Dcun1d3 (NM_173408) Mouse Tagged ORF Clone

CAT#: MG223438

Dcun1d3 (tGFP-tagged) - Mouse DCN1 defective in cullin neddylation 1 domain containing 3 (S. cerevisiae) (Dcun1d3) transcript variant 1, (10ug)

Product Images

Circular map for MG223438

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dcun1d3
• Synonyms: 1700020A13Rik; BC030335
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223438 representing NM_173408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGTGTGTCACCAAGTGCAAGAATCCTTCATCAACCTTGGGCAGCAAGAATGGAGACCGGGACC
  CCAGCAACAAGTCACACAGCAGGCGGGGGGCTAGCCACCGTGAGGAACAGGTGCCACCTTGTGGCAAACC
  AGCTGGGGATATTCTTGTCAATGGGACCAAGAAAGCAGAGGCTGCCACTGAGGCCTGCCAGCTGCCAACT
  TCTTCTGGAGATGCTGGGAGGGAGTCCAAGACCAATGCTGAGGAGTCTTCTTTGCAGAGGTTGGAAGAAC
  TATTCAGGCGCTACAAGGATGAGCGGGAGGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTGCAATGA
  CCTATGTGTTGATCCTACAGAATTTCGAGTGCTGCTCTTGGCCTGGAAGTTCCAGGCTGCTACCATGTGC
  AAATTCACCAGGAAGGAGTTTTTTGATGGCTGTAAAGCAATAAGTGCAGACAGCATTGATGGGATCTGTG
  CACGGTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAAGATAAATTCAAGGATCTCTACCGGTTTACATT
  TCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGGTCACTGCATCGTGAAATAGCCATTGCCCTATGGAAA
  CTAGTATTTACACAGAACAATCCTCCAGTATTGGACCAGTGGCTAAATTTCCTAACAGAGAACCCTTCGG
  GAATCAAGGGCATCTCCCGGGACACTTGGAACATGTTTCTTAACTTCACTCAGGTGATTGGCCCCGACCT
  CAGCAACTACAGTGAAGATGAGGCCTGGCCGAGTCTCTTTGATACCTTTGTGGAGTGGGAAATGGAGAGA
  AGGAAAAGAGAAGTGGAAGGGAGAGGTACACTCAGCTCAGGGCAAGAGGGCTTGTGTCCAGAAGAGCAGA
  CT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223438 representing NM_173408
  Red=Cloning site Green=Tags(s)
  
  MGQCVTKCKNPSSTLGSKNGDRDPSNKSHSRRGASHREEQVPPCGKPAGDILVNGTKKAEAATEACQLPT
  SSGDAGRESKTNAEESSLQRLEELFRRYKDEREDAILEEGMERFCNDLCVDPTEFRVLLLAWKFQAATMC
  KFTRKEFFDGCKAISADSIDGICARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAIALWK
  LVFTQNNPPVLDQWLNFLTENPSGIKGISRDTWNMFLNFTQVIGPDLSNYSEDEAWPSLFDTFVEWEMER
  RKREVEGRGTLSSGQEGLCPEEQT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173408
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173408.3, NP_775584.1
• RefSeq Size: 5637 bp
• RefSeq ORF: 915 bp
• Locus ID: 233805
• UniProt ID: Q8K0V2
• Cytogenetics: 7 F2
• Gene Summary: Antagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane.[UniProtKB/Swiss-Prot Function]