Map3k8 (NM_007746) Mouse Tagged ORF Clone

CAT#: MG224251

Map3k8 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 8 (Map3k8), (10ug)

Product Images

Circular map for MG224251

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Map3k8
• Synonyms: c-COT; Cot; Cot/Tpl2; Est; Estf; Tpl-2; Tpl2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224251 representing NM_007746
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTACATGAGCACTGGAAGTGACGAGAAAGAAGAAATTGATTTATTAATTAAGCATTTAAACGTGT
  CAGAAGTCATAGACATAATGGAGAACCTTTATGCAAGTGAAGAGCCAGGAGTGTATGAGCCCAGTCTGAT
  GACCATGTATCCAGACAGCAATCAAAATGAGGAACGTTCGGAGTCACTGCTTCGGAGTGGCCAGGAGGTT
  CCCTGGCTGTCATCTGTCAGATATGGGACCGTGGAGGATCTGCTTGCATTTGCAAACCATGTCTCCAATA
  TGACAAAGCATTTTTATGGACGTCGACCACAAGAATGTGGAATTTTATTAAATATGGTAATCAGTCCCCA
  GAATGGCCGCTACCAAATCGATTCGGATGTTCTCCTTGTTCCTTGGAAGCTGACATACAGGAACATCGGC
  TCTGGTTTCGTTCCTCGGGGGGCCTTTGGAAAAGTATACTTAGCCCAAGACATGAAGACAAAGAAAAGAA
  TGGCGTGCAAACTGATCCCTATAGATCAGTTTAAGCCATCGGATGTGGAAATCCAGGCCTGTTTCCGGCA
  TGAGAACATTGCTGAGTTATATGGTGCCGTCCTATGGGGCGATACTGTCCATCTCTTTATGGAAGCCGGC
  GAGGGAGGGTCTGTTCTGGAGAAACTGGAGAGTTGTGGGCCCATGAGAGAATTTGAAATTATTTGGGTGA
  CGAAGCACATTCTCAAGGGACTTGATTTTCTGCACTCCAAGAAAGTGATCCACCATGATATTAAACCTAG
  CAACATTGTATTCATGTCTACAAAAGCTGTTTTGGTAGATTTTGGCCTGAGTGTTAAGATGACTGAAGAT
  GTCTATCTTCCCAAGGACCTCCGGGGAACAGAGATATACATGAGCCCAGAGGTGATCCTATGTCGGGGCC
  ATTCCACAAAAGCCGACATCTACAGCCTTGGAGCCACACTCATCCACATGCAGACAGGCACCCCACCCTG
  GGTGAAGCGCTACCCTCGATCAGCCTATCCCTCCTACCTATACATTATCCACAAGCAGGCACCTCCCCTG
  GAAGACATCGCTGGTGACTGCAGTCCAGGCATGAGGGAGCTGATAGAAGCTGCCCTGGAGAGGAACCCCA
  ACCACCGCCCCAAAGCAGCAGACCTACTGAAACATGAAGCCCTGAATCCCCCAAGAGAGGACCAGCCACG
  ATGTCAGAGTCTGGACTCTGCCCTCTTTGAACGGAAGAGGCTGCTGAGCAGGAAGGAACTACAACTTCCT
  GAGAACATTGCTGATTCATCATGCACAGGCAGCACCGAAGAGTCTGAAGTGCTCAGGAGACAGCGTTCCC
  TCTACATTGACCTCGGAGCTCTGGCTGGCTACTTCAATATTGTTCGTGGGCCACCAACCCTGGAATACGG
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224251 representing NM_007746
  Red=Cloning site Green=Tags(s)
  
  MEYMSTGSDEKEEIDLLIKHLNVSEVIDIMENLYASEEPGVYEPSLMTMYPDSNQNEERSESLLRSGQEV
  PWLSSVRYGTVEDLLAFANHVSNMTKHFYGRRPQECGILLNMVISPQNGRYQIDSDVLLVPWKLTYRNIG
  SGFVPRGAFGKVYLAQDMKTKKRMACKLIPIDQFKPSDVEIQACFRHENIAELYGAVLWGDTVHLFMEAG
  EGGSVLEKLESCGPMREFEIIWVTKHILKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVKMTED
  VYLPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPL
  EDIAGDCSPGMRELIEAALERNPNHRPKAADLLKHEALNPPREDQPRCQSLDSALFERKRLLSRKELQLP
  ENIADSSCTGSTEESEVLRRQRSLYIDLGALAGYFNIVRGPPTLEYG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007746
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007746.2, NP_031772.1
• RefSeq Size: 2507 bp
• RefSeq ORF: 1404 bp
• Locus ID: 26410
• UniProt ID: Q07174
• Cytogenetics: 18 2.73 cM
• Gene Summary: Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.[UniProtKB/Swiss-Prot Function]