Sox13 (NM_011439) Mouse Tagged ORF Clone

CAT#: MG222824

Sox13 (tGFP-tagged) - Mouse SRY-box containing gene 13 (Sox13), (10ug)

Product Images

Circular map for MG222824

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sox13
• Synonyms: AA407916; AW259412; AW540933; Sox-13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222824 representing NM_011439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATGCAGAGCCCCGTCTCTGTGCAGCTGGCCCCAGACAGCGCTAGCACCATGGTGAACTGCACCA
  TCAAGTCTGAGGAGAAGAAGGAGCCTTGCCACGAGGCTCCCCAAGGTGCAGCCCCTGCAGTGGAGACCCA
  GCCTGGAGACCCGGCCCTGGCCTCTCAGGATGCCACCAACGCTAAAGCTCCTCCTCAGGACTGTGCCTCA
  CCAGAGAGCAGTGGGTCCCCAGAACCCAAGAGACCAGCAGCTTCCGAGGCTGCTTCGGGAAGCCAGGAGC
  GCCTGGACTTCAACCGCAACTTACAGGAGGTTGTGCCAGCCATTGAGAAGCTGCTGTCCAGTGACTGGAA
  GGAGAGGTTTCTGGGAAGAAGCAATGTGGAAGCTAAGGATGTCAAAGGGACCCAAGAGAGCCTGGCTGAG
  AAGGAACTCCAGCTTTTGGTCATGATCCATCAGCTCTCTGCCCTTCGGGACCAGCTCCTTACGGCCCACT
  CTGAGCAGAAGAACATGGCCGCCATGCTGTTTGAGAAGCAGCAGCAACAGATGGAACTGGCCAGGCAGCA
  GCAGGAGCAGATCGCCAAGCAGCAGCAGCAGCTGATTCAACAGCAGCACAAGATCAACCTCCTGCAGCAG
  CAGATCCAGCAGGTTAACATGCCTTACGTCATGATCCCAGCCTTCCCCCCAAGCCACCAGCCTTTGCCTG
  TCACTCCTGACTCCCAGCTGGCTTTACCTATTCAGCCCATTCCCTGCAAGCCAGTGGAATACCCACTGCA
  GCTGCTGCACAGCCCGCCTGCCCCTGTGGTGAAGAGGTCGGGGGTGGCCGCCCACCACCCCCTGCAGGAG
  CCCCCACAACCACTGAACCTCACAGCCAAGCCCAAGGTACCTGAGCTGCCCAACACCTCCAGCTCTCCCA
  GCCTGAAGATGAACAGTTGTGGGCCTCGCCCCGCCAGCCATGGGGCTCCCACAAGGGACCTGCAATCCAG
  CCCCCCCAGCCTGCCTCTGGGCTTCCTCGGTGAAGGGGACGCCGTCACCAAAGCCATCCAGGATGCCCGG
  CAGCTGCTGCACAGCCACAGTGGGGCTTTGGAAAACTCCCCCAATACCCCATTCCGGAAGGACCTCATCA
  GCCTGGACTCCTCCCCGGCCAAGGAGCGGCTGGAGGAGAGCTGTGTGCATCCCCTGGAGGAAGCCATGCT
  GAGCTGTGACATGGATGGCTCCCGTCATTTTTCCGAGTCTCGGAACAGCAGCCACATCAAGAGACCCATG
  AATGCCTTCATGGTGTGGGCCAAGGATGAACGGAGGAAAATTCTCCAGGCCTTCCCAGACATGCATAACT
  CCAGCATCAGCAAGATCCTTGGTTCTCGCTGGAAGTCCATGACCAACCAGGAGAAACAGCCGTACTACGA
  GGAGCAGGCCCGTCTGAGCCGGCAGCACCTGGAGAAATACCCTGACTATAAGTACAAGCCAAGGCCTAAG
  CGTACCTGTGTCGTGGAGGGCCGGCGGCTGCGAGTGGGCGAGTACAAGGCCTTGATGAGGACACGGCGCC
  AGGGTGCTCGCCAAAGCTACACCATCCCCCCGCAGGCTGGCCAGGCACAGGTGAGCTCAGATATCCTGTT
  CCCTCGGGCAGCGGGCTTGCCCCTGGCACGTCCACTGGTGGAGCACTATGACCCCCAGGGCCTGGACCCC
  AACATGCCTGTCATCATCAATACCTGCAGTCTCCGGGAGGAAGGGGAGGGCACAGATGACAGGCACTCGG
  TGGCCGATGGAGAGATGTACCGGTACAGCGAGGACGAGGACTCAGAGGGTGACGAGAAGAGTGATGAGGA
  GCTGGTGGTACTCACAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222824 representing NM_011439
  Red=Cloning site Green=Tags(s)
  
  MSMQSPVSVQLAPDSASTMVNCTIKSEEKKEPCHEAPQGAAPAVETQPGDPALASQDATNAKAPPQDCAS
  PESSGSPEPKRPAASEAASGSQERLDFNRNLQEVVPAIEKLLSSDWKERFLGRSNVEAKDVKGTQESLAE
  KELQLLVMIHQLSALRDQLLTAHSEQKNMAAMLFEKQQQQMELARQQQEQIAKQQQQLIQQQHKINLLQQ
  QIQQVNMPYVMIPAFPPSHQPLPVTPDSQLALPIQPIPCKPVEYPLQLLHSPPAPVVKRSGVAAHHPLQE
  PPQPLNLTAKPKVPELPNTSSSPSLKMNSCGPRPASHGAPTRDLQSSPPSLPLGFLGEGDAVTKAIQDAR
  QLLHSHSGALENSPNTPFRKDLISLDSSPAKERLEESCVHPLEEAMLSCDMDGSRHFSESRNSSHIKRPM
  NAFMVWAKDERRKILQAFPDMHNSSISKILGSRWKSMTNQEKQPYYEEQARLSRQHLEKYPDYKYKPRPK
  RTCVVEGRRLRVGEYKALMRTRRQGARQSYTIPPQAGQAQVSSDILFPRAAGLPLARPLVEHYDPQGLDP
  NMPVIINTCSLREEGEGTDDRHSVADGEMYRYSEDEDSEGDEKSDEELVVLTD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011439
• ORF Size: 1839 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011439.2, NP_035569.2
• RefSeq Size: 3547 bp
• RefSeq ORF: 1842 bp
• Locus ID: 20668
• UniProt ID: Q04891
• Cytogenetics: 1 E4
• Gene Summary: Binds to the sequence 5'-AACAAT-3'.[UniProtKB/Swiss-Prot Function]