Arpp21 (NM_001177616) Mouse Tagged ORF Clone

CAT#: MG222989

Arpp21 (tGFP-tagged) - Mouse cyclic AMP-regulated phosphoprotein 21 (Arpp21) transcript variant 4, (10ug)

Product Images

Circular map for MG222989

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arpp21
• Synonyms: 0710001E13Rik; AI853636; ARPP-21; D9Bwg1012e; R3hdm3; Tarpp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222989 representing NM_001177616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCAAGGAGGACTGACTCCGACCATACTGGAAGAAGGGCAGACGGAGCCAGAGTCTGCCCCAG
  AAAATGGCATCCTCAAGTCAGAAAGTCTGGATGAGGAGGAGAAGCTGGAACTGCAGCGGCGACTGGCGGC
  TCAGAACCAAGAGAGGAGAAAATCCAAGTCAGGAGCAGGCAAAGGGAAGCTGACCAGAAGTCTTGCTGTC
  TGTGAAGAGTCTTCAGCTAGATCTGGAGGGGAAAGTCACCAGGATCAGGAATCAATTCACTTACAGCTTT
  CCAGTTTCCCCAGCCTGCAAGAGGAGGATAAATCTAGGAAGGATGATTCTGAGAGAGAAAAAGAAAAGGA
  TAAGAACAGAGAGAAACTCTCTGAGAGACCCAAGATCAGAATGTTATCAAAAGATTGCAGCCAAGAATAC
  ACAGATTCTACAGGCATAGACTTACATGGGTTTCTAATTAACACGCTGAAGAACAATTCCAGGGACAGGA
  TGATACTCTTGAAAATGGAGCAGGAAATGATTGATTTCATTGCTGACAGCAATAACCACTATAAAAAGTT
  CCCCCAGATGTCATCCTATCAAAGGATGCTGGTCCATCGGGTGGCAGCGTACTTTGGATTGGACCACAAC
  GTGGATCAAACTGGAAAATCTGTCATCATCAACAAGACCAGCAGCACCAGGATACCAGAGCAAAGGTTTT
  GTGAACATTTAAAAGATGAAAAAAGTGAAGAATCCCAGAAGCGGTTTATCTTGAAGCGAGATAACTCTAG
  TATTGATAAAGAAGACAATCAGTCAGTTTGCTCACAGGAAAGCCTATTTTTGGACAACAGGGCTCACCGA
  GACAGCTCAGGAAGAACTTCCGGGAGCCGGCAGAGCAGCTCAGAGACTGAGCTCAGGTGGCCAGACCACC
  AGCGGGCTTGGAGCAGCACAGATTCGGACAGTTCCAACCGCAATCTGAAGCCCACCATGACCAAGACGGC
  AAGTTTTGGGGGCATCACGGTGCTGACCAGGGGCGACAGCACGTCCAGTACCAGGAGTGCTGGGAAACTG
  TCCAAAACAGGTTCTGAGTCTTCCAGCAGTGCGGGCTCCTCAGGATCACTGTCCCGCACCCACCCACAGA
  GCACGGCCCTGACCTCTAGCGTGGCAGCTGGTTCTCCTGGCTGTATGGCCTATTCAGAGAATGGAATGGG
  AGGCCAGGTTCCTCCCAGCAGCACCAGCTACATCCTCCTCCCACTTGAAAGTGCGACTGGCATCCCACCC
  GGAAGCATCCTTCTTAATCCACACACAGGTCAGCCCTTTGTGAACCCGGATGGAACGCCTGCGATATACA
  ACCCTCCCGGAAGTCAGCAGACACTGCGTGGCACTGTTGGTGGGCAGCCCCAGCAACCTCCACAGCAGCA
  ACCTTCGCCTCAGCCTCAGCAGCAGGTCCAGGCATCACAGCCCCAGATGGCAGGGCCACTGGTCACTCAG
  TCTGTCCAGAGCCTGCAGCCATCTTCCCAGTCTGTGCAATATCCAGCGGTCTCTTTTCCTCCCCAGCATC
  TCCTGCCTATGTCGCCAACGCAGCACTTCCCCTTGAGAGAAGAACTGGCTGCCCAGTTCAGCCAGCTGAG
  CATGAGCCGGCAGTCCTCGGGAGACACTCCTGAACCTCCCTCTGGTACAGTCTACCCAGCCTCTCTCCTG
  CCACAAACGGCTCAGCCACAGAGTTATGTCATCACTTCTGCAGGCCAGCAGCTCTCTACAGGGGGCTTCT
  CGGACTCTGGCCCGCCCATCTCCCAGCAGGTCCTGCAGGCCCCGCCCTCTCCCCAGGGATTTGTACAGCA
  GCCTCCACCTGCACAGATGTCCGTCTATTACTACCCATCTGGTCAGTACCCTACCTCAACCTCACAGCAG
  TACCGACCCCTCGCCTCAGTCCAGTACAGTGCTCAGCGGAGTCAGCAAATACCACAGACCACACAGCAAG
  CAGGTTACCAGCCAGTCTTGTCTGGTCAGCAGGGCTTCCAAGGCATGATGGGAGTACAGCAGTCAGCGCA
  CAGCCAGGGTGTGATGAGCAGTCAGCAAGGAGCCCCGGTGCATGGTGTGATGGTCTCCTACCCAACCATG
  TCTTCTTATCAGGTGCCAATGACCCAAGGCTCTCAAGCAGTGCCCCAGCAGACATACCAACCACCAATCA
  TGCTGCCCAGTCAGGCAGGCCAAGGGTCACTCCCAGCCACCGGAATGCCTGTGTACTGTAACGTCACACC
  GCCAAACCCTCAGAACAACCTAAGGTTGATGGGTCCACACTGCCCCTCCAGCACGGTTCCTGTCATGTCC
  GCTAGTTGCAGAACAAACTGTGGGAACGTGAGCAACGCAGGCTGGCAGGTCAAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222989 representing NM_001177616
  Red=Cloning site Green=Tags(s)
  
  MSEQGGLTPTILEEGQTEPESAPENGILKSESLDEEEKLELQRRLAAQNQERRKSKSGAGKGKLTRSLAV
  CEESSARSGGESHQDQESIHLQLSSFPSLQEEDKSRKDDSEREKEKDKNREKLSERPKIRMLSKDCSQEY
  TDSTGIDLHGFLINTLKNNSRDRMILLKMEQEMIDFIADSNNHYKKFPQMSSYQRMLVHRVAAYFGLDHN
  VDQTGKSVIINKTSSTRIPEQRFCEHLKDEKSEESQKRFILKRDNSSIDKEDNQSVCSQESLFLDNRAHR
  DSSGRTSGSRQSSSETELRWPDHQRAWSSTDSDSSNRNLKPTMTKTASFGGITVLTRGDSTSSTRSAGKL
  SKTGSESSSSAGSSGSLSRTHPQSTALTSSVAAGSPGCMAYSENGMGGQVPPSSTSYILLPLESATGIPP
  GSILLNPHTGQPFVNPDGTPAIYNPPGSQQTLRGTVGGQPQQPPQQQPSPQPQQQVQASQPQMAGPLVTQ
  SVQSLQPSSQSVQYPAVSFPPQHLLPMSPTQHFPLREELAAQFSQLSMSRQSSGDTPEPPSGTVYPASLL
  PQTAQPQSYVITSAGQQLSTGGFSDSGPPISQQVLQAPPSPQGFVQQPPPAQMSVYYYPSGQYPTSTSQQ
  YRPLASVQYSAQRSQQIPQTTQQAGYQPVLSGQQGFQGMMGVQQSAHSQGVMSSQQGAPVHGVMVSYPTM
  SSYQVPMTQGSQAVPQQTYQPPIMLPSQAGQGSLPATGMPVYCNVTPPNPQNNLRLMGPHCPSSTVPVMS
  ASCRTNCGNVSNAGWQVKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177616
• ORF Size: 2367 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177616.1, NP_001171087.1
• RefSeq Size: 3423 bp
• RefSeq ORF: 2370 bp
• Locus ID: 74100
• UniProt ID: Q9DCB4
• Cytogenetics: 9 62.46 cM
• Gene Summary: Isoform 2 may act as a competitive inhibitor of calmodulin-dependent enzymes such as calcineurin in neurons.[UniProtKB/Swiss-Prot Function]