Igfals (NM_008340) Mouse Tagged ORF Clone

CAT#: MG223221

Igfals (tGFP-tagged) - Mouse insulin-like growth factor binding protein acid labile subunit (Igfals), (10ug)

Product Images

Circular map for MG223221

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Igfals
• Synonyms: Albs; ALS; mKIAA4111
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223221 representing NM_008340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAGGACAGGAAGCCCAGCCCTGGTGGTGCTTCTGGCTTTCTGGGTGGCACTGGGCCCCTGTT
  ACCTGCAGGGGACAGATCCTGGAGCATCAGCAGATGCCGAGGGCCCCCAGTGCCCTGTCACCTGTACCTG
  CAGCTATGATGACTACACAGATGAGCTCAGCGTCTTTTGCAGTTCAAGGAACCTCACTCAGCTGCCCGAT
  GGCATCCCAGTCAGCACCAGGGCTCTGTGGCTTGACGGAAACAACCTGTCCTCCATCCCCTCAGCGGCCT
  TCCAGAACCTGTCCAGCCTAGACTTCCTCAACCTGCAGGGCAGCTGGCTGAGGAGCCTGGAGCCACAGGC
  ACTGCTGGGCCTGCAGAATCTCTACCATCTGCACCTGGAACGGAACCTGCTCCGGAGCCTAGCTGCAGGC
  TTGTTCAGACACACACCAAGTCTGGCTTCACTCAGTTTGGGCAACAACCTCCTGGGCCGGCTGGAAGAAG
  GGCTGTTCCGGGGCCTCAGTCACCTTTGGGACCTCAACCTGGGTTGGAACAGCCTAGTGGTCCTGCCTGA
  CACGGTGTTCCAGGGCCTGGGCAACCTCCATGAGCTGGTGCTTGCTGGCAACAAACTGACTTACCTGCAG
  CCTGCGCTCTTGTGTGGCTTGGGCGAGCTGCGGGAGCTGGACCTGAGCAGGAACGCTCTCCGCAGCGTCA
  AAGCTAATGTCTTTATACATCTGCCCCGGCTGCAGAAGCTCTACCTGGACCGCAACCTCATCACAGCTGT
  GGCCCCCCGTGCCTTCCTGGGCATGAAGGCACTGCGTTGGCTGGACCTGTCACACAACCGTGTGGCTGGC
  CTCCTGGAGGACACCTTCCCTGGCCTGCTGGGTCTGCATGTCCTGCGCCTGGCACACAACGCCATCACTA
  GCTTGCGGCCGCGTACTTTCAAAGATCTACACTTCCTGGAGGAACTGCAGCTCGGCCACAATCGTATCAG
  GCAGTTAGGTGAGAAGACGTTTGAGGGCCTGGGGCAGCTGGAGGTACTGACGCTCAATGACAATCAGATC
  CATGAGGTCAAGGTGGGCGCCTTCTTTGGCCTCTTCAACGTGGCTGTTATGAATCTCTCCGGCAACTGTC
  TGAGGAGCCTCCCCGAGCATGTGTTCCAAGGGCTGGGCAGGCTGCACAGCTTGCACCTGGAGCACAGCTG
  CCTGGGCCGCATCCGCCTGCACACTTTCGCCGGCCTCTCAGGGCTGCGCAGGCTCTTCCTCCGGGACAAC
  AGCATCTCCAGCATCGAAGAACAGAGCCTGGCAGGGCTCTCAGAGCTCCTGGAACTCGATCTTACCGCCA
  ACCAGCTCACACATCTGCCCCGCCAGCTTTTCCAGGGCCTTGGCCAGCTGGAATATCTGCTTCTGTCCAA
  CAACCAACTGACAATGCTCTCTGAGGATGTCCTGGGCCCTCTGCAGCGGGCCTTCTGGCTGGACCTCTCA
  CACAACCGCCTCGAGACCCCGGCTGAAGGCCTTTTCTCATCTCTGGGGCGGCTTCGCTACCTCAACCTCA
  GGAATAACTCCTTGCAGACTTTTGTGCCACAGCCTGGCCTGGAGCGCCTGTGGCTCGATGCCAACCCCTG
  GGACTGCAGTTGTCCCCTCAAGGCGCTTCGTGACTTTGCCCTACAGAACCCTGGTGTTGTCCCCCGCTTT
  GTTCAGACTGTCTGTGAGGGAGATGACTGCCAGCCGGTGTACACTTACAACAACATCACTTGTGCTGGCC
  CCGCCAACGTCTCAGGCCTCGACCTTCGAGACATCAGTGAAACACTCTTTGTGCACTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223221 representing NM_008340
  Red=Cloning site Green=Tags(s)
  
  MALRTGSPALVVLLAFWVALGPCYLQGTDPGASADAEGPQCPVTCTCSYDDYTDELSVFCSSRNLTQLPD
  GIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAG
  LFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQ
  PALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAG
  LLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQI
  HEVKVGAFFGLFNVAVMNLSGNCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDN
  SISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGPLQRAFWLDLS
  HNRLETPAEGLFSSLGRLRYLNLRNNSLQTFVPQPGLERLWLDANPWDCSCPLKALRDFALQNPGVVPRF
  VQTVCEGDDCQPVYTYNNITCAGPANVSGLDLRDISETLFVHC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008340
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008340.1
• RefSeq Size: 2111 bp
• RefSeq ORF: 1812 bp
• Locus ID: 16005
• UniProt ID: P70389
• Cytogenetics: 17 12.53 cM
• Gene Summary: May have an important role in regulating the access of circulating IGFs to the tissues.[UniProtKB/Swiss-Prot Function]