Cnbd2 (NM_027585) Mouse Tagged ORF Clone

CAT#: MG223147

Cnbd2 (tGFP-tagged) - Mouse RIKEN cDNA 4921517L17 gene (4921517L17Rik), (10ug)

Product Images

Circular map for MG223147

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cnbd2
• Synonyms: 4921517L17Rik; 5430421B09Rik; Cris
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223147 representing NM_027585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGATCCGCGAATCCGGAAGCCGCCTCCTCTACCAGTCACGTGAAGTTTGATCTGGGGAAGTCCG
  TAGACATCAGCTCTACTGACACTAAGGATGGCGGCACTGCAAGGTCCCCATTGGAGCCGGCGGACAAGTC
  GGACACGACAGAGTCGAAATCTGAGTCGGGATCGGATTCCAGATCAGAGGAAGACAAGGAGAGTCCGGCC
  TCGATTAAGGAGATAAAGGCGGAGACACCCCAACCGAAGGACCGTCCTGGCGTGCAGATTAAGCTCTCAT
  GGTCACAGAAGATAAAGAGCTGGACAGCAAAAAAGAAAAGAAAACTGTACCAGCTCGTCATAGACATCAT
  CATGATGAACCGAGTGTGTAAGATGTTCCGTCAAGGCCTCAGGGGATTTCGGGAATATCAGATCATTGAG
  CCTGTTCACAAGAAGCATCCTGACTTCTCCTTCTGGGATAAAAAGAAGCAAGGCCGTATCTCGTTTGTTA
  CCGAGGACTTTGCTGCACAGGAGGGTCACTTCCCTCCGAGGGCCATTTCTATCACACAGAAGAAGCCATC
  CTGGAGGACCCACCAGGAGATCCAAGACCTCTGCAATATTCTTCAGGCTCTAGATTGCTACCGCAGCTAC
  ACAGAGTCTCTACAGCTGCTCCTGGCCAAGGTCATACGCTTTGAAAGGTTCGGTCGCCGGCGCGTCATCG
  TCAAGAAGGGGCAGATGGGCAATAGCTTTTATTTCATCTACCTGGGCACAGTGGCCATAACTGAGGACGA
  GGATGGGAGCAGTGCTTTCCTGGACCCTCACCCAACACTGCTGCACAGGGGTGGCTCCTTCGGGGAAATG
  GGCCTTCTAAGCACCACAGTAAGGAGTGCCACGGTGGTCTGCATGGAGGAGACAGAGTTCCTGGTGGTCG
  ACAGAGAGGATTTCGTTGCAAATAAGCTGGGTGACGAAGTTCAGAAGGAAACTCAGTATCGGTACAATTT
  TTTTAGGAACTTGGACATATTCCAGTCTTGGTCTGAAGAGAAGCTCTGGAAGCTGGTGGCCTTGGGGAGG
  ATAGAGAGGTTCTCATACGGACAGATGGTGTCTAAAGACTTTATGAATTCGGCATTCATCACATTTATCT
  GCCAGGGCAACTGTGAAATCCTGCGATTGGTAGCCCTGGGCGACTGCAGTGCCTATTACAAGTGGGTCTG
  GCAGCAACTGGAGCTGCTGGATCACAAACCTCTGAGAATTCATGACAATGAAATATCTCCAAAGGAGAGA
  TTTAAGGAACTCCAGATCAAATCCTATCCTCTGCAGGATTTCACGTATTTGAAACTTCTGCGTCTCCAGA
  AAGCCAGAGAGCAACAGGGAATAGACTTCCACGGCAAGATCAACAAAGTAGAAAACACTCTCCCCAAGTT
  GCTGGGCCCAAAGATCAAATCCAGGTACGGTCATCCAGTCAAATGTTCCATGGTCAACACCAAGTTTGGT
  GAACTCCCCAAGGAGGCCATAGTGGGGGTCTACATGAAGATCCACAAGACAGAGGAAGGAGAGATTGTGG
  GCCTTCACCAGGCCTTTGTCCCGGAAATCCAGCGAGATTGCCGGCCCTTCATCCTTCTGAGCTTGGGATC
  AGAGTTGATACAAGTAAGAAAAGAGAAGTTTTATGACATGGTTGACGAGGAGACAAGAGCAAAGATAATA
  AAGATGGATGTGGACTATCCCAGCGATGAAGACCTGTGCCAGAGTTTCCTGAAGGAAAACGACTATATTG
  TCTTTCGGAAGGATTTGCTGCAGTTACTGGTGGAGCCCCTCAATAAGTCACCATTCATCCCAGTCCAGAC
  CAAGAAGAAAGAGATCTACAACCACAAGTCTTTGTTCCTGGATCTGTGCAGCCTTGAAAAGAAGGTGAAG
  CAGCACTATCCCATTTTTCTGGCGCCCCAGAAATACCTGCCCCCCTTGAGGGTTGTCCAAGCCATCTCAG
  CACCCCGGCACAAAATCCAAGAACTCCTGCCTCAATATAAGAATGCAGGGGTCCTTATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223147 representing NM_027585
  Red=Cloning site Green=Tags(s)
  
  MDRSANPEAASSTSHVKFDLGKSVDISSTDTKDGGTARSPLEPADKSDTTESKSESGSDSRSEEDKESPA
  SIKEIKAETPQPKDRPGVQIKLSWSQKIKSWTAKKKRKLYQLVIDIIMMNRVCKMFRQGLRGFREYQIIE
  PVHKKHPDFSFWDKKKQGRISFVTEDFAAQEGHFPPRAISITQKKPSWRTHQEIQDLCNILQALDCYRSY
  TESLQLLLAKVIRFERFGRRRVIVKKGQMGNSFYFIYLGTVAITEDEDGSSAFLDPHPTLLHRGGSFGEM
  GLLSTTVRSATVVCMEETEFLVVDREDFVANKLGDEVQKETQYRYNFFRNLDIFQSWSEEKLWKLVALGR
  IERFSYGQMVSKDFMNSAFITFICQGNCEILRLVALGDCSAYYKWVWQQLELLDHKPLRIHDNEISPKER
  FKELQIKSYPLQDFTYLKLLRLQKAREQQGIDFHGKINKVENTLPKLLGPKIKSRYGHPVKCSMVNTKFG
  ELPKEAIVGVYMKIHKTEEGEIVGLHQAFVPEIQRDCRPFILLSLGSELIQVRKEKFYDMVDEETRAKII
  KMDVDYPSDEDLCQSFLKENDYIVFRKDLLQLLVEPLNKSPFIPVQTKKKEIYNHKSLFLDLCSLEKKVK
  QHYPIFLAPQKYLPPLRVVQAISAPRHKIQELLPQYKNAGVLI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027585
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027585.2, NP_081861.2
• RefSeq Size: 2106 bp
• RefSeq ORF: 2022 bp
• Locus ID: 70873
• UniProt ID: Q9D5U8
• Cytogenetics: 2
• Gene Summary: Essential for male fertility. Plays an important role in spermatogenesis and regulates sperm motility by controlling the development of the flagellar bending of sperm (PubMed:24339785).[UniProtKB/Swiss-Prot Function]