Prss36 (NM_001081374) Mouse Tagged ORF Clone

CAT#: MG223812

Prss36 (tGFP-tagged) - Mouse protease serine 36 (Prss36), (10ug)

Product Images

Circular map for MG223812

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prss36
• Synonyms: C330007D15Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223812 representing NM_001081374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCATCACCTGTTTCTCCCAGTTGTAATCATGGTGGTCAGTCCCATCCCTCCAGGAGCCTTCCAGG
  ACTCAGTGGTCAGTCCTACCCAAGGAGAATTTGAAGACCTAGACTGCGGCCGCCCTGAGCCCTCTTCCCG
  CATTGTGGGGGGCTCGGACGCTCATCCTGGCACTTGGCCATGGCAGGTGAGCCTGCATCAAGGTGGGGGC
  CACATCTGCGGGGGCTCCCTCATCGCCCCTTCCTGGGTCCTCTCCGCTGCTCACTGTTTCGTGACGAATG
  GAACCTTGGAGCCCGCGGACGAATTGTCAGTTCTGCTGGGCGTGCACTCCCAGGACGGGCCCCTGGAAGG
  AGCGCACATGCGCTCAGTGGCCACCATCCTGATACCGGACAACTACAGCACAGTGGAACTGGGCGCAGAC
  TTGGCTCTGCTGCGCCTGGCCTCGCCAGCCAAGCTGGGCCCCTCTGTGCGGCCGGTCTGCCTGCCTCGTG
  CCTCGCACCTCTTCGCTCACGGAACTGCCTGCTGGGCCACTGGTTGGGGGGACGTCCAGGAGGCTGTCCC
  TCTGCCTCTGCCCTGGGTTCTACAGGAAGTGGAGCTAAGGTTGCTGGGAGAGGCTGCCTGTCAATGTCTC
  TACAGCCGTCCTGGCCCTTTCAACCTGACTTTCCAGCTGTTGCCAGGGATGTTGTGTGCTGGCTATCCAG
  CGGGACGAAGGGATACCTGCCAGGGTGACTCTGGGGGACCGCTGGTCTGTGAGGACGGAGGCCGATGGTT
  CCTGGCCGGAATCACCAGCTTTGGCTTTGGCTGTGGACGGAGGAACCGCCCTGGGGTCTTCACTGCAGTG
  GCTCCCTATGAGTCGTGGATTCGGGAACACGTGATGGGTTCAGAACCTGGGCCTGTCTTTCCCAGCCAGC
  TCCAGAAGCCACAGTCAGGACCCTGGGAGCCCAGAGAAGAAAACTGTACCTTTGCTCAACCAGAGTGCGG
  GAAGGCTCCGAGGCCGGGGACCTGGCCCTGGGAGGCCCAAGTGACCGTACCAGGCTCCACACCCTGCTAT
  GGAGCTCTGGTGTCTGACAGATGGGTCTTGGCACCCGCCAGCTGCTTTCTGGACTTCCCCACCCAGCAGC
  CCCGCACCTCTGACAGCTCACCCCACGATTTTGAGACCTGGCGGGTGCTGCTGCCCTCCCGTCCGGAGGA
  GGAGAGGGTGGCGCGCCTCGTGGCGCACGAGAACGCTTCCAGGGACTTCGCTTCGGACCTGGCGCTGCTG
  CAGCTGAGGACGCGCGTGAACCTGACCGCGGCCCCGAGTGCCGTGTGCCTGCCCCACCACGAACACTATT
  TTCTGCCCGGGAGCCACTGCCGCCTGGCTCGCTGGGGACGCGGGGAACTCGCCCCGGGCTCCAGCGCGCA
  GCTGGAGGCGCAGCTGTTGAACGGCTGGTGGTGTCATTGCCTGTACGGCCGCCAGGGGGAGACCGTGCCG
  CGGCCAGGAGACCCGCCGCACTTGCTTTGTCCCGCCTACCAGGAGGAGGAAGAGGCGGGCCTATGTTGGA
  AAGATTCCAGTTGGAGCCTTCTGTGCCGTGAGGAGGGGACCTGGTTCCTGGCAGGATACAGAACCCTCTC
  AGATGGTTGTCTCCGACCCCGAGCCTTTTCCCCAATGCAGACTCATGGTCCGTGGATCCGTCATGTGACT
  CAGGGAGCTTACCTGGAGGACCAGCTAACCTGGGACTGGGGCCCTGAAGGGGAGGAGACCGAGAAACAGA
  CTTGTCCCACACATACTGAGCACGGTGCCTGTGGCCTAAGGCCAAAATCCACTCCAGCTGGTGTGTTGTG
  GCCCTGGCTGACGGAGGTGCATGTGACTGGTGATAGAGTCTGCACTGGGATTCTGGTGGCCCCAGGCTGG
  GTCCTAGCAGCCACTCACTGTATTCTCAGGCTGGGCTCTACAACAGTGCCCTACATTGAAGTATATCTTG
  GCCGGGCAGGGGTCAGCTCCCTCCCACAGGGCCACCAGGTATCTCGGTCTGTCGTCAGCATCCGTCTGCC
  CCGGCACTTAGGACTTAGACCCCCCCTGGCTCTACTGGAGCTGAACTCTCGGGTGGAGCCCTCCCCATCA
  GCTCTTCCCATCTGCCTACACCCAGGGGGTATTCCTTCAGGGGCCAGCTGCTGGGTTTTGGGCTGGAAGA
  ACCCACAGGACAGAGTTCCTGTAGTTGCTGCTGTCTCCATCTTGACACCACGACTCTGTCACTGCCTCTA
  TCCGGGTATTCTGACTCCTGGGACCTTCTGTGTCCTCTATTCAGAGGGACAGGAGGATAGATGTGAGGTC
  ACCTCAGCACCTCCCCTCCTGTGCCAGACTGAGGAAGGTCCCTGGGTTCTCGTGGGCATGGCTGTTCGAG
  GAAACCGGGAGCTGTTTGCAGCCATTGGCCCTGAAGCTACATGGATATCGCAAACCGTGGGAGAAGCCCA
  TTTTCTTCATCTCGGTGGCTCCTCCTACTGGTCTCCTGAAGGCAGTGACCTCTGCCCCCAAGACTTAGCA
  GGGTCTGCTAGCTCCCCTAAGGTCACTGCTCTGCTTCTGCTCCTCCTCCTGGCTCCACTGATCCCTCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223812 representing NM_001081374
  Red=Cloning site Green=Tags(s)
  
  MSHHLFLPVVIMVVSPIPPGAFQDSVVSPTQGEFEDLDCGRPEPSSRIVGGSDAHPGTWPWQVSLHQGGG
  HICGGSLIAPSWVLSAAHCFVTNGTLEPADELSVLLGVHSQDGPLEGAHMRSVATILIPDNYSTVELGAD
  LALLRLASPAKLGPSVRPVCLPRASHLFAHGTACWATGWGDVQEAVPLPLPWVLQEVELRLLGEAACQCL
  YSRPGPFNLTFQLLPGMLCAGYPAGRRDTCQGDSGGPLVCEDGGRWFLAGITSFGFGCGRRNRPGVFTAV
  APYESWIREHVMGSEPGPVFPSQLQKPQSGPWEPREENCTFAQPECGKAPRPGTWPWEAQVTVPGSTPCY
  GALVSDRWVLAPASCFLDFPTQQPRTSDSSPHDFETWRVLLPSRPEEERVARLVAHENASRDFASDLALL
  QLRTRVNLTAAPSAVCLPHHEHYFLPGSHCRLARWGRGELAPGSSAQLEAQLLNGWWCHCLYGRQGETVP
  RPGDPPHLLCPAYQEEEEAGLCWKDSSWSLLCREEGTWFLAGYRTLSDGCLRPRAFSPMQTHGPWIRHVT
  QGAYLEDQLTWDWGPEGEETEKQTCPTHTEHGACGLRPKSTPAGVLWPWLTEVHVTGDRVCTGILVAPGW
  VLAATHCILRLGSTTVPYIEVYLGRAGVSSLPQGHQVSRSVVSIRLPRHLGLRPPLALLELNSRVEPSPS
  ALPICLHPGGIPSGASCWVLGWKNPQDRVPVVAAVSILTPRLCHCLYPGILTPGTFCVLYSEGQEDRCEV
  TSAPPLLCQTEEGPWVLVGMAVRGNRELFAAIGPEATWISQTVGEAHFLHLGGSSYWSPEGSDLCPQDLA
  GSASSPKVTALLLLLLLAPLIPR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081374
• ORF Size: 2589 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081374.1, NP_001074843.2
• RefSeq Size: 3061 bp
• RefSeq ORF: 2592 bp
• Locus ID: 77613
• Cytogenetics: 7 F3