Ntng2 (NM_133500) Mouse Tagged ORF Clone

CAT#: MG223958

Ntng2 (tGFP-tagged) - Mouse netrin G2 (Ntng2) transcript variant a, (10ug)

Product Images

Circular map for MG223958

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ntng2
• Synonyms: 2610016D08Rik; Lmn; Lmnt2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223958 representing NM_133500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCCTGCTGGCTCTCTTCCTGCACTGCCTCCCGCTGGTCTCTGGGGACTATGACATCTGCAAAT
  CCTGGGTGACCACAGATGAGGGCCCCACCTGGGAATTCTATGCCTGCCAGCCCAAGGTGATGCGCCTGAA
  GGATTATGTCAAGGTCAAAGTGGAGCCCTCAGGCATCACGTGCGGAGACCCCCCTGAAAGGTTCTGTTCC
  CATGAGAACCCCTACCTGTGCAGTAATGAGTGTGATGCCTCCAATCCCGACCTGGCCCACCCGCCCCGGC
  TTATGTTTGACAGGGAAGATGAGGGACTGGCTACCTACTGGCAAAGCGTCACGTGGAGTCGCTACCCCAG
  TCCACTAGAGGCCAACATCACCCTCTCATGGAACAAGAGCGTGGAGTTGACAGACGACGTGGTGGTGACT
  TTTGAGTATGGCCGGCCCACGGTCATGGTCCTCGAGAAGTCCCTGGACAATGGCCGCACCTGGCAGCCCT
  ACCAGTTCTATGCAGAGGACTGCATGGAGGCCTTCGGCATGTCTGCCCGACGTGCCCGAGACATGTCACC
  CTCCAGCGCCCACCGGGTGCTCTGCACCGAGGAGTACTCACGCTGGGCAGGGTCCAAGAAAGAGAAGCAT
  GTGCGCTTTGAGGTAAGGGACCGCTTTGCCATCTTTGCCGGCCCTGACCTGCGTAACATGGACAACCTGT
  ACACGAGGATGGAGAGCGCCAAGGGCCTCAAGGAGTTCTTCACCTTCACTGACCTGCGCATGCGCCTGCT
  GCGTCCTGCGCTGGGTGGCACCTACGTGCAGCGAGAGAACCTCTACAAGTACTTCTATGCCATCTCCAAT
  ATCGAAGTCATTGGCAGGTGTAAGTGCAACCTGCATGCCAACCTGTGCACAGTGCGAGAGGGCAGCCTGC
  AGTGTGAGTGTGAACACAACACCACGGGCCCCGACTGTGGCAGGTGCAAGAAGAACTTCCGCACACGCGC
  CTGGCGAGCTGGCTCCTACCTGCCGCTGCCCCACGGCTCTCCCAATGCCTGTGCGGCCGCGGGCTCCGCC
  TTTGGCAACTGCGAGTGCTACGGCCACTCCAACCGTTGCAGCTACATTGACTTCCTGAACGTGGTGACCT
  GCGTCAGCTGTAAACACAACACTCGAGGCCAACACTGTCAGCACTGCCGCCTGGGCTACTATCGCAATGG
  CTCCGCAGAGCTGGATGATGAGAACGTCTGCATTGAATGTAACTGTAACCAGATCGGCTCTGTGCACGAT
  CGATGCAATGAGACAGGCTTCTGCGAGTGCAGGGAGGGCGCAGTGGGGCCCAAGTGCGACGACTGCCTTC
  CTACACACTACTGGCGCCAAGGATGCTATCCCAATGTGTGCGACGATGACCAGCTGCTCTGCCAGAATGG
  CGGCACCTGCCAGCAGAACCAACGCTGCGCCTGCCCGCCCGGCTACACCGGCATTCGCTGTGAGCAGCCC
  CGCTGTGACCTCGCCGACGACGCTGGCCCGGACTGTGACCGCGCGCCAGGCATCGTCCCGCGCCCCGACA
  CCCTGCTCGGATGCCTGCTGCTGCTCGGGCTGGCCGCCCGTCTGGCCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223958 representing NM_133500
  Red=Cloning site Green=Tags(s)
  
  MLRLLALFLHCLPLVSGDYDICKSWVTTDEGPTWEFYACQPKVMRLKDYVKVKVEPSGITCGDPPERFCS
  HENPYLCSNECDASNPDLAHPPRLMFDREDEGLATYWQSVTWSRYPSPLEANITLSWNKSVELTDDVVVT
  FEYGRPTVMVLEKSLDNGRTWQPYQFYAEDCMEAFGMSARRARDMSPSSAHRVLCTEEYSRWAGSKKEKH
  VRFEVRDRFAIFAGPDLRNMDNLYTRMESAKGLKEFFTFTDLRMRLLRPALGGTYVQRENLYKYFYAISN
  IEVIGRCKCNLHANLCTVREGSLQCECEHNTTGPDCGRCKKNFRTRAWRAGSYLPLPHGSPNACAAAGSA
  FGNCECYGHSNRCSYIDFLNVVTCVSCKHNTRGQHCQHCRLGYYRNGSAELDDENVCIECNCNQIGSVHD
  RCNETGFCECREGAVGPKCDDCLPTHYWRQGCYPNVCDDDQLLCQNGGTCQQNQRCACPPGYTGIRCEQP
  RCDLADDAGPDCDRAPGIVPRPDTLLGCLLLLGLAARLAC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133500
• ORF Size: 1590 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133500.2, NP_598007.1
• RefSeq Size: 1593 bp
• RefSeq ORF: 1593 bp
• Locus ID: 171171
• UniProt ID: Q8R4F1
• Cytogenetics: 2 19.57 cM
• Gene Summary: The protein encoded by this gene belongs to a subclass of the netrin family called netrin-G proteins. Unlike classic netrins, which act as diffusible chemoattractants, netrin-Gs are glycosylphosphatidylinositol-anchored membrane proteins that interact with specific transmembrane proteins. In mouse, this gene is preferentially expressed in the cerebral cortex, habenular nucleus and superior colliculus. Knockout mutant mice display a lack of behavioral startle in response to acoustic stimuli. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]