Trim43c (NM_001177858) Mouse Tagged ORF Clone

CAT#: MG224020

Trim43c (tGFP-tagged) - Mouse tripartite motif-containing 43C (Trim43c), (10ug)

Product Images

Circular map for MG224020

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trim43c
• Synonyms: EG666731; Trim43
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224020 representing NM_001177858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCAGACAATTTACAAGACCCTCAGGAGGAAACACTCACCTGCTCCATCTGCCAGGGTATCTTTA
  TGGATCCAGTTTATTTAAGGTGTGGCCATAAGTTCTGCGAGACATGTCTCTTACTTTTTCAAGAAGACAT
  CAAATTTCCTGCCTACTGCCCCACGTGTAGGCAACCATGCAACCAGAGATATATAAATGACATTTCTCTG
  AAGAAGCAGGTGTTCATTGTCAGAAAGAAAAGGCTCATGGAATATCTGAATTCTGAGGAGCACAAGTGTG
  TGACCCACAAGGCGAAAAAGATGATCTTCTGTGATAAGAGCAAGATCCTCCTCTGTCACCTGTGTTCTGA
  CTCCCAGGAGCACAGCGGTCACACACACTGTTCCATTGATGTAGCTGTTCAGGAGAAAATGGAGGAACTT
  CTAAAGCACATGGACTCATTATGGCAGAGGCTCAAAATCCAGCAGAATTATGTAGAAAAAGAGAGGAGAA
  TGACCTTGTGGTGGTTGAAGTCCGTGAAGCTACGGGAGGAAGTGATCAAGAGAGTGTATGGAAAACAATG
  TCCACCCCTCAGTGAAGAAAGGGATCAACACATAGAGTGTTTGAGACATCAAAGCAACACTACTTTAGAG
  GAGCTCAGGAAAAGTGAAGCTACGATAGTCCACGAGAGAAATCAACTAACAGAGGTGTATCAGGAGCTGA
  TGACAATGTCCCAGAGGCCATACCAGGAGCTGCTGGTGCAGGACTTGGATGACTTGTTCAGAAGGAGTAA
  GCTAGCGGCAAAGGTGGACATGCCACAGGGTATGATACCAAGACTCCGTGCCCATTCCATTCCTGGGCTG
  ACTGCAAGGCTCAACTCCTTCCGAGTGAAGATTTCCTTTAAACATTCAATCATGTTCGGCTACAACTCAG
  TCAGACCTTTTGATATCAGACTTCTCCATGAAAGCACATCTCTGGATTCAGCTAAAACCCATCGTGTTTC
  CTGGGGAAAAAAGAGCTTCTCCAGGGGAAAATACTACTGGGAGGTGGATTTGAAGGACTATAGGCGGTGG
  ACTGTAGGAGTCTGTAAGGATCCCTGGTTAAGGGGGAGAAGCTATGTGGTGACACCCACAGATATATTTC
  TTCTTGAGTGTTTGAGAAACAAAGATCATTACATTCTCATCACCCGCATAGGACGTGAACACTATATAGA
  GAAGCCAGTTGGCCAGGTTGGCGTGTTCCTTGATTGTGAGGGTGGATATGTAAGTTTCGTGGATGTAGCC
  AAGAGTTCCCTCATACTCAGCTACTCTCCTGGAACTTTCCATTGTGCTGTCAGGCCTTTCTTCTTTGCTG
  CCTACACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224020 representing NM_001177858
  Red=Cloning site Green=Tags(s)
  
  MESDNLQDPQEETLTCSICQGIFMDPVYLRCGHKFCETCLLLFQEDIKFPAYCPTCRQPCNQRYINDISL
  KKQVFIVRKKRLMEYLNSEEHKCVTHKAKKMIFCDKSKILLCHLCSDSQEHSGHTHCSIDVAVQEKMEEL
  LKHMDSLWQRLKIQQNYVEKERRMTLWWLKSVKLREEVIKRVYGKQCPPLSEERDQHIECLRHQSNTTLE
  ELRKSEATIVHERNQLTEVYQELMTMSQRPYQELLVQDLDDLFRRSKLAAKVDMPQGMIPRLRAHSIPGL
  TARLNSFRVKISFKHSIMFGYNSVRPFDIRLLHESTSLDSAKTHRVSWGKKSFSRGKYYWEVDLKDYRRW
  TVGVCKDPWLRGRSYVVTPTDIFLLECLRNKDHYILITRIGREHYIEKPVGQVGVFLDCEGGYVSFVDVA
  KSSLILSYSPGTFHCAVRPFFFAAYT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177858
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177858.1, NP_001171329.1
• RefSeq Size: 1990 bp
• RefSeq ORF: 1341 bp
• Locus ID: 666731
• UniProt ID: P86449
• Cytogenetics: 9 E3.1