Ablim2 (NM_177678) Mouse Tagged ORF Clone

CAT#: MG223383

Ablim2 (tGFP-tagged) - Mouse actin-binding LIM protein 2 (Ablim2) transcript variant 3, (10ug)

Product Images

Circular map for MG223383

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ablim2
• Synonyms: AI606905; C230091L11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223383 representing NM_177678
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCAGTGTCGCAGCCCCAGGCTGCTCATGCCCCACTGGAGAAGCCGGCCAGCACTGCGATCCTGT
  GTAATACCTGTGGGAATGTGTGCAAGGGAGAGGTGCTGCGTGTGCAGAACAAGTACTTCCACATCAGGTG
  CTTTGTGTGCAAAGCGTGTGGCTGTGACCTGGCTGAGGGTGGCTTCTTCGTGAGGCAGGGTGAGCACATT
  TGCACACGTGACTACCAGCGGCTCTATGGCACTCGCTGCTTCAGCTGTGACCGCTTCATTGAAGGCGAAG
  TGGTGTCGGCGCTTGGGAAGACCTACCACCCTGACTGCTTCGTGTGTGCTGTCTGCAGGCTGCCCTTTCC
  TCCTGGGGACCGTGTGACCTTCAACGGGAAGGAGTGTATGTGCCAGAAGTGTTCCCCACCCACATTGCTG
  GGCAACAGTGCTCACGTGGCCCAGGGCCTCCGGAGTTGTGGGGGCTGTGGCTTAGAAATCAAGAATGGCC
  AGGCCCTGGTGGCTTTGGACAAGCACTGGCACCTGGGATGCTTCAAGTGCAAGACTTGTGGGAAACTCCT
  CAATGCAGAGTACATCAGCAAGGACGGGCTGCCCTACTGCGAGGCTGATTATCACAGCAAGTTTGGCATC
  CGCTGCGACGGCTGTGAGAAGTACATCACGGGCCGGGTGCTAGAGGCCGGAGAGAAGCACTACCACCCTT
  CATGTGCGCTATGCGTCAGGTGCGGCCAGATGTTCTCAGAGGGCGAGGAGATGTACCTGCAAGGCTCCTC
  CATCTGGCATCCGGCATGTCGACAGGCAGCCAGGACTGAAGACAAGAGCAAGGAAACCAGGACTTCTTCG
  GAGAGCATTGTCTCCGTGCCTGCCTCCAGCACGTCAGGGTCCCCGAGCCGTGTGATCTACGCAAAGCTTG
  GCGATGAGATCCTGGACTACAGGGACTTGGCTGCTCTTCCCAAAAACAAAGCGATCTACAACATCGACCG
  CCCCGACATGATCTCCTATTCGCCCTACATTAGCCACTCGGCCGTGGGGGACAGGCAGAGCTATGGCGAG
  GGGGATCAGGATGATCGGTCCTACAAGCAGTGCAGGACCTCCAGCCCCAGCTCTGCCGGCTCAGTCAGCC
  TCGGACACTACACCCCAACCTCACGGTCACCCCAGCACTACAGTCGTCCAGGCAGCGAAAGTGGCCGTAG
  CACCCCGAGTCTCTCAGTGCACTCCGACAGCAGACCTCCTTCCTCTACCTACCAGCAGGCTCCGAGACAC
  TTCCACGTCCCAGACACTGGCGTAAAAGATAACATCTATAGGAAACCCCCTATCTACAAACAGCACGCTG
  CCAGGCGATTGGACGTGGAGGACAGCAGCTTTGACCAGGACAGCAGGAAGAAGACAACCTGGCTGCTTCT
  CAAGGGGGATGCAGACACCAGAACTAACTCTCCAGACCTGGACAGTCAGTCGCTGTCCCTCAGCAGCGGG
  ACTGACCAGGAACCTCTCCAAAGAATGGCAGGGGACAGCCTCTACTCACGCTTTCCTTATTCCAAACCTG
  ACACTCTTCCAGGACCCAGGAAGGATGGCCTGGACCTCCGGAATGCCAACCTGGCCCCTTGTGGAGCAGA
  CCCGGATGCCAGCTGGGGCACGCGAGAGTATAAGATATATCCCTATGATTCCCTCATCGTAACAAACCGA
  ATTCGTGTGAAACTGCCCAAAGACGTGGACCGGACACGGCTGGAGAGACACCTATCACCTGAGGAGTTCC
  AAGAAGTGTTTGGGATGAGCATCGAGGAGTTTGACCGCCTGGCCCTGTGGAAGAGGAATGACCTCAAGAA
  GAAAGCCTTGCTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223383 representing NM_177678
  Red=Cloning site Green=Tags(s)
  
  MSAVSQPQAAHAPLEKPASTAILCNTCGNVCKGEVLRVQNKYFHIRCFVCKACGCDLAEGGFFVRQGEHI
  CTRDYQRLYGTRCFSCDRFIEGEVVSALGKTYHPDCFVCAVCRLPFPPGDRVTFNGKECMCQKCSPPTLL
  GNSAHVAQGLRSCGGCGLEIKNGQALVALDKHWHLGCFKCKTCGKLLNAEYISKDGLPYCEADYHSKFGI
  RCDGCEKYITGRVLEAGEKHYHPSCALCVRCGQMFSEGEEMYLQGSSIWHPACRQAARTEDKSKETRTSS
  ESIVSVPASSTSGSPSRVIYAKLGDEILDYRDLAALPKNKAIYNIDRPDMISYSPYISHSAVGDRQSYGE
  GDQDDRSYKQCRTSSPSSAGSVSLGHYTPTSRSPQHYSRPGSESGRSTPSLSVHSDSRPPSSTYQQAPRH
  FHVPDTGVKDNIYRKPPIYKQHAARRLDVEDSSFDQDSRKKTTWLLLKGDADTRTNSPDLDSQSLSLSSG
  TDQEPLQRMAGDSLYSRFPYSKPDTLPGPRKDGLDLRNANLAPCGADPDASWGTREYKIYPYDSLIVTNR
  IRVKLPKDVDRTRLERHLSPEEFQEVFGMSIEEFDRLALWKRNDLKKKALLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177678
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177678.7, NP_808346.3
• RefSeq Size: 3570 bp
• RefSeq ORF: 1839 bp
• Locus ID: 231148
• UniProt ID: Q8BL65
• Cytogenetics: 5 B3
• Gene Summary: May act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.[UniProtKB/Swiss-Prot Function]