFMTLLFCVKVKKDIAVLSNSDPSLVLAFL
  LCFAISSISFSFMVSTFFSKANIAAAVGGFLYFFTYTPYFFVAPRYNWMTLSQKLLSCLLSNVAMAMGAQ
  LIGKFEAKGTGIQWRDLLNPVNVDDNFCFGQVLGMLLLDSALYGLVTWYVEAVFPGQFGVPQPWHFFLMP
  SYWCGNPRTVVGKEEEGSDPEKALRNEYFEAEPEDLVAGIKIKHLSKVFQVGNKDKMGIRDLTLNLYEGQ
  ITVLLGHNGAGKTTTMSLLTGLFPPTSGHAYIHGYEISQDMAQIRKSLGLCPQHDVLFDNLTVAEHLYFY
  AQLKGLSLQKCPEEVKQMLHILSLEDKRDLRSKFLSGGMKRKLSIGIALIAGSKVLMLDEPTSGMDAVSR
  RAIWDLLQQQKSDRTVLLTTHFMDEADLLGDRIAILAKGELQCCGSSLFLKQKYGAGYHMTLVKEPHCNP
  EGISQLVHHHVPNAMLESHAGAELSFILPKESTHRFESLFAKLEKKQKELGIASFGASVTTMEEVFLRVG
  KLVDTSMDIQAIQLPALQYQHERRASDWALDSNLCGVMDPTNGIGALIEEEEVLVKLNTGLALHCQQFWA
  MFLKKAAYSWREWKMVAAQVLVPLTCLTLALLAIHYTSEIFDDPLLKLSLNEYGRTVVPFSVPGTSRLAQ
  QLSENLRDMLQAERQEPREVLGDLEEFLVFRASVEGGGFNERCLVATSFKDRGELTVVTALFNNQAYHSP
  ATALAIVDNLLFKLLCGPQASIEISNYPQPRNTLQVAKDHFNEGRKGFDIALNLLIAMAFLASTFSILAV
  SERAVQAKHVQFVSGVHVATFWFSALLWDLISFLVPSLLLLVVFQAFNVHAFTRDGHMADLLLLLMLYGW
  AIIPLMYLMSFFFSAASTAYTRLTIFNILSGIATFIMVTIMRIPAVKLEELSRTLDHVFLVLPNHCLGMA
  VSNFYENYETRRYCTSSELAAHYCKKYNIQYQESFYAWSTPGVGKFVTSMAASGGIYLTLLFLIETNLLW
  RLRTFICAFRRRWTLAELQNRTSVLPEDQDVAEERSRILVPSLDSMLDTPLIINELSKVYDQRAPLLAVD
  RISLAVQKGECFGLLGFNGAGKTTTFKMLTGEETITSGDAFVGGYSISSDIGKVRQRMGYCPQFDALLDH
  MTGREMLVMYARLRGIPERLINACVENTLRGLLLEPHANKLVKTYSGGNKRKLSTGIALIGEPAVIFLDE
  PSTGMDPVARRLLWDTVARARESGKAIVITSHSMEECEALCTRLAIMVQGQFKCLGSPQHLKSKFGSGYS
  LQAKVRSEGKQDALEEFKAFVDLTFPGSILEDEHQDMVHYHLPGCDLSWAKVFGILEKAKEKYGVDDYSV
  SQISLEQVFLSFAHLQPPTTEDGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013855
• ORF Size: 5112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013855.3, NP_038883.2
• RefSeq Size: 6453 bp
• RefSeq ORF: 5115 bp
• Locus ID: 27410
• UniProt ID: Q8R420
• Cytogenetics: 17 A3.3
• Gene Summary: Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.[UniProtKB/Swiss-Prot Function]