Asic5 (NM_021370) Mouse Tagged ORF Clone

CAT#: MG223851

Asic5 (tGFP-tagged) - Mouse amiloride-sensitive cation channel 5 intestinal (Accn5), (10ug)

Product Images

Circular map for MG223851

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Asic5
• Synonyms: Accn5; Blinac; Inac
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223851 representing NM_021370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACATACTGAGAAATCACAAGTGCACGCGGAGAAAGGACTCTTAGGAAAGATAAAACGTTACCTCT
  CAAAGAGACCACTGCCATCTCCAACTGACCGGAAGAAGTTCGATCAAGACTTTGCCATGTCCACTTCCTT
  TCATGGGATACATAATATTGCCCAGAACCAGAACAAAGTTCGAAAGGTGATCTGGCTGGCTGTGGTGCTG
  GGCTCTGTCTCGCTCTTGGTGTGGCAAATCTACAGTCGTTTGGTCAATTACTTCACATGGCCAACGACAA
  CATCTATAGAAGTTCAGTATGTGGAAAAAATCGAGTTCCCAGCTGTGACACTCTGTAACTTGAACAGATT
  CCAAACAGAAGCAGTATCTAGATTTGGCATCATTTTCTTCTTGTGGGACATAGTCTCCAAAGTCCTCCGC
  CTTCAGGAAATCAGTGCCAACAACACTGGCTCTCCAGAGACCTTAGATTTTGTTACGAATCACCAAAACT
  TCAGCATTACAGAGTTTGTCAAGAACAATGGTTTTTATCTCAATAACGACACTTTGGTGCACTGTGAGTT
  CTTTGGCAAAACATGCAGTCCAAAGGATTTCAAACACGTCTTTACTGAATATGGGAATTGCTTCACTTTT
  AACTACGGTGAGAATATCCAAAACAAGAATAAAGTGAGTGTGTCCGGAAGAGGCTTAAAGCTGCTCCTCG
  ACGTCCATCAGGAGGAATTCACTGACAACCCAGTCCCTGGTTTTGCTGATGCTGGTGTCATCTTTGTTAT
  TCACTCACCAAAGAAGGAGCCACAGTTTGATGGCTTAGGTTTGTCTTCTCCTGTGGGAATGCATGCACGG
  GTGACCATCCGCCAACTGAAGACGGTCCACCAGGAGTACCCATGGGGAGAATGCAATCCCAACATCAAGC
  TGAGAAACTTCATTACCTACAGCACTTACGGGTGTCTGAAGGAGTGCAAAGCCCGGCATATACAAAGGCT
  GTGTGGATGTCTGCCCTTTCTGCTTCCAGGAAATGGTGTAGAGTGTGACCTACTAGAATACTACAACTGT
  GTGTCTCCCATCCTTGATCACATCGAGCGTAAGGGACTATGCACGATGGGAACACACAACTCCAGCTGCC
  CTGTGTCATGTGAGGAAACAGAATACCCAGCTACTGTTTCTTATTCTACTTTCCCAAGTCAAAGAGCAAC
  AAGGTTTCTTGCCAAGAAATTGAATCAAAGCCAAGAATATATCCGGGAGAATCTCGTGAACATTGAAATA
  AACTATAGCGACTTAAACTATAAGATAACGCAGCAACAGAAGGCCGTGAGTGTGCCTGAGTTACTTGCAG
  ATGTTGGCGGTCAGCTGGGACTGTTCTGTGGAGCTAGTTTGATTACAATTATAGAAATTATTGAATATTT
  TTTCACCAATTTCTACTGGGTACTCATTTTCTTTTTGCTGAAAATACTTGAAACGATACAGCGGACTTCT
  CCACCTCAGGCAGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223851 representing NM_021370
  Red=Cloning site Green=Tags(s)
  
  MEHTEKSQVHAEKGLLGKIKRYLSKRPLPSPTDRKKFDQDFAMSTSFHGIHNIAQNQNKVRKVIWLAVVL
  GSVSLLVWQIYSRLVNYFTWPTTTSIEVQYVEKIEFPAVTLCNLNRFQTEAVSRFGIIFFLWDIVSKVLR
  LQEISANNTGSPETLDFVTNHQNFSITEFVKNNGFYLNNDTLVHCEFFGKTCSPKDFKHVFTEYGNCFTF
  NYGENIQNKNKVSVSGRGLKLLLDVHQEEFTDNPVPGFADAGVIFVIHSPKKEPQFDGLGLSSPVGMHAR
  VTIRQLKTVHQEYPWGECNPNIKLRNFITYSTYGCLKECKARHIQRLCGCLPFLLPGNGVECDLLEYYNC
  VSPILDHIERKGLCTMGTHNSSCPVSCEETEYPATVSYSTFPSQRATRFLAKKLNQSQEYIRENLVNIEI
  NYSDLNYKITQQQKAVSVPELLADVGGQLGLFCGASLITIIEIIEYFFTNFYWVLIFFLLKILETIQRTS
  PPQAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021370
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021370.2, NP_067345.1
• RefSeq Size: 1657 bp
• RefSeq ORF: 1488 bp
• Locus ID: 58170
• UniProt ID: Q9R0Y1
• Cytogenetics: 3 E3
• Gene Summary: Cation channel that gives rise to very low constitutive currents in the absence of activation. The activated channel exhibits selectivity for sodium and lithium, and is inhibited by amiloride (By similarity).[UniProtKB/Swiss-Prot Function]