Xylb (NM_001033209) Mouse Tagged ORF Clone

CAT#: MG224257

Xylb (tGFP-tagged) - Mouse xylulokinase homolog (H. influenzae) (Xylb), (10ug)

Product Images

Circular map for MG224257

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xylb
• Synonyms: AI132223; AI327421
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224257 representing NM_001033209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCACGGACGCACAGACGGGCGGCGGGAACCCCCAGGGCCCTGGCGGAGCGCGCGGGGCGCCGCT
  GTTGCCTGGGCTGGGACTTCAGCACGCAGCAGGTTAAAGTTGTTGCTGTTGATGCAGAGTTGAACGTCTT
  CTATGAGGACAGCGTGCATTTTGACAGAGACCTTCCGGAATTTGGAACTCAGGGCGGTGTCCATGTACAC
  AAGGACAGGCTGACGGTCACCTCTCCGGTCCTGATGTGGGTCCAGGCTCTGGACCTGATCCTGGGGAAGA
  TGAAGTCTTCTGGCTTTGACTTCTCTCAAGTTCTAGCCTTATCTGGAGCAGGCCAGCAACACGGAAGTGT
  TTACTGGAAGACTGGAGCCAGCCTGGCCCTGTCAAGCTTGTCACCAGCTCTTCCATTACATCAGCAGCTG
  CAGAGCTGCTTCTCCATCAGCGACTGCCCAATATGGATGGACTCCAGTACCACAGCCCAGTGCCACCAGC
  TGGAGGCTGCCGTGGGTGGGGCCCAGGCTCTCAGCTGCCTCACAGGATCCCGGGCCTATGAGCGTTTCAC
  GGGGAACCAGATAGCGAAGCTTTTCCAGAAGAACCCTGAGGCCTACTCACACTCTGAGAGAATTTCCTTG
  GTCAGCAGCTTTGCTGCCTCGCTGTTCCTGGGTGGGTACTCGCCCATCGACTACAGTGATGGGTCCGGAA
  TGAATCTGTTGCAGATCCAGGAAAAAGTCTGGTCCCAGGCTTGTCTGGATGTCTGTGCTCCCCATTTGGA
  GGAGAAGCTGGGCTCACCGGTCCCTTCCTGTTCAGTTGTGGGAACCATTTCTTCCTACTACGTCCAGCGC
  TATGGATTCCCTCCAGGATGCAAAGTGGTGGCCTTCAGTGGGGACAACCCAGCGTCACTGGCCGGCATGA
  GGCTGGAGGAAGGAGATATTGCGGTCAGCCTGGGCACCAGCGACACCCTGTTTCTCTGGCTCCAGAAGCC
  CATGCCTGCCTTGGAAGGCCACATCTTCTGCAACCCAGTGGATCCGCAGCATTACATGGCGCTTCTATGC
  TTTAAAAACGGCTCCCTCATGAGAGAGAAGATCCGAGATGAGTCGGCCTCCTGTTCCTGGAACAAGTTCT
  CTAAGGCCCTGAAGTCCACGGCGATGGGGAACAACGGAAACTTGGGTTTTTATTTCGATGTAATGGAGAT
  CACCCCTGAAATTATTGGGCGTCATAGGTTTAATGCGGAAAACATGGAGGTTTCAGCATTCCCTGGGGAT
  GTGGAGATCCGAGCACTGATTGAAGGACAATTCATGGCCAAGAGGATTCATGCAGAAGGCCTAGGCTACA
  GAGTCATGCCCAAGACAAAGATTCTGGCCACGGGAGGAGCATCTCACAATAAAGACATCTTGCAGGTGCT
  TGCAGATGTTTTTGGGGCCCCCGTATACGTCATAGACACCACCAGTTCAGCCTGCGTCGGCTCCGCATAC
  CGAGCTTTTCATGGCCTTGCAGGAGGGACAGGTGTGGCCTTTTCGGAGGTTGTGAAATCAGCACCTCAGC
  CCAGCCTGGCTGCCACCCCCAACCCAGGAGCTTCCCAGGTCTATGCGGCCCTTCTCCCTCGATACTCTGC
  ACTGGAACAGAGAATCTTGTCCACCGCCCAGAGGCCTCTGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224257 representing NM_001033209
  Red=Cloning site Green=Tags(s)
  
  MDARTHRRAAGTPRALAERAGRRCCLGWDFSTQQVKVVAVDAELNVFYEDSVHFDRDLPEFGTQGGVHVH
  KDRLTVTSPVLMWVQALDLILGKMKSSGFDFSQVLALSGAGQQHGSVYWKTGASLALSSLSPALPLHQQL
  QSCFSISDCPIWMDSSTTAQCHQLEAAVGGAQALSCLTGSRAYERFTGNQIAKLFQKNPEAYSHSERISL
  VSSFAASLFLGGYSPIDYSDGSGMNLLQIQEKVWSQACLDVCAPHLEEKLGSPVPSCSVVGTISSYYVQR
  YGFPPGCKVVAFSGDNPASLAGMRLEEGDIAVSLGTSDTLFLWLQKPMPALEGHIFCNPVDPQHYMALLC
  FKNGSLMREKIRDESASCSWNKFSKALKSTAMGNNGNLGFYFDVMEITPEIIGRHRFNAENMEVSAFPGD
  VEIRALIEGQFMAKRIHAEGLGYRVMPKTKILATGGASHNKDILQVLADVFGAPVYVIDTTSSACVGSAY
  RAFHGLAGGTGVAFSEVVKSAPQPSLAATPNPGASQVYAALLPRYSALEQRILSTAQRPLE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033209
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033209.3, NP_001028381.1
• RefSeq Size: 3860 bp
• RefSeq ORF: 1656 bp
• Locus ID: 102448
• UniProt ID: Q3TNA1
• Cytogenetics: 9 F3
• Gene Summary: Phosphorylates D-xylulose to produce D-xylulose 5-phosphate, a molecule that may play an important role in the regulation of glucose metabolism and lipogenesis.[UniProtKB/Swiss-Prot Function]