Gfod1 (NM_001033399) Mouse Tagged ORF Clone

CAT#: MG224238

Gfod1 (tGFP-tagged) - Mouse glucose-fructose oxidoreductase domain containing 1 (Gfod1), (10ug)

Product Images

Circular map for MG224238

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gfod1
• Synonyms: 9630032O13; AI850995
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224238 representing NM_001033399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCCGGGGTGGGCGTGTTCGGCACCAGCCTCACAGCCCGAGTCATCATCCCGCTGCTGAAAGATG
  AGGGCTTCGCGGTGAAGGCGCTGTGGGGCCGCACGCAGGAAGAAGCCGAGGAGCTGGCCAAGGAGATGAG
  CGTTCCTTTCTACACCAGCCGCATCGACGAGGTGCTCCTGCACCAGGATGTGGACTTGGTGTGCATCAAC
  CTGCCGCCGCCGCTCACTAGGCAGATCGCTGTCAAAACCCTGGGAATAGGCAAGAATGTCATCTGTGACC
  GTACCGCCACGCCGCTGGACGCCTTCCGCATGATGTCAGCGGCCCACTACTACCCGAAGCTCATGAGCAT
  CATGGGCAACGTGCTGCGCTTCCTCCCAGCCTTCGTACGCATGAAGCAGCTGATTGAGGAGGGCTACGTG
  GGCGAGCTGCTGGTGTGCGAGGTGCAGGTGCACAGCGGTAGTCTGCTGGGCAAGAAATACAACTGGAGCT
  GCGACGACCTGATGGGTGGTGGCGGCCTGCACTCCGTGGGCACCTACATCATTGACCTGCTCACCTTCCT
  CACGGGCCAGAAGGCTGTCAAGGTCCACGGGCTGCTCAAGACCTTTGTCAAGCAGACGGACCACATCAAG
  GGCATCCGTCAGATCACCAGTGATGACTTCTGCACCTTCCAGATGGTGCTGGAGGGTGGAGTGTGCTGCA
  CCGTTACCCTGAACTTCAATGTGCCTGGAGAGTTCAAGCAGGACGTGACCGTGGTGGGTTCCGCTGGGCG
  GCTCCTGGCGGTGGGCACCGACCTCTATGGACAGCGCAATAGCGCCCCAGAGCAGGAGCTGCTGCTTCAG
  GACACTACATCCGTGAGCAACTCCCTGCTGCCTGAGAAGGCATTCAGCGACATCCCCTCGCCCTACCTGC
  GGGGCACCATGAAGATGATGCAGGCAGTGCGCCAGGCCTTCCAGGATCAAGATGACCGGCGCACGTGGGA
  CGGCCGGCCTCTTACCATGGCTGCCACCTTCGACGACTGCCTGTACGCTCTTTGCGTGGTAGACACCATC
  AAGAGGTCCAGCCAGACTGGAGAGTGGCAGAACATCGCCATCATGACCGAGGAGCCAGAGCTGAGCCCTG
  CCTACCTGATCAGTGAGGCCATGCGCAGAAGCAGGATGTCCCTTTACTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224238 representing NM_001033399
  Red=Cloning site Green=Tags(s)
  
  MLPGVGVFGTSLTARVIIPLLKDEGFAVKALWGRTQEEAEELAKEMSVPFYTSRIDEVLLHQDVDLVCIN
  LPPPLTRQIAVKTLGIGKNVICDRTATPLDAFRMMSAAHYYPKLMSIMGNVLRFLPAFVRMKQLIEEGYV
  GELLVCEVQVHSGSLLGKKYNWSCDDLMGGGGLHSVGTYIIDLLTFLTGQKAVKVHGLLKTFVKQTDHIK
  GIRQITSDDFCTFQMVLEGGVCCTVTLNFNVPGEFKQDVTVVGSAGRLLAVGTDLYGQRNSAPEQELLLQ
  DTTSVSNSLLPEKAFSDIPSPYLRGTMKMMQAVRQAFQDQDDRRTWDGRPLTMAATFDDCLYALCVVDTI
  KRSSQTGEWQNIAIMTEEPELSPAYLISEAMRRSRMSLYC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033399
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033399.4, NP_001028571.1
• RefSeq Size: 6654 bp
• RefSeq ORF: 1173 bp
• Locus ID: 328232
• UniProt ID: Q3UHD2
• Cytogenetics: 13 A4