Frmpd4 (NM_001033330) Mouse Tagged ORF Clone

CAT#: MG223945

Frmpd4 (tGFP-tagged) - Mouse FERM and PDZ domain containing 4 (Frmpd4), (10ug)

Product Images

Circular map for MG223945

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Frmpd4
• Synonyms: Gm196; Pdzd10; Pdzk10; PKAP1; preso; Preso1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223945 representing NM_001033330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCACACAGCTGCCACAGGACCAAGTCTTCGGGCTGGCCACCTCCCTCAGGAACCTGGGGCTTGA
  ACCAGGTACCACCCTATGGATGGGAGATGATGACAAACCGAGATGGACGTGACTACTTCATCAATCACAT
  GACACAGGCAATCCCATTTGATGACCCTCGGTTTGACAGCTGCCAAATCATCCCCCCAGCTCCACGAAAG
  GTGGAGATGAGGAGAGACCCTGTGCTGGGCTTTGGGTTTGTGGCAGGGAGCGAAAAGCCAGTGGTCGTTC
  GGTCAGTAACACCAGGTGGCCCCTCAGAAGGCAAGCTGATCCCAGGAGATCAGATTGTAATGATTAATGA
  TGAAGCAGTCAGCGCTGCACCAAGAGAGAGAGTCATCGACCTGGTCAGGAGCTGCAAAGAATCGATTCTG
  CTCACTGTCATTCAGCCCTATCCATCTCCCAAATCAGCATTTATCAGTGCTGCTAAAAAGGCAAGATTGA
  AGTCCAATCCAGTCAAAGTGCGCTTTTCCGAAGAGGTCATAATCAATGGCCAAGTGTCGGAAACTGTTAA
  AGACAATTCACTTCTTTTTATGCCAAATGTTTTGAAAGTCTATTTGGAAAACGGACAGACCAAATCCTTT
  CGTTTTGACTGCAGCACCTCCATCAAGGATGTCATCTTAACCCTGCAAGAGAAGTTGTCTATCAAAGGCA
  TTGAACACTTCTCCCTCATGCTGGAGCAGAGAATTGAAGGGGCTGGCACCAAGCTGCTCTTGCTTCATGA
  ACAGGAGACACTCACTCAGGTGACACAGAGGCCCAGCTCCCATAAGATGAGGTGTCTTTTCCGAATCAGT
  TTTGTTCCCAAAGATCCCATTGACCTGTTAAGAAGAGATCCAGTTGCTTTTGAGTATCTCTATGTTCAGA
  GTTGTAACGATGTCGTTCAGGAGCGATTTGGACCAGAGCTGAAATATGACATTGCCTTGCGGCTGGCCGC
  GTTACAAATGTACATTGCTACTGTCACCACCAAACAGACGCAGAAAATCTCCCTCAAATACATTGAGAAA
  GAATGGGGACTAGAGACTTTTCTTCCATCTGCTGTGCTGCAGAGCATGAAAGAGAAGAACATCAAGAAAG
  CACTCTCCCACCTTGTCAAAGCAAATCAAAACTTGGTACCACCGGGTAAAAAGCTCTCTGCACTACAAGC
  TAAGGTCCACTATCTCAAGTTCCTCAGCGACCTGCGACTATATGGGGGCCGTGTATTCAAGGCAACATTA
  GTGCAGGCAGAGAAGCGCTCAGAAGTAACTCTTCTGGTTGGTCCCCGGTATGGCATAAGCCATGTCATAA
  ACACCAAAACCAATCTGGTGGCTCTTTTAGCTGACTTCAGCCATGTCAACAGGATTGAAATGTTTACTGA
  AGAGGAGAGCTTGGTGAGGGTGGAGTTGCATGTGCTAGATGTGAAGCCCATTACACTACTTATGGAGTCA
  TCAGACGCCATGAACCTGGCCTGTCTGACAGCTGGATACTACCGGCTGCTTGTGGACTCCAGGAGGTCAA
  TATTTAACATGGCCAACAAGAAAAACGCAGGCACGCAGGACACAGGATCAGAAAATAAAGGCAAGCATAA
  TCTCCTTGGTCCTGATTGGAACTGTATGCCCCAGATGACCACCTTCATTGGTGAAGGCGAACAAGAAGCC
  CAAATCACATATATAGATTCTAAGCAGAAGACAGTTGAGATGACAGACAGCACCTTGTGTCCCAAAGAGC
  ACCGGCACTTATATATCGACAACTCATACAGTTCAGATGAACTTAACCAGCCGCTGACTCAGCCAGGTGA
  TGCCCCCTGTGAGGCCGACTATAGAAGTCTAGCTCAGCGGTCCCTTTTGACCCTCTCAGGACCAGACACT
  CTGAAGAAAGCACAGGAATCTCCGCGAGGAGCTAAAGTGTCCTTTATTTTTGGAGATCTTGCCTTAGATG
  ATGGCATGAGTCCCCCAACTATAGGCTATGAAAGAATGTTAGAGGAGAATCCAGAAATGCTGGAGAAGCA
  GAGGAATCTCTACATCAGCAGTGCCAATGACATGAAGAACCTGGACCTCACTCCAGACACAGACAGCATC
  CAGTTTGTGGCAAATTCTGTATATGCAAACATAGGTGATGTGAAGAACTTTGAGGCCCCTGAGGGGATAG
  AGGAGCCCCTCTTACATGACATCTGTTATGCAGAAAATACGGACGATGCAGAAGATGAAGATGAGGTGAG
  CTGCGAGGAGGACCTCGTGGTGGGTGAAATGAACCAACCAGCCATCCTTGACCTGTCCGGGTCAAGTGAT
  GACATTATTGACCTCACAACCCTGCCTCCTCCAGAAGGAGATGACAATGAGGATGACTTCCTCCTGCGTT
  CTCTGAACATGGCCATCGCTGCTCCCCCACCTGGCTTTAGAGACAGTTCTGATGAAGAGGACACTCAGAG
  CCAGGCAACGTCCTTCCATGAGGACAAAGAACAAGGCAGCAGCCTGCAGAATGAGGAGATCCCTGTGTCC
  CTCATTGATGCTGTGCCCACCAGTGCAGAGGGCAAGTGTGAGAAGGGACTGGACCCTGCCGTCGTTTCCA
  CACTGGAAGCCCTAGAAGCTCTTTCAGAAGAACAGCAGAAGAGTGAAAATTCAGGTGTAGCCATCTTGCG
  AGCTTATAGTCCCGAGTCTTCATCAGACTCGGGCAATGAGACTAACTCTTCTGAAATGACAGAGGGTTCT
  GAGCTAGCTGCAGCACAGAAGCAGTCGGAAAGCCTCTCCCGCATGTTCTTGGCCACTCATGAAGGCTATC
  ACCCTCTGGCAGAAGAGCAGACAGAGTTCCCCACCTCCAAAGCCCCCTCAGTGGGCTTGCCTCCAAAGTC
  CTCCCATGGCCTGGCTGCTCGCCCAGCGACCGACCTCCCACCCAAAGTTGTGCCTTCCAAGCAGATCCTT
  CACTCAGACCACATGGAAATGGAGCCAGAAACCATGGAGACCAAGTCTGTCACTGACTATTTTAGCAAAC
  TGCATATGGGGTCAGTAGCATATTCCTGTACCAGCAAAAGGAAAAGCAAGCTTCCTGAGGGAGAGGGGAA
  ATCCCCCCTGAGTGGGAATATACCAGGGAAAAAACAGCAGGGAACCAAAATAGCAGAGATAGAGGAGGAC
  ACCAAAGGCAAAGCTGGCACTGTGTCTTCAAGAGACAATCCACACCTCAGCACTTTTAACCTAGAGAGAA
  CTGCCTTTCGCAAGGACAGCCAAAGATGGTATGTGGCCTCTGATGGTGGGGTGGTAGAGAAAAGTGGAAT
  GGAAGCACCAGCCATGAAAGTCTTTCCCAGAGGTCCAGGCTTGGGTAACAGAGAGGCTGAAGGGAAAGAG
  GATGGCACTGTGGAAGGAGGGGCTGATGATGCTTCAGTACTTGGCCAAGGGGACCGCTTCCTCACAGACA
  TGGCCTGTGTAGCCTCAGCCAAAGACTTAGATAACCCCGAAGATACTGACTCTCCCTCTTGTGACCATGC
  CACTAAGCTTTCTGAGGCTGAAGACAATGTGGCCCGCCTTTGTGACTACCATTTGGCCAAGCGAATGTCA
  TCCCTGCAGAGTGAGGGCCATTTTTCTCTACAGAGCTCTCAAGGCTCTTCAGTGGACACAGGCTGTGGAC
  CGGGCAGCAGTAGCAGTGCCTGTGCCACTCCTGTGGAATCGCCCCTCTGCCCGTCCATGGGGAAGCACAT
  GATTCCAGATGCTTCTGGGAAAGGAGGGAGATATATTTCACCAGAGGAGAGAGCCCCTGGCCATCCCAAC
  CATGGAGCCACCTTCGAGGAACTGCACCCACAGACAGAAGGGATGTGTCCACGCATGACAGTGCCTGCTC
  TGCACACAGCCATTAATGCCGACCCCCTGTTTGGCACTTTGAGAGATGGATGCCATCGACTGCCCAAGAT
  TAAGGAAACCACAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223945 representing NM_001033330
  Red=Cloning site Green=Tags(s)
  
  MRSHSCHRTKSSGWPPPSGTWGLNQVPPYGWEMMTNRDGRDYFINHMTQAIPFDDPRFDSCQIIPPAPRK
  VEMRRDPVLGFGFVAGSEKPVVVRSVTPGGPSEGKLIPGDQIVMINDEAVSAAPRERVIDLVRSCKESIL
  LTVIQPYPSPKSAFISAAKKARLKSNPVKVRFSEEVIINGQVSETVKDNSLLFMPNVLKVYLENGQTKSF
  RFDCSTSIKDVILTLQEKLSIKGIEHFSLMLEQRIEGAGTKLLLLHEQETLTQVTQRPSSHKMRCLFRIS
  FVPKDPIDLLRRDPVAFEYLYVQSCNDVVQERFGPELKYDIALRLAALQMYIATVTTKQTQKISLKYIEK
  EWGLETFLPSAVLQSMKEKNIKKALSHLVKANQNLVPPGKKLSALQAKVHYLKFLSDLRLYGGRVFKATL
  VQAEKRSEVTLLVGPRYGISHVINTKTNLVALLADFSHVNRIEMFTEEESLVRVELHVLDVKPITLLMES
  SDAMNLACLTAGYYRLLVDSRRSIFNMANKKNAGTQDTGSENKGKHNLLGPDWNCMPQMTTFIGEGEQEA
  QITYIDSKQKTVEMTDSTLCPKEHRHLYIDNSYSSDELNQPLTQPGDAPCEADYRSLAQRSLLTLSGPDT
  LKKAQESPRGAKVSFIFGDLALDDGMSPPTIGYERMLEENPEMLEKQRNLYISSANDMKNLDLTPDTDSI
  QFVANSVYANIGDVKNFEAPEGIEEPLLHDICYAENTDDAEDEDEVSCEEDLVVGEMNQPAILDLSGSSD
  DIIDLTTLPPPEGDDNEDDFLLRSLNMAIAAPPPGFRDSSDEEDTQSQATSFHEDKEQGSSLQNEEIPVS
  LIDAVPTSAEGKCEKGLDPAVVSTLEALEALSEEQQKSENSGVAILRAYSPESSSDSGNETNSSEMTEGS
  ELAAAQKQSESLSRMFLATHEGYHPLAEEQTEFPTSKAPSVGLPPKSSHGLAARPATDLPPKVVPSKQIL
  HSDHMEMEPETMETKSVTDYFSKLHMGSVAYSCTSKRKSKLPEGEGKSPLSGNIPGKKQQGTKIAEIEED
  TKGKAGTVSSRDNPHLSTFNLERTAFRKDSQRWYVASDGGVVEKSGMEAPAMKVFPRGPGLGNREAEGKE
  DGTVEGGADDASVLGQGDRFLTDMACVASAKDLDNPEDTDSPSCDHATKLSEAEDNVARLCDYHLAKRMS
  SLQSEGHFSLQSSQGSSVDTGCGPGSSSSACATPVESPLCPSMGKHMIPDASGKGGRYISPEERAPGHPN
  HGATFEELHPQTEGMCPRMTVPALHTAINADPLFGTLRDGCHRLPKIKETTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033330
• ORF Size: 3936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033330.4
• RefSeq Size: 8609 bp
• RefSeq ORF: 3939 bp
• Locus ID: 333605
• UniProt ID: A2AFR3
• Cytogenetics: X F5
• Gene Summary: Positive regulator of dendritic spine morphogenesis and density. Required for the maintenance of excitatory synaptic transmission. Binds phosphatidylinositol 4,5-bisphosphate.[UniProtKB/Swiss-Prot Function]