Ptpru (NM_011214) Mouse Tagged ORF Clone

CAT#: MG223553

Ptpru (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type U (Ptpru) transcript variant 1, (10ug)

Product Images

Circular map for MG223553

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptpru
• Synonyms: Ftp-1; PCP-2; Pcp2; PTP; PTP-lambda; Ptpf; PTPlambda; Ptprl; R-PTP-psi; R-PTP-U; RPTPlambda
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223553 representing NM_011214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGCTCAGGCTCTGGTCCTGGCGCTCACCTTCCAGTTCTGCGCGCCTGAGACCGAGACTCCCG
  CAGCTGGCTGCACCTTCGAGGAGGCGAGTGACCCGGTCGTGCCCTGCGAGTTCAGCCAGGCTCAGTATGA
  CGACTTCCAATGGGAGCAAGTGCGGATCCACCCCGGCACCCGGACCCCTGAAGACCTGCCCCATGGTGCC
  TACTTGATGGTCAATGCTTCTCAGCATGCCCCAGGTCAGAGGGCCCACATCATCTTCCAGACCCTGAGCG
  AGAACGACACCCATTGTGTGCAGTTCAGCTACTTCCTGTACAGCAGGGATGGGCACAGCCCAGGCACCCT
  GGGGGTCTACGTGCGCGTGAATGGGGGCCCTCTGGGCAGTGCCGTGTGGAATATGACCGGATCCCACGGC
  CGTCAGTGGCACCAGGCTGAGCTGGCTGTCAGCACCTTCTGGCCCAATGAGTATCAGGTGCTGTTTGAGG
  CCCTCATCTCCCCAGACCACAAGGGCTACATAGGCTTAGACGACATCTTGCTCTTCAGCTATCCCTGCGC
  AAAGGCCCCTCACTTCTCCCGCCTTGGGGACGTGGAGGTCAATGCAGGCCAGAACGCATCCTTCCAATGC
  ATGGCAGCAGGCAGAGCCGCAGAGGCAGAACACTTCTTCCTGCAGCGTCAGAGTGGAGTGCTGGTGCCTG
  CGGCCGGGGTGCGGCACATCAGCCACCGTCGCTTCCTGGCCACTTTTCCGCTGGCCTCGGTAGGCCGCTC
  AGAGCAGGATCTGTACCGTTGCGTGTCCCAGGCCCCGCGTGGTGCTGGCGTCTCCAACTTTGCAGAGCTC
  ATCGTCAAAGAGCCTCCCACCCCCATCGCGCCCCCACAGCTGCTGCGTGCAGGCCCCACCTACCTCATTA
  TCCAGCTCAACACCAACTCCATCATTGGCGACGGGCCGATCGTGCGCAAGGAGATCGAGTACCGCATGGC
  ACGGGGCCCGTGGGCCGAGGTGCACGCTGTCAACCTGCAGACCTACAAGCTGTGGCATCTGGACCCAGAC
  ACTGAGTATGAAATCAGCGTGCTGCTCACACGCCCGGGAGATGGAGGCACAGGCCGCCCTGGGCCACCAC
  TGATCAGCCGGACCAAGTGCGCAGAGCCCACGAGGGCCCCCAAAGGTCTGGCTTTTGCTGAGATCCAGGC
  TCGCCAGCTGACCCTGCAGTGGGAGCCCCTGGGCTATAATGTCACACGTTGTCATACCTACGCTGTGTCC
  CTTTGCTATCGCTACACCCTGGGCGGCAGCCACAACCAGACCATCCGGGAGTGTGTGAAGATGGAGCGGG
  GTGCCAGCCGCTACACCATCAAGAATCTGCTGCCATTCAGAAACATCCACGTGCGTCTGATTCTCACAAA
  CCCTGAGGGGCGCAAGGAGGGCAAGGAGGTCACCTTCCAGACAGATGAAGATGTGCCTGGTGGGATTGCA
  GCTGAGTCCCTAACCTTCACTCCACTGGAGGACATGATCTTTCTCAAGTGGGAGGAGCCCCAGGAGCCCA
  ATGGCCTCATCACTCAGTATGAGATCAGCTACCAAAGCATTGAGTCCTCAGACCCAGCAGTGAACGTGCC
  CGGCCCGAGACGCACCATCTCCAAACTCCGGAATGAGACTTACCACGTCTTCTCCAACCTGCATCCCGGC
  ACCACGTATCTGTTCTCCGTGCGTGCTCGGACGAGCAAGGGCTTCGGCCAGGCGGCTCTCACTGAGATAA
  CCACCAACATCTCAGCTCCCAGCTTTGATTATGCCGACATGCCGTCACCCCTGGGCGAGTCCGAGAACAC
  CATCACTGTGCTGTTGAGGCCGGCCCAGGGCCGAGGAGCCCCCATCAGCGTCTACCAGGTGGTTGTGGAG
  GAAGAGCGGCCACGGCGCTTGCGGCGGGAGCCCGGAGCTCAGGACTGCTTCTCGGTACCTCTGACCTTTG
  AGACGGCCCTGGCTCGCGGCCTGGTGCACTACTTTGGGGCTGAACTGGCTGCCAGCAGCCTGCTTGAGGC
  CATGCCCTTCACCGTGGGTGACAACCAGACCTATCGTGGCTTCTGGAACCCACCGCTTGAGCCCAGAAAG
  GCCTATCTCATCTATTTCCAGGCAGCAAGCCACCTGAAAGGGGAAACCCGACTGAACTGCATCCGAATTG
  CCAGGAAAGCTGCGTGCAAGGAGAGCAAGCGACCCCTCGAAGTGTCCCAGAGATCGGAGGAGATGGGGCT
  CATCCTGGGCATCTGTGCAGGTGGTCTTGCCGTCCTCATTCTCCTCCTGGGGGCCATCATTGTCATCATC
  CGCAAAGGGAAGCCAGTGAACATGACGAAAGCCACGGTCAACTACCGCCAGGAGAAGACTCACATGATGA
  GTGCCGTGGACCGCAGCTTCACAGATCAGAGTACTCTGCAGGAGGATGAGCGGTTGGGTCTGTCCTTTAT
  GGATGCTCCTGGCTATAGTCCTCGTGGAGACCAGCGAAGCGGTGGTGTCACCGAGGCCAGCAGCCTCCTG
  GGGGGTTCTCCAAGGCGCCCATGCGGCCGGAAGGGTTCTCCGTATCATACCGGGCAGCTCCACCCTGCAG
  TCCGAGTGGCTGACCTTCTACAGCACATCAACCAGATGAAGACAGCCGAGGGCTACGGCTTCAAGCAGGA
  GTACGAGAGTTTCTTTGAGGGCTGGGACGCCACCAAGAAGAAAGACAAGCTCAAGGGCGGCCGACAGGAG
  CCAGTGTCTGCCTATGATCGACACCATGTGAAACTACACCCGATGCTGGCAGACCCTGATGCCGACTACA
  TCTCTGCCAACTACATAGACGGCTACCACAGGTCAAACCACTTCATAGCCACTCAAGGGCCAAAGCCTGA
  GATGATCTACGATTTCTGGCGCATGGTGTGGCAGGAACAGTGTGCGAGCATCGTCATGATCACCAAGCTG
  GTAGAGGTGGGCAGGGTGAAGTGTTCTCGCTACTGGCCTGAGGACTCAGACATGTATGGGGACATCAAGA
  TCACGCTGGTAAAGACAGAGACACTGGCTGAGTATGTGGTGCGCACCTTTGCCCTGGAGCGGAGAGGTTA
  CTCAGCCCGGCATGAGGTCCGCCAGTTCCATTTCACAGCGTGGCCAGAGCATGGTGTCCCCTACCACGCC
  ACGGGGCTGCTGGCCTTCATCCGGCGTGTGAAGGCTTCCACTCCACCTGATGCCGGGCCCATTGTCATTC
  ACTGCAGTGCAGGAACTGGCCGCACAGGCTGCTACATCGTCCTGGATGTGATGCTGGACATGGCTGAATG
  TGAGGGGGTCGTGGACATTTACAACTGTGTGAAGACCCTCTGTTCCCGACGGGTCAACATGATCCAGACG
  GAGGAACAATATATCTTCATCCACGATGCAATCTTGGAGGCCTGCCTGTGTGGGGAGACCACCATCCCTG
  TCAACGAGTTCAAGGCCACCTACAGGGAGATGATCCGCATTGACCCTCAGAGCAATTCCTCCCAGCTTCG
  GGAAGAGTTCCAGACGCTGAACTCGGTCACGCCGCCGCTGGATGTGGAGGAGTGTAGCATTGCCCTGCTG
  CCCCGGAATCGAGACAAGAACCGTAGCATGGATGTGCTGCCACCAGACCGCTGCCTGCCCTTCCTCATCT
  CCAGTGATGGGGACCCCAATAACTACATCAATGCAGCACTGACTGACAGCTACACACGGAGCGCCGCCTT
  CATCGTGACCCTGCACCCGCTGCAGAGTACCACGCCCGACTTCTGGCGGCTGGTCTACGACTACGGGTGC
  ACCTCCATCGTCATGCTGAACCAACTTAACCAGTCCAACTCCGCCTGGCCCTGCTTGCAGTACTGGCCGG
  AGCCAGGCCGACAGCAGTATGGGCTCATGGAGGTGGAGTTTGTGTCTGGCACAGCAAACGAGGATTTGGT
  GTCCCGAGTGTTCCGGGTGCAGAACTCTTCTCGGCTGCAGGAGGGTCACCTGCTGGTACGGCACTTCCAG
  TTTCTGCGTTGGTCTGCTTATCGGGACACGCCTGACTCCAGGAAGGCCTTTCTGCACCTGTTGGCTGAGG
  TGGACAAGTGGCAGGCAGAGAGTGGGGATGGGCGCACCGTGGTGCATTGTCTCAACGGGGGTGGCCGCAG
  TGGCACCTTCTGCGCCTGTGCCACGGTCTTGGAGATGATCCGCTGTCACAGCCTGGTGGATGTTTTCTTT
  GCTGCCAAAACACTTCGGAACTACAAGCCCAATATGGTGGAGACCATGGATCAGTATCATTTCTGCTACG
  ACGTGGCCCTGGAGTACCTGGAGGCTCTGGAGTTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223553 representing NM_011214
  Red=Cloning site Green=Tags(s)
  
  MARAQALVLALTFQFCAPETETPAAGCTFEEASDPVVPCEFSQAQYDDFQWEQVRIHPGTRTPEDLPHGA
  YLMVNASQHAPGQRAHIIFQTLSENDTHCVQFSYFLYSRDGHSPGTLGVYVRVNGGPLGSAVWNMTGSHG
  RQWHQAELAVSTFWPNEYQVLFEALISPDHKGYIGLDDILLFSYPCAKAPHFSRLGDVEVNAGQNASFQC
  MAAGRAAEAEHFFLQRQSGVLVPAAGVRHISHRRFLATFPLASVGRSEQDLYRCVSQAPRGAGVSNFAEL
  IVKEPPTPIAPPQLLRAGPTYLIIQLNTNSIIGDGPIVRKEIEYRMARGPWAEVHAVNLQTYKLWHLDPD
  TEYEISVLLTRPGDGGTGRPGPPLISRTKCAEPTRAPKGLAFAEIQARQLTLQWEPLGYNVTRCHTYAVS
  LCYRYTLGGSHNQTIRECVKMERGASRYTIKNLLPFRNIHVRLILTNPEGRKEGKEVTFQTDEDVPGGIA
  AESLTFTPLEDMIFLKWEEPQEPNGLITQYEISYQSIESSDPAVNVPGPRRTISKLRNETYHVFSNLHPG
  TTYLFSVRARTSKGFGQAALTEITTNISAPSFDYADMPSPLGESENTITVLLRPAQGRGAPISVYQVVVE
  EERPRRLRREPGAQDCFSVPLTFETALARGLVHYFGAELAASSLLEAMPFTVGDNQTYRGFWNPPLEPRK
  AYLIYFQAASHLKGETRLNCIRIARKAACKESKRPLEVSQRSEEMGLILGICAGGLAVLILLLGAIIVII
  RKGKPVNMTKATVNYRQEKTHMMSAVDRSFTDQSTLQEDERLGLSFMDAPGYSPRGDQRSGGVTEASSLL
  GGSPRRPCGRKGSPYHTGQLHPAVRVADLLQHINQMKTAEGYGFKQEYESFFEGWDATKKKDKLKGGRQE
  PVSAYDRHHVKLHPMLADPDADYISANYIDGYHRSNHFIATQGPKPEMIYDFWRMVWQEQCASIVMITKL
  VEVGRVKCSRYWPEDSDMYGDIKITLVKTETLAEYVVRTFALERRGYSARHEVRQFHFTAWPEHGVPYHA
  TGLLAFIRRVKASTPPDAGPIVIHCSAGTGRTGCYIVLDVMLDMAECEGVVDIYNCVKTLCSRRVNMIQT
  EEQYIFIHDAILEACLCGETTIPVNEFKATYREMIRIDPQSNSSQLREEFQTLNSVTPPLDVEECSIALL
  PRNRDKNRSMDVLPPDRCLPFLISSDGDPNNYINAALTDSYTRSAAFIVTLHPLQSTTPDFWRLVYDYGC
  TSIVMLNQLNQSNSAWPCLQYWPEPGRQQYGLMEVEFVSGTANEDLVSRVFRVQNSSRLQEGHLLVRHFQ
  FLRWSAYRDTPDSRKAFLHLLAEVDKWQAESGDGRTVVHCLNGGGRSGTFCACATVLEMIRCHSLVDVFF
  AAKTLRNYKPNMVETMDQYHFCYDVALEYLEALELR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011214
• ORF Size: 4308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011214.2, NP_035344.2
• RefSeq Size: 5498 bp
• RefSeq ORF: 4311 bp
• Locus ID: 19273
• UniProt ID: B1AUH1
• Cytogenetics: 4 64.5 cM
• Gene Summary: Tyrosine-protein phosphatase which dephosphorylates CTNNB1. Regulates CTNNB1 function both in cell adhesion and signaling. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. May play a role in megakaryocytopoiesis (By similarity).[UniProtKB/Swiss-Prot Function]