Ablim2 (NM_001177696) Mouse Tagged ORF Clone

CAT#: MG224095

Ablim2 (tGFP-tagged) - Mouse actin-binding LIM protein 2 (Ablim2) transcript variant 1, (10ug)

Product Images

Circular map for MG224095

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ablim2
• Synonyms: AI606905; C230091L11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224095 representing NM_001177696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCAGTGTCGCAGCCCCAGGCTGCTCATGCCCCACTGGAGAAGCCGGCCAGCACTGCGATCCTGT
  GTAATACCTGTGGGAATGTGTGCAAGGGAGAGGTGCTGCGTGTGCAGAACAAGTACTTCCACATCAGGTG
  CTTTGTGTGCAAAGCGTGTGGCTGTGACCTGGCTGAGGGTGGCTTCTTCGTGAGGCAGGGTGAGCACATT
  TGCACACGTGACTACCAGCGGCTCTATGGCACTCGCTGCTTCAGCTGTGACCGCTTCATTGAAGGCGAAG
  TGGTGTCGGCGCTTGGGAAGACCTACCACCCTGACTGCTTCGTGTGTGCTGTCTGCAGGCTGCCCTTTCC
  TCCTGGGGACCGTGTGACCTTCAACGGGAAGGAGTGTATGTGCCAGAAGTGTTCCCCACCCACATTGCTG
  GGCAACAGTGCTCACGTGGCCCAGGGCCTCCGGAGTTGTGGGGGCTGTGGCTTAGAAATCAAGAATGGCC
  AGGCCCTGGTGGCTTTGGACAAGCACTGGCACCTGGGATGCTTCAAGTGCAAGACTTGTGGGAAACTCCT
  CAATGCAGAGTACATCAGCAAGGACGGGCTGCCCTACTGCGAGGCTGATTATCACAGCAAGTTTGGCATC
  CGCTGCGACGGCTGTGAGAAGTACATCACGGGCCGGGTGCTAGAGGCCGGAGAGAAGCACTACCACCCTT
  CATGTGCGCTATGCGTCAGGTGCGGCCAGATGTTCTCAGAGGGCGAGGAGATGTACCTGCAAGGCTCCTC
  CATCTGGCATCCGGCATGTCGACAGGCAGCCAGGACTGAAGACAAGAGCAAGATGCACAGCTCTGTTTGC
  AGCCAGCCCTGCCCGGGCACCCTGCCCTGTGCCTTACAGGAAACCAGGACTTCTTCGGAGAGCATTGTCT
  CCGTGCCTGCCTCCAGCACGTCAGGGTCCCCGAGCCGTGTGATCTACGCAAAGCTTGGCGATGAGATCCT
  GGACTACAGGGACTTGGCTGCTCTTCCCAAAAACAAAGCGATCTACAACATCGACCGCCCCGACATGATC
  TCCTATTCGCCCTACATTAGCCACTCGGCCGTGGGGGACAGGCAGAGCTATGGCGAGGGGGATCAGGATG
  ATCGGTCCTACAAGCAGTGCAGGACCTCCAGCCCCAGCTCTGCCGGCTCAGTCAGCCTCGGACACTACAC
  CCCAACCTCACGGTCACCCCAGCACTACAGTCGTCCAGCTGGTACTGTGAGTGTTGGTACCAGTAGCTGT
  CTGTCCCTGTCCCAACACCCAAGCCCTACATCCGTGTTCAGACATCATTACATCCCCTACTTCCGAGGCA
  GCGAAAGTGGCCGTAGCACCCCGAGTCTCTCAGTGCACTCCGACAGCAGACCTCCTTCCTCTACCTACCA
  GCAGGCTCCGAGACACTTCCACGTCCCAGACACTGGCGTAAAAGATAACATCTATAGGAAACCCCCTATC
  TACAAACAGCACGCTGCCAGGCGATTGGACGTGGAGGACAGCAGCTTTGACCAGGACAGCAGGAAGAAGA
  CAACCTGGCTGCTTCTCAAGGGGGATGCAGACACCAGAACTAACTCTCCAGACCTGGACAGTCAGTCGCT
  GTCCCTCAGCAGCGGGACTGACCAGGAACCTCTCCAAAGAATGGCAGGGGACAGCCTCTACTCACGCTTT
  CCTTATTCCAAACCTGACACTCTTCCAGGACCCAGGAAGGATGGCCTGGACCTCCGGAATGCCAACCTGG
  CCCCTTGTGGAGCAGACCCGGATGCCAGCTGGGGCACGCGAGAGTATAAGATATATCCCTATGATTCCCT
  CATCGTAACAAACCGAATTCGTGTGAAACTGCCCAAAGACGTGGACCGGACACGGCTGGAGAGACACCTA
  TCACCTGAGGAGTTCCAAGAAGTGTTTGGGATGAGCATCGAGGAGTTTGACCGCCTGGCCCTGTGGAAGA
  GGAATGACCTCAAGAAGAAAGCCTTGCTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224095 representing NM_001177696
  Red=Cloning site Green=Tags(s)
  
  MSAVSQPQAAHAPLEKPASTAILCNTCGNVCKGEVLRVQNKYFHIRCFVCKACGCDLAEGGFFVRQGEHI
  CTRDYQRLYGTRCFSCDRFIEGEVVSALGKTYHPDCFVCAVCRLPFPPGDRVTFNGKECMCQKCSPPTLL
  GNSAHVAQGLRSCGGCGLEIKNGQALVALDKHWHLGCFKCKTCGKLLNAEYISKDGLPYCEADYHSKFGI
  RCDGCEKYITGRVLEAGEKHYHPSCALCVRCGQMFSEGEEMYLQGSSIWHPACRQAARTEDKSKMHSSVC
  SQPCPGTLPCALQETRTSSESIVSVPASSTSGSPSRVIYAKLGDEILDYRDLAALPKNKAIYNIDRPDMI
  SYSPYISHSAVGDRQSYGEGDQDDRSYKQCRTSSPSSAGSVSLGHYTPTSRSPQHYSRPAGTVSVGTSSC
  LSLSQHPSPTSVFRHHYIPYFRGSESGRSTPSLSVHSDSRPPSSTYQQAPRHFHVPDTGVKDNIYRKPPI
  YKQHAARRLDVEDSSFDQDSRKKTTWLLLKGDADTRTNSPDLDSQSLSLSSGTDQEPLQRMAGDSLYSRF
  PYSKPDTLPGPRKDGLDLRNANLAPCGADPDASWGTREYKIYPYDSLIVTNRIRVKLPKDVDRTRLERHL
  SPEEFQEVFGMSIEEFDRLALWKRNDLKKKALLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177696
• ORF Size: 1992 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177696.1, NP_001171167.1
• RefSeq Size: 3726 bp
• RefSeq ORF: 1995 bp
• Locus ID: 231148
• Cytogenetics: 5 B3
• Gene Summary: May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.[UniProtKB/Swiss-Prot Function]