Lrrc17 (NM_028977) Mouse Tagged ORF Clone

CAT#: MG223031

Lrrc17 (tGFP-tagged) - Mouse leucine rich repeat containing 17 (Lrrc17), (10ug)

Product Images

Circular map for MG223031

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lrrc17
• Synonyms: 37kDa; 4833425M04Rik; 6130400C22Rik; P37nb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223031 representing NM_028977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCATAGTGGCCATCTTGCTTCTGTTCTGCCTTTGCAGAGCTGCTGAGCCTCGCAAGTCCAGCCCTG
  GTGTTTTGAGAAGCCAAGGCAATCCCAGCCGCAGCCACGGCCGTGGTGGCAGGAGGGGCTCCAGCCCAGT
  CAAACGCTATGCACCAGGCCTGCCTTGTGATGTGTACACTTATCTCCATGAAAAATACCTAGACTGCCAA
  GAAAGGAAACTAGTCTATGTGTTGCCTGACTGGCCTCAAGATTTGCTGCACATGCTGCTAGCAAGAAACA
  AAATCCGCGTGTTAAAGAACAACATGTTTGCTAAGTTTAAAAGGCTGAAGAGCCTGGACCTGCAGCAGAA
  CGAGATCTCCAAAATTGAGAGCGAGGCGTTCTTTGGTTTAAATAAACTTACCACCCTCTTGTTGCAGCAC
  AACCAGATCAAGGTGCTGACAGAGGAGGCGTTCATTTACACGCCGCTCCTGAGCTACCTGCGGCTCTATG
  ACAACCCCTGGCACTGCACTTGTGAGCTAGAGACGCTCATTTCCATGCTGCAGATTCCACGGAACCGGAA
  TCTGGGGAACTACGCCAAGTGCGGAAGCCCACCAGCACTGAGAAATAAAAAGTTGCTGCAGCTAAAACCT
  CAAGAGCTGTGTGATGAAGAGGAGAAGGAGCAACTGGACCCCAAACCCCAAGTGTCGGGGATACCCGCAG
  TCATCAGGCCAGAAGCAGACTCCACTCTGTGCCACAATTACGTGTTTCCCATACAAACCCTAGACTGCAA
  GAGGAAAGAGTTGAAGAAAGTCCCAAGCAACATCCCTCCAGACATAGTGAAGCTTGACCTGTCATCCAAC
  AAAATCAGACAGCTCCGACCCAAGGAGTTTGAAGATGTTCACGAACTAAAGAAGTTAAACCTAAGCAGCA
  ATGGCATTGAGTTTATAGATCCTGCTGCCTTTTTAGGGCTCATACACCTAGAAGAGCTCGACTTATCAAA
  CAACAGCCTGCAGAACTTTGACTATGGCGTGTTAGAGGATTTGTACTTTCTGAAACTCCTGTGGCTCAGA
  GATAACCCTTGGAGGTGTGACTACAGCATCCACTACCTCTACTACTGGTTAAAGCACCACTACAACGTCC
  ACTATAACGGTCTGGAATGCAAAACGCCGGAAGAATACAAAGGGTGGTCTGTGGGGAAATACGTACGGAG
  TTACTATGAAGAATGCCCCAAGGACAAGCTGCCAGCCTACCCTGAGACGTTTGACCAGGACACAGAAGAT
  GATGAATGGCAGAAAATACACAGAGATCACCCAGCAAAGAAGCACAGAGTGAGAATCACCATTGTGGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223031 representing NM_028977
  Red=Cloning site Green=Tags(s)
  
  MRIVAILLLFCLCRAAEPRKSSPGVLRSQGNPSRSHGRGGRRGSSPVKRYAPGLPCDVYTYLHEKYLDCQ
  ERKLVYVLPDWPQDLLHMLLARNKIRVLKNNMFAKFKRLKSLDLQQNEISKIESEAFFGLNKLTTLLLQH
  NQIKVLTEEAFIYTPLLSYLRLYDNPWHCTCELETLISMLQIPRNRNLGNYAKCGSPPALRNKKLLQLKP
  QELCDEEEKEQLDPKPQVSGIPAVIRPEADSTLCHNYVFPIQTLDCKRKELKKVPSNIPPDIVKLDLSSN
  KIRQLRPKEFEDVHELKKLNLSSNGIEFIDPAAFLGLIHLEELDLSNNSLQNFDYGVLEDLYFLKLLWLR
  DNPWRCDYSIHYLYYWLKHHYNVHYNGLECKTPEEYKGWSVGKYVRSYYEECPKDKLPAYPETFDQDTED
  DEWQKIHRDHPAKKHRVRITIVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028977
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028977.1, NP_083253.1
• RefSeq Size: 2204 bp
• RefSeq ORF: 1332 bp
• Locus ID: 74511
• UniProt ID: Q9CXD9
• Cytogenetics: 5 A3
• Gene Summary: Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors.[UniProtKB/Swiss-Prot Function]