Rprd2 (NM_001081293) Mouse Tagged ORF Clone

CAT#: MG223708

Rprd2 (tGFP-tagged) - Mouse regulation of nuclear pre-mRNA domain containing 2 (Rprd2), (10ug)

Product Images

Circular map for MG223708

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rprd2
• Synonyms: 2810036A19Rik; 4930535B03Rik; 6720469I21Rik; AL022841; AL022940; AU021304; BB077382
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223708 representing NM_001081293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGGCGGCGGCGGAGGCAGCAGTAAGGCCTCCTCCTCGTCGGCCTCGTCCGCAGGGGCCCTGG
  AGTCTTCGTTGGATCGAAAATTCCAGTCGGTGACCAACACCATGGAATCCATTCAAGGCTTGTCGTCCTG
  GTGTATAGAAAACAAGAAACACCACAGTACAATCGTCTACCATTGGATGAAGTGGCTCCGGAGATCTACA
  TATCCCCATCGTTTGAATCTCTTTTATCTTGCCAATGATGTCATACAGAATTGTAAACGGAAAAATGCAA
  TCATATTTCGTGAATCATTTGCTGATGTGCTTCCTGAAGCAGCTGCTCTAGTGAAGGATCCATCCGTCTC
  TAAGTCTATAGAACGAATCTTTAAAATCTGGGAAGATCGAAATGTGTACCCAGAAGACATGATTGTGGCA
  TTGAGAGAAGCTTTAATGGACAGAGCAGCTTCCCATAATGCCAGACTGCAAAAACTGCAATGTTTTCCAG
  GTACCACTTTCAAAACTCAGAAGCAGCTGAAAGAAAATCTGAACAAACAACCGAATAAGCAGTGGAAGAA
  ATCACAAACATCCACGAATCCAAAAGCTGCTCTCAAATCTAAGATTGTTGCTGAGTTTCGATCTCAGGCC
  CTCATTGAAGAGTTGCTGATGTACAAACGATCAGAAGATCAGATAGAACTGAAGGAGAAACAGTTGTCAA
  CTATGAGAGTGGATGTCTGCAGCACAGAAACTCTCAAGTGTTTAAAAGATAAAACAGGTGGAAAGAAGTT
  CTCCAAAGAATTTGAAGAAGCAAGTTCCAAGCTAGAGGAGTTTGTGAATGGATTAGATAAACAGGTGAAA
  AATGGGCCCTCACTAACAGAAGCATTAGAGAACGCTGGAATTTTCTATGAAGCACAGTACAAGGAAGTGA
  AAGTGGTCGCTAACGCTTACAAAACCTTCGCTAACCGAGTAAACAACTTAAAGAAGAAGTTGGATCAATT
  GAAGTCAACCCTTCCTGATCCTGAGGAATCACCAGTCCCCTCTCCAAGTATGGATGCTCCTTCCCCAACT
  GGTTCTGAGTCTCCATTCCAGGGCATGGGAGGTGAAGAACCCCAGTCACCGGCCATGGAGAGTGACAAGT
  CTGCCACACCCGAGCCTGTGACTGATAACCGTGATGTGGAAGACATGGAACTCTCAGATGTGGAAGATGA
  TGGATCAAAAATCATTGTGGAGGACCGGAAAGAAAAGCCTGTGGAGAAACCTGCTGTCTCCACTGGTGTG
  CCCACCAAGTCAACAGAAAGTGTCTCGAAAGCCTCACCGTGTGCCCCACCGTCTGTGCCCACGACGGCAG
  CTCCACCTCTCCCGAAGCCTCTGAGCACTGCACTTCTGTCCCCTTCCCCAACTTTGGTTTTGCCAAACCT
  GGCAAATGTGGATCTGGCAAAGATCAGTTCCATCCTTAGCAGCCTAACATCAGTCATGAAAAATACGGGG
  GTCAGTTCTGCATCAAGACCTTCTCCAGGAATTCCTACCAGTCCCAGCAACCTCAGCAGTGGCCTGAAAA
  CACCTGCACCTGCCACAACACCATCTCACAACCCTCTGGCAAATATCCTGTCAAAGGTGGAGATCACCCC
  AGAGAGCATTCTCTCTGCTCTTTCTAAAACCCAGACACAGTCAGCCCCTGCACTCCAAGGCTTGTCATCA
  TTACTTCAGAGTGTTACTGCGAACCCTGTGCCAGCCAGTGAAGTCACTTCCCAGAGCACCACAGCCTCCC
  CTGCGAGCACCACAGGCTCTGCCGTAAAGGGGAGAAATCTACTCTCCAGTACCCAGTCTTTTATTCCCAA
  AAGCTTCAACTATTCTCCTAGTTCTTCAACTTCTGAAGTCTCTTCCACTTCAGCAAGCAAGGCGTCAGTT
  GGGCAGAGCCCGGTGCTCCCAAGCACTACGTTTAAACTACCATCCAGTTCTTTGGGATTTACTGGTACCC
  ACAATCCTAGCCCTGCTGCCCCACCTACTGAAGTTGCCGTGTGCCAATCCTCAGAGGTCTCCAAGCCAAA
  GCCAGAATCAGAGTCCACCTCTCCAAGCCTGGAAATGAAGATCCACAACTTCTTGAAAGGTAATCCTGGT
  TTCAGTGGCTTAAACTTAAACATCCCGATCCTAAGCAGCTTGGGATCCAGTGCCCCATCAGAGGGCCATG
  CCTCAGACTTCCAGCGTGGTCCTACTAGCACTTCAGTTGACAGCATCGATGGAACTCCTGTTCGGGATGA
  ACGGAGTGGGACACCCACCCAGGATGAGATGATGGATAAGCCCACATCCAGCAGCGTAGACACTATGTCC
  CTGCTGTCTAAGATCATCAGCCCTGGTTCCTCAACACCCAGCAGTACAAGGTCGCCACCTCCAGGAAGAG
  ATGAAAGCTACCCCCAAGAGCTCCCCAATTCTGTGTCTACATATCGACCGTTTGGTCTGGGCAGTGACTC
  TCCCTACAAGCAGCCTTCTGGTGGAGTTGAGAGGCCGTCATCACTGATGGACTCTTCACAGGAAAAGCTC
  TTCCCAGATACTTCCTTCCAGGAAGACGAGGACTACCGAGATTTTGAGTATTCAGGGCCTCCTCCATCTG
  CCATGATGAATCTAGAGAAGAAACCAGCCAAATCCATCCTGAAGTCAAGCAAGCTTTCTGATGCCACTGA
  GTACCAGCCAATCCTGTCTAGTTACAACCACAGGGCCCAAGAGTTTGGGGTCAAGTCTGCCTTCCCTCCA
  TCTGTAAGGGCCCTCCTGGACTCTAGTGAGAACTGTGACCGTCTCTCATCTCCCCCTGGGCTATTTGGTG
  CCTTCAACATAAGAGGGAATGAACCTGGGTCTGAGCGGTCACCATCACCAAGTAAAAATGATGCATTTTT
  CACCCCTGACTCCAACCACAGTGGCTTGTCTCAGTCTACTGCTGGGCATCTCACGTTGCCACAGACACAA
  TACCCAGACTCTCCTCACTCAGTCCCACATCGTTCCATTTTCTCTTCACAGAGTACCCTTGCCGCTCCAG
  CAGGCCACCCACCCACATCAGGCGTGGAAAAAGTCCTGGCCTCCACGATTTCCACCACATCGACGATTGA
  GTTTAAGAATATGCTTAAAAACGCCTCACGCAAGCCCTCAGATGATAAGCATTTTGGCCAGACACCCAAC
  AAGGGTACTTCAAGTGATGGTGTCAGTCTCTCAAACCTCACCCAGCCCAGCTTGCCCACCACTGACCAAC
  AACAGGAAGAACACTATCGAATAGAAACTCGTGTTTCCTCCTCCTGTTTAGACTTGCCTGACAGCACAGA
  AGAAAAGGGGGCCCCTATAGAAACTCTAGGTTATCATAACGCAGCTAATAGGAGGATGTCAGGGGAGCCG
  ATAAAGACTGTAGAGTCTATACGAGTTCCTGGGAAGGGAAATAGAGGACATGGGCGCGAGGTTTCAAGAG
  TGGGTTGGTTTGATCTGAGCACACCAGGCAGCTCTTTTGACAATGGTCCCTCAAGTGCCTCTGAGTTGGC
  ATCCCTTGGGGGTGGGGGCAGCGGAGGCCTCACTGGCTTTAAGACAACGCCGTACAAGGAACGGGCACCC
  CAGTTTCAGGAGAGTGTCACCAGCTTTCGTTCCAACAGTTTCAACTCAACATTTGAGCATCATCTCCCCC
  CATCCCCTTTGGAACATGGGGCACCCTTCCAGAGAGAGCCAGTGGGGCCGTCATCTGCCCCACCTGCCCC
  TCCTAAGGATCATGGTGGTATCTTCTCTCGAGAGGCACCCACTCATCTGCCCTCTGTGGATCTTTCGAAC
  CCCTTTACAAAGGAGGCATCTCTGGCCCACGCTGGCCCACCACCTCCTCCAGGAGAGCACAGCGGAGTTC
  CTTTCCCTCCACCCCCCCCTCCTCCACCCCCGGGGGAACTTAGCAGTGGAGGGACTGGTGTCCCCTTTGC
  TACTCCAGCCCCGCCTCCACCCCCTGTTGACCACTCTGGGGTTGTACCTTTCCCAACTCCACCACTGCCA
  GAGCATGGAGTAACTGGGGCTGTGTCAGTTTTTCCCAAGGACCATAGTTCCCTCCTTCAAGGGACCATGG
  CTGAGCATTTTGGGGTGCTCACAGGACCCAGGGACCTCAATGGCCCTGGTCTTAACCGTTCACGAGAGAG
  CCTGAGCCTGCCTTCCCATCCTCTGGAGCACCTGGGCCCAGCCCTTGGAGGAGGTGGGGGAGGCAACACC
  AGCAGCAGTGGCCTTCCCTTGAGTCCCGCACACAGAGACGCCATCGGCCGGAGTGGTATGATTTTACGGA
  GTCCCCGGCCAGACTTTCGGCCTAGGGAAGCTTTTCTCGGCAGAGACCCATTCCACAGTTTAAAGAGACC
  CAGGCCACCTTTTGTTAGAGGCCCTCCGTTCTTTGCACCAAAACGCCCATTTTTCCCTCCCAGGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223708 representing NM_001081293
  Red=Cloning site Green=Tags(s)
  
  MAAGGGGGSSKASSSSASSAGALESSLDRKFQSVTNTMESIQGLSSWCIENKKHHSTIVYHWMKWLRRST
  YPHRLNLFYLANDVIQNCKRKNAIIFRESFADVLPEAAALVKDPSVSKSIERIFKIWEDRNVYPEDMIVA
  LREALMDRAASHNARLQKLQCFPGTTFKTQKQLKENLNKQPNKQWKKSQTSTNPKAALKSKIVAEFRSQA
  LIEELLMYKRSEDQIELKEKQLSTMRVDVCSTETLKCLKDKTGGKKFSKEFEEASSKLEEFVNGLDKQVK
  NGPSLTEALENAGIFYEAQYKEVKVVANAYKTFANRVNNLKKKLDQLKSTLPDPEESPVPSPSMDAPSPT
  GSESPFQGMGGEEPQSPAMESDKSATPEPVTDNRDVEDMELSDVEDDGSKIIVEDRKEKPVEKPAVSTGV
  PTKSTESVSKASPCAPPSVPTTAAPPLPKPLSTALLSPSPTLVLPNLANVDLAKISSILSSLTSVMKNTG
  VSSASRPSPGIPTSPSNLSSGLKTPAPATTPSHNPLANILSKVEITPESILSALSKTQTQSAPALQGLSS
  LLQSVTANPVPASEVTSQSTTASPASTTGSAVKGRNLLSSTQSFIPKSFNYSPSSSTSEVSSTSASKASV
  GQSPVLPSTTFKLPSSSLGFTGTHNPSPAAPPTEVAVCQSSEVSKPKPESESTSPSLEMKIHNFLKGNPG
  FSGLNLNIPILSSLGSSAPSEGHASDFQRGPTSTSVDSIDGTPVRDERSGTPTQDEMMDKPTSSSVDTMS
  LLSKIISPGSSTPSSTRSPPPGRDESYPQELPNSVSTYRPFGLGSDSPYKQPSGGVERPSSLMDSSQEKL
  FPDTSFQEDEDYRDFEYSGPPPSAMMNLEKKPAKSILKSSKLSDATEYQPILSSYNHRAQEFGVKSAFPP
  SVRALLDSSENCDRLSSPPGLFGAFNIRGNEPGSERSPSPSKNDAFFTPDSNHSGLSQSTAGHLTLPQTQ
  YPDSPHSVPHRSIFSSQSTLAAPAGHPPTSGVEKVLASTISTTSTIEFKNMLKNASRKPSDDKHFGQTPN
  KGTSSDGVSLSNLTQPSLPTTDQQQEEHYRIETRVSSSCLDLPDSTEEKGAPIETLGYHNAANRRMSGEP
  IKTVESIRVPGKGNRGHGREVSRVGWFDLSTPGSSFDNGPSSASELASLGGGGSGGLTGFKTTPYKERAP
  QFQESVTSFRSNSFNSTFEHHLPPSPLEHGAPFQREPVGPSSAPPAPPKDHGGIFSREAPTHLPSVDLSN
  PFTKEASLAHAGPPPPPGEHSGVPFPPPPPPPPPGELSSGGTGVPFATPAPPPPPVDHSGVVPFPTPPLP
  EHGVTGAVSVFPKDHSSLLQGTMAEHFGVLTGPRDLNGPGLNRSRESLSLPSHPLEHLGPALGGGGGGNT
  SSSGLPLSPAHRDAIGRSGMILRSPRPDFRPREAFLGRDPFHSLKRPRPPFVRGPPFFAPKRPFFPPRY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081293
• ORF Size: 4407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081293.1, NP_001074762.1
• RefSeq Size: 8419 bp
• RefSeq ORF: 4410 bp
• Locus ID: 75137
• UniProt ID: Q6NXI6
• Cytogenetics: 3 F2.1