Brdt (NM_001079873) Mouse Tagged ORF Clone

CAT#: MG224116

Brdt (tGFP-tagged) - Mouse bromodomain testis-specific (Brdt) transcript variant 2, (10ug)

Product Images

Circular map for MG224116

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Brdt
• Synonyms: 7420412D09Rik; Brd; Brd6; Fsr; Fsrg3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224116 representing NM_001079873
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGCCAAGTCGACAAACAGCTATTGTTAACCCCCCTCCACCAGAGTACATAAACACTAAGAAGA
  GTGGGCGGTTGACGAATCAGCTGCAGTTCCTACAGAGGGTTGTGCTGAAGGCCCTGTGGAAGCACGGCTT
  CTCTTGGCCTTTCCAACAGCCGGTGGACGCCGTGAAACTAAAGCTGCCTGACTATTACACCATCATAAAA
  ACCCCAATGGATTTAAATACAATTAAGAAGCGGTTGGAAAATAAGTATTATGAGAAGGCTTCCGAATGCA
  TAGAAGACTTCAACACAATGTTCTCAAACTGCTACTTATACAACAAGACTGGAGATGACATTGTTGTTAT
  GGCCCAAGCTCTAGAGAAGTTATTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTGGGT
  GGTAAGGAGAGAATAAAGAAAGATATCCAACAAAAGATAGCGGTTTCCTCTGCTAAGGAACAAATCCCCT
  CCAAAGCGGCAGAGAATGTCTTTAAGCGGCAAGAGATTCCTTCTGGGCTTCCTGACATCTCTCTCTCGCC
  CTTAAACATGGCACAAGAAGCTCCCCCGATCTGTGACTCGCAGTCTCTGGTCCAAATTACAAAGGGTGTG
  AAGAGGCGAGCAGACACAACAACTCCTACCACTTCCATAGCTAAAGCAAGTAGCGAATCTCCTCCGACAC
  TTAGAGAAACAAAGCCAGTGAACATGCCAGTAAAAGAAAATACAGTAAAGAACGTTTTGCCAGACTCTCA
  GCAACAACACAAAGTTTTAAAAACAGTCAAAGTAACGGAGCAATTAAAGCACTGCAGCGAGATTCTTAAA
  GAAATGCTTGCCAAGAAACATTTGCCGTATGCATGGCCCTTCTATAATCCTGTGGATGCTGATGCTTTGG
  GACTCCACAACTACTATGACGTTGTCAAAAATCCCATGGATCTCGGAACGATCAAGGTAAATACTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224116 representing NM_001079873
  Red=Cloning site Green=Tags(s)
  
  MSLPSRQTAIVNPPPPEYINTKKSGRLTNQLQFLQRVVLKALWKHGFSWPFQQPVDAVKLKLPDYYTIIK
  TPMDLNTIKKRLENKYYEKASECIEDFNTMFSNCYLYNKTGDDIVVMAQALEKLFMQKLSQMPQEEQVVG
  GKERIKKDIQQKIAVSSAKEQIPSKAAENVFKRQEIPSGLPDISLSPLNMAQEAPPICDSQSLVQITKGV
  KRRADTTTPTTSIAKASSESPPTLRETKPVNMPVKENTVKNVLPDSQQQHKVLKTVKVTEQLKHCSEILK
  EMLAKKHLPYAWPFYNPVDADALGLHNYYDVVKNPMDLGTIKVNTA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079873
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079873.1, NP_001073342.1
• RefSeq Size: 1588 bp
• RefSeq ORF: 981 bp
• Locus ID: 114642
• UniProt ID: Q91Y44
• Cytogenetics: 5 E5
• Gene Summary: The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]