Tcf4 (NM_001083967) Mouse Tagged ORF Clone

CAT#: MG222956

Tcf4 (tGFP-tagged) - Mouse transcription factor 4 (Tcf4) transcript variant 2, (10ug)

Product Images

Circular map for MG222956

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tcf4
• Synonyms: 5730422P05Rik; ASP-I2; bHLHb19; E2-2; E2.2; ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; SEF-2; SEF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222956 representing NM_001083967
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCACCAACAGCGAATGGCTGCCTTAGGGACGGACAAAGAGCTGAGTGATTTACTGGATTTCAGTG
  CGATGTTTTCGCCTCCTGTAAGCAGTGGGAAAAATGGACCAACTTCTTTGGCGAGTGGACATTTCACTGG
  CTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGAACTGGAGGCCATCCAAGCCCGTCCAGGAAC
  TATGGAGATGGGACTCCCTATGACCACATGACTAGCAGGGATCTTGGGTCACATGACAATCTCTCTCCAC
  CTTTTGTCAATTCCAGAATACAAAGTAAAACAGAAAGGGGCTCATACTCATCTTATGGGAGAGAAAACGT
  TCAGGGTTGCCACCAGCAGAGTCTCCTCGGAGGGGACATGGATATGGGCAATCCAGGAACCCTTTCGCCC
  ACCAAACCTGGCTCCCAGTACTATCAGTATTCAAGCAATAATGCCCGCCGGAGGCCTCTTCACAGTAGTG
  CCATGGAGGTACAGACAAAGAAAGTCCGAAAAGTTCCTCCGGGTTTGCCGTCTTCAGTCTACGCTCCTTC
  AGCCAGCACTGCCGACTACAACAGGGACTCGCCAGGCTATCCTTCCTCCAAGCCAGCAGCCAGCACTTTC
  CCTAGCTCCTTCTTCATGCAAGATGGCCATCACAGCAGCGACCCTTGGAGCTCCTCCAGCGGGATGAATC
  AGCCCGGCTACGGAGGGATGCTGGGCAATTCTTCTCATATCCCACAGTCCAGCAGCTACTGTAGCCTGCA
  TCCACATGAACGTTTGAGCTATCCATCCCACTCCTCGGCAGACATCAACTCCAGTCTTCCTCCGATGTCC
  ACGTTCCATCGTAGTGGCACAAACCATTACAGCACCTCTTCCTGCACACCCCCTGCCAACGGAACAGACA
  GTATAATGGCAAACAGAGGAACTGGGGCAGCAGGCAGCTCGCAGACTGGAGACGCTCTAGGGAAAGCCCT
  AGCTTCGATCTATTCTCCTGACCACACGAACAACAGCTTTTCCTCCAATCCTTCAACTCCTGTGGGCTCC
  CCTCCTTCACTCTCAGCAGGCACAGCTGTTTGGTCTAGAAATGGAGGACAGGCCTCGTCATCTCCCAATT
  ATGAAGGACCCTTGCACTCACTGCAAAGCCGAATCGAAGACCGTTTGGAAAGACTGGACGATGCGATTCA
  TGTTCTCCGGAACCACGCAGTGGGCCCGTCCACAGCTGTGCCTGGTGGCCATGGGGACATGCATGGGATC
  ATGGGACCCTCCCACAACGGAGCGATGGGTAGCCTGGGCTCAGGGTACGGAACTAGTCTTCTCTCAGCCA
  ACAGACACTCGCTCATGGTTGGGGCCCACCGTGAAGATGGCGTGGCTCTGAGAGGCAGCCATTCTCTCCT
  GCCAAACCAGGTTCCGGTCCCACAACTTCCGGTCCAGTCTGCAACTTCCCCTGACTTGAACCCACCCCAA
  GACCCTTACAGAGGGATGCCACCAGGCCTCCAGGGCCAGAGCGTGTCTTCTGGTAGCTCTGAGATCAAAT
  CCGACGACGAGGGCGATGAGAACCTGCAAGACACAAAATCTTCTGAGGACAAGAAATTAGATGACGACAA
  GAAGGATATCAAATCAATTACTAGCAATAACGATGATGAGGACCTGACCCCAGAGCAGAAGGCTGAGCGC
  GAGAAGGAACGGAGGATGGCCAATAATGCCCGTGAGCGCCTGAGGGTCCGAGATATCAACGAGGCTTTCA
  AGGAGCTTGGCCGTATGGTGCAGCTCCACCTGAAGAGCGACAAGCCCCAGACCAAGCTCCTGATTCTCCA
  CCAGGCCGTGGCTGTCATCCTCAGCCTGGAGCAGCAAGTTCGAGAAAGGAATCTGAACCCGAAAGCTGCC
  TGTCTGAAAAGAAGGGAGGAAGAGAAGGTGTCCTCAGAGCCTCCCCCACTCTCCTTGGCTGGCCCACACC
  CTGGGATGGGAGACGCAGCGAATCACATGGGACAGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222956 representing NM_001083967
  Red=Cloning site Green=Tags(s)
  
  MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGTGGHPSPSRN
  YGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRENVQGCHQQSLLGGDMDMGNPGTLSP
  TKPGSQYYQYSSNNARRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPAASTF
  PSSFFMQDGHHSSDPWSSSSGMNQPGYGGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMS
  TFHRSGTNHYSTSSCTPPANGTDSIMANRGTGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGS
  PPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAVPGGHGDMHGI
  MGPSHNGAMGSLGSGYGTSLLSANRHSLMVGAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQ
  DPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAER
  EKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAA
  CLKRREEEKVSSEPPPLSLAGPHPGMGDAANHMGQM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083967
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083967.1, NP_001077436.1
• RefSeq Size: 7135 bp
• RefSeq ORF: 2001 bp
• Locus ID: 21413
• UniProt ID: Q60722
• Cytogenetics: 18 E2
• Gene Summary: Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.[UniProtKB/Swiss-Prot Function]