Akap7 (NM_018747) Mouse Tagged ORF Clone

CAT#: MG224507

Akap7 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 7 (Akap7), (10ug)

Product Images

Circular map for MG224507

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Akap7
• Synonyms: 6430401D08; AI662165; AKAP15; Akap18; BB170514
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224507 representing NM_018747
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTTGCTGCTGTAGATATTCAAGATGACTGTGGAAGCCCTGATGTACCTCAAGCAAACCCAAAGA
  GAAGCAAAGAAGAAGAAGAAGATAGAGGCGACAAGAATGATCATGTGAAGAAGAGGAAAAAAGCCAAAAA
  AGATTATCAGCCCAACTATTTCCTGTCCATTCCGATCACCAACAAAAAGATTACAACCGGAATTAAAGTC
  TTGCAAAATTCGATACTACAGCAGGATAAGCGACTGACCAAAGCCATGGTGGGCGACGGCTCCTTTCATA
  TTACCTTGCTAGTGATGCAGTTATTAAACGAAGATGAAGTAAATATCGGTACCGATGCACTTTTGGAATT
  GAAGCCATTCGTTGAGGAGATCCTTGAAGGGAAACACTTGGCTTTGCCCTTCCAAGGGATTGGCACTTTC
  CAAGGTCAGGTTGGCTTTGTGAAGCTGGCAGATGGAGACCATGTTAGCGCACTCCTGGAGATAGCAGAGA
  CTGCGAAAAGAACATTTCGGGAAAAAGGCATCCTAGCTGGAGAAAGCAGAACTTTTAAGCCCCACCTGAC
  CTTTATGAAGCTGTCCAAAGCACCGATGCTCAGGAAGAAGGGAGTGAGAAAAATCGAGCCTGGATTGTAT
  GAACAATTTATCGACCACAGATTTGGAGAAGAACTATTGTACCAGATAGATCTCTGCTCCATGCTGAAGA
  AAAAACAGAGCAATGGTTATTACCACTGTGAGTCTTCCATCGTGATCGGTGAGAAGGACCGAAGGGAGCC
  TGAGGATGCTGAACTGGTCAGGCTCAGTAAGAGGCTGGTGGAGAACGCCGTGCTCAAGGCTGTCCAGCAG
  TACCTAGAAGAGACGCAGAACAAAAAGCAGCCAGGGGAGGGGAACTCCACCAAAGCTGAGGAGGGAGATC
  GGAATGGTGATGGCAGTGATAACAACCGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224507 representing NM_018747
  Red=Cloning site Green=Tags(s)
  
  MPFAAVDIQDDCGSPDVPQANPKRSKEEEEDRGDKNDHVKKRKKAKKDYQPNYFLSIPITNKKITTGIKV
  LQNSILQQDKRLTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLALPFQGIGTF
  QGQVGFVKLADGDHVSALLEIAETAKRTFREKGILAGESRTFKPHLTFMKLSKAPMLRKKGVRKIEPGLY
  EQFIDHRFGEELLYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRREPEDAELVRLSKRLVENAVLKAVQQ
  YLEETQNKKQPGEGNSTKAEEGDRNGDGSDNNRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018747
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018747.5
• RefSeq Size: 3140 bp
• RefSeq ORF: 945 bp
• Locus ID: 432442
• UniProt ID: Q7TN79
• Cytogenetics: 10 A4
• Gene Summary: Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity).[UniProtKB/Swiss-Prot Function]