Zfp277 (NM_172575) Mouse Tagged ORF Clone

CAT#: MG224030

Zfp277 (tGFP-tagged) - Mouse zinc finger protein 277 (Zfp277) transcript variant 1, (10ug)

Product Images

Circular map for MG224030

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zfp277
• Synonyms: 2410017E24Rik; NIRF4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224030 representing NM_172575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTAGTGAGGTCCAGGGGCTTCCTGTGGACCCCCGGGAGGCTGGGGTCCGCGGGGTCCGCGGCG
  GAATCGGACACACAGATCATGAAGGACATCTGGGTTCTTTCCAGCTTCTGGCTACTATAAATAAGGCTGC
  TATGAACATAGTGGAGCATGTGTCCTTCTTACCAGTTGGAACATCTTCTGGATATATTGCAGCATCCTCC
  GGTATTACTATGTCCAATTTTCTGAGGAACCGCCAGACTGATTTCCAGAGTGGTTGTACAAGCTTGCAAT
  CCCACCAACAATGGAGGAGTGTTCCTCTTTCTCCACATCCTCACCAGCATCTGCTGTCACCTGAGTTTTT
  GATCTTAGCCATTCTGACGGGTGTGAGGTGGAATCTCAGGATTGCTTTGATTTGCATTTCCCTGATGATT
  AAGGATGCTGAACATTTTTTCAGGTGCTTCTCAGCCATTTGGTATTCCTCAGACAGTAAAGACTGTATCC
  TGGAGCCGCTTTCCCTGCCAGAGAGTCCAGGTGGCACCACCGCTTTAGAAGGGTCTCCATCTGTGCCTTG
  TATTTTCTGTGAAGAACATTTTCCCATGGCTGAACAAGACAAACTTTTGAAGCACATGATTATCGAGCAT
  AAGATTGTCATAGCTGATGTGAAGCTCGTTGCAGATTTCCGAAGGTACATTTTATACTGGAGGAAAAGGT
  TCACTGAACAGCCCATCACTGATTTTTGCAGCGTGATAAGAATCAATTCCACAGCTCCATTTGAGGAACA
  AGACAACTATTATTTGTTATGTGATGCATTACCAGAAGATAGAATCCTCAGAGAAGAGCTTCAAAAACAC
  AAACTGAAAGAAGTTCTGGATCAGCAACAGCGAGAGCGAAATGATACAAGCTTTCATGGTGTGTGCATGT
  TTTGCAGTGAGGAATTTCGCGGAAACAGATCTGTACTGTTGAACCACATGGCCAGAGAACATGCTTTCAA
  CATTGGTTTGCCAGACAACATTGTAAATTGCGCTGAATTCCTATGCACATTGCAAAAGAAACTGGACAAT
  TTACAGTGTTTGTACTGTGAGAAGACCTTCAGAGACAAAAACACCCTTAAAGATCACATGAGAAAAAAGC
  AGCATCGTAGGATCAATCCAAAAAACAGAGAATATGACCGATTCTATGTCATCAATTACTTGGAACTCGG
  GAAGTCATGGGAAGAGGTGCAGTCAGAAGACGACCGCGAGCTGCTGGACCTTCAGGAAGACGACTGGTCT
  GATTGGCAGGAGTACCCAGTCTCTGCTGTGTGTCTCTTTTGTGAAAAGCAAGAGGAGACAATAGATAAAC
  TCTATGTGCACATGAAGGACACACATGAGTTTGATCTCCTCAGAATAAAGTCAGAGCTTGGATTAAATTT
  CTATCAGCAAGTGAAGCTGGTCAATTTCATTAGAAGGCAAGTTCACCAATGCAAGTGCTACAGTTGCCAT
  GTAAAGTTCAAATCCAAAGCAGATTTAAGGACTCACATGGAAGACACTAAACACACCTCACTGCTTCCTG
  ACAGGAAGACATGGGATCAACTGGAGTATTATTTCCCCACCTATGAGAATGACACTCTCCTGTGTACACT
  GTCTGACAGTGAAAGTGACCTAACAGCTCAAGAGCAAACGGAAAATGTCCCCGTCATCAGTGAAGATACG
  TCCAGACTGTGTGCTTTAAAACAGAGCAGTGTTCTCAACCAATTGCTACTCCAGGGGTGCCTGGAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224030 representing NM_172575
  Red=Cloning site Green=Tags(s)
  
  MAASEVQGLPVDPREAGVRGVRGGIGHTDHEGHLGSFQLLATINKAAMNIVEHVSFLPVGTSSGYIAASS
  GITMSNFLRNRQTDFQSGCTSLQSHQQWRSVPLSPHPHQHLLSPEFLILAILTGVRWNLRIALICISLMI
  KDAEHFFRCFSAIWYSSDSKDCILEPLSLPESPGGTTALEGSPSVPCIFCEEHFPMAEQDKLLKHMIIEH
  KIVIADVKLVADFRRYILYWRKRFTEQPITDFCSVIRINSTAPFEEQDNYYLLCDALPEDRILREELQKH
  KLKEVLDQQQRERNDTSFHGVCMFCSEEFRGNRSVLLNHMAREHAFNIGLPDNIVNCAEFLCTLQKKLDN
  LQCLYCEKTFRDKNTLKDHMRKKQHRRINPKNREYDRFYVINYLELGKSWEEVQSEDDRELLDLQEDDWS
  DWQEYPVSAVCLFCEKQEETIDKLYVHMKDTHEFDLLRIKSELGLNFYQQVKLVNFIRRQVHQCKCYSCH
  VKFKSKADLRTHMEDTKHTSLLPDRKTWDQLEYYFPTYENDTLLCTLSDSESDLTAQEQTENVPVISEDT
  SRLCALKQSSVLNQLLLQGCLEN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172575
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172575.3, NP_766163.2
• RefSeq Size: 2454 bp
• RefSeq ORF: 1752 bp
• Locus ID: 246196
• Cytogenetics: 12 B1