Zcchc8 (NM_027494) Mouse Tagged ORF Clone

CAT#: MG222992

Zcchc8 (tGFP-tagged) - Mouse zinc finger CCHC domain containing 8 (Zcchc8), (10ug)

Product Images

Circular map for MG222992

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zcchc8
• Synonyms: 5730565F05Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222992 representing NM_027494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGGTGTGGATTTTGGCGACCTAGAGCTCTTCGAGGCGTTTGACCCACCAGAGGAGTCGACGC
  CGAAGCCCGTTCACACCCGGTTCAAGGACGACGAGGAGGAAGAGGACGACGACGACGACGAGAACGGGGT
  GGGCGATGCGGAGCTGCAGGAGCAGCTCCGGCGCTGCGAGGCCACCATCGAGCAGCTCCGCGCCGAGAAT
  CAAGAACTTAAAAGAAAGTTGAACATTCTGACAAGACCCAGTGGTATACTGGTGAGCAATACTAAGATAG
  ATGGACCGTTACTACAAATTCTATTTATGAACAATGCTATCTCAAAGCAATACCACCAAGAAATAGAGGA
  GTTTGTATCAAACTTAGTAAAACGATTTGAGGAACAGCAAAAAAATGACGTGGAAAAGACTTCCTTCAGC
  CTTTTACCCCAGCCGTCCAGTGTTATGTTGGAAGAGGACCATAAAGTGGAAGAATCATGTGCCGTTAAAA
  ACAACAAGGAAGCTTTTAGTGTTGTAGGAAGTGTCCTGTATTTTACTAATTTTTGCCTTGATAAATTGGG
  GCAACCGCTACTAAATGAAAACCCTCAGCTTACGGAAGGATGGGAAATACCCAAGTACCAGCAAGTCTTC
  AGCCACATTGTTCCTCTAGAAGGGCAAGAGATGCAAGTGAAGGCAAAAAGGCCAAAGCCTCACTGTTTCA
  ACTGTGGTTCTGAAGAGCATCAGATGAAGGAATGCCCAATGCCTCGGAATGCTGCTCGGATCAGTGAGAA
  GAGGAAAGAGTACATGGATGCCTGTGGCGAGGCCAGCGGCCAGAGCTTCCAGCAGCGCTATCATGCCGAA
  GAAGTAGAAGAGAGATTTGGAAGATTCAAGCCAGGAGTTATTAGTGAGGAACTTCAAGATGCACTGGGTG
  TGACAGACAAGAGTCTTCCCCCTTTCATCTACCGGATGCGCCAGCTGGGCTACCCACCGGGCTGGCTCAA
  GGAGGCTGAACTGGAGAATTCTGGACTTGCACTCTATGATGGAAACGATGACGCTGATGGGGAAACAGAA
  ACTGGAGAAATACAGAATAAAAATGTCACCTACGATCTCTCAAAATTGGTAAACTATCCAGGTTTTAATA
  TATCTACTCCCAGAGGTATTCCAGATGAATGGAGAATGTTCGGTTCCATACCAATGCAGGCGTGTCAACA
  GAAGGATGTGTTTGCCAGTTACCTTAATTCTAACATCCAGTCGCCGAGTATGAGATCTAGCGGCAAGCGG
  TCTTCCTCGCAGTCCAGCCCTAACAGTCCAAAGAAGCAGCGAAAGGAGGGCAGTGCGGCGGCCTCCCCTG
  CCGACATGGAGCTAGACTCAGATGTAGAGATTCCACCTGGTTCTCAGAGCAGCAAAGCATTTCAGTTCCA
  ACCACCGTTGCCTCCCGGAACACCGCCTCCACTCCCACAGGGAACTCCCCCGCCTCTCTTCACCCCTCCA
  CTCCCTAAGGGAACCCCACCACTGACTCCCAGTGACTCACCCCAGGCCCGACCCGCAGCCTCAGCCATGG
  ATGAGGATGCCCTGACGCTGGAGGAGCTGGAAGAGCAGCAGAGGCAGATCTGGGCGGCTCTCCAGCAGGC
  CGAGGGAGGGAATGGCGACTCAGATGTTCCTGGAGACACACCTTTAACTGGGAACTCAGTGGCCTCCTCC
  CCGTGTCCAAACGAGTTTGACCTCCCTGTCCCCGAAGGAAAGGCCCTGGAAAAGCCAGTGCTGGCTGAGC
  CCCAGGAACCAGCTGCTTCTGTAGACACAGCTGGACCCGAGCCATCCTGCAGCCCAGCAGCAGGGGCAGC
  AGTGCTCTCTCAGAGAGAAGAGGAAGCTGCTGCTGAGGGGGGCCCGGGAGATGCTCTTCTCGACAATGGC
  GGTGTGTTGAACATGAACATGAGCAATGGGAGCAACCAGCAGCCCATTCACCCGGACAGCCGGCCTCCGA
  TGGCCCCAAAAACCCATAGCCCAGTACCTGACATGAGCAAGTTTGCCACTGGAATAACACCCTTTGAATT
  TGAGAACATGGCTGAATCCACTGGAATGTACCTCAGGATAAGAAACCTGTTGAAGAATTCACCCCGAAAT
  CAGCAGAAAAACAAAAAGACTTGTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222992 representing NM_027494
  Red=Cloning site Green=Tags(s)
  
  MAAGVDFGDLELFEAFDPPEESTPKPVHTRFKDDEEEEDDDDDENGVGDAELQEQLRRCEATIEQLRAEN
  QELKRKLNILTRPSGILVSNTKIDGPLLQILFMNNAISKQYHQEIEEFVSNLVKRFEEQQKNDVEKTSFS
  LLPQPSSVMLEEDHKVEESCAVKNNKEAFSVVGSVLYFTNFCLDKLGQPLLNENPQLTEGWEIPKYQQVF
  SHIVPLEGQEMQVKAKRPKPHCFNCGSEEHQMKECPMPRNAARISEKRKEYMDACGEASGQSFQQRYHAE
  EVEERFGRFKPGVISEELQDALGVTDKSLPPFIYRMRQLGYPPGWLKEAELENSGLALYDGNDDADGETE
  TGEIQNKNVTYDLSKLVNYPGFNISTPRGIPDEWRMFGSIPMQACQQKDVFASYLNSNIQSPSMRSSGKR
  SSSQSSPNSPKKQRKEGSAAASPADMELDSDVEIPPGSQSSKAFQFQPPLPPGTPPPLPQGTPPPLFTPP
  LPKGTPPLTPSDSPQARPAASAMDEDALTLEELEEQQRQIWAALQQAEGGNGDSDVPGDTPLTGNSVASS
  PCPNEFDLPVPEGKALEKPVLAEPQEPAASVDTAGPEPSCSPAAGAAVLSQREEEAAAEGGPGDALLDNG
  GVLNMNMSNGSNQQPIHPDSRPPMAPKTHSPVPDMSKFATGITPFEFENMAESTGMYLRIRNLLKNSPRN
  QQKNKKTCE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027494
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027494.3, NP_081770.3
• RefSeq Size: 4286 bp
• RefSeq ORF: 2130 bp
• Locus ID: 70650
• UniProt ID: Q9CYA6
• Cytogenetics: 5 F
• Gene Summary: Scaffolding subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]