Hnf1b (NM_009330) Mouse Tagged ORF Clone

CAT#: MG222599

Hnf1b (tGFP-tagged) - Mouse HNF1 homeobox B (Hnf1b), (10ug)

Product Images

Circular map for MG222599

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnf1b
• Synonyms: HNF-1-beta; HNF-1B; HNF-1Beta; Hnf1beta; LFB3; Tcf-2; Tcf2; vHNF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222599 representing NM_009330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGTGCCCTGCTGAGCTCCGGAGTCACCAAGG
  AAGTGCTGATCCAGGCCTTGGAGGAGTTACTGCCGTCCCCGAATTTCGGGGTGAAGCTGGAGACACTGCC
  CCTGTCCCCCGGGAGCGGGGCGGATCTCGACACCAAGCCGGTTTTCCATACTCTCACCAATGGCCACGCC
  AAGGGCCGCTTGTCTGGGGACGAGGGCTCAGAGGACGGCGACGACTATGACACTCCTCCCATCCTCAAAG
  AGCTCCAGGCGCTCAACACCGAGGAGGCCGCGGAGCAGCGGGCCGAGGTGGACCGGATGCTCAGCGAGGA
  CCCGTGGAGGGCTGCCAAAATGATCAAGGGATACATGCAACAGCACAATATCCCCCAGAGGGAGGTGGTC
  GATGTCACAGGCCTGAACCAATCCCACCTCTCTCAACACCTCAACAAGGGCACCCCCATGAAGACCCAGA
  AGAGAGCTGCCCTGTACACTTGGTACGTCAGAAAGCAACGGGAGATCCTCCGACAGTTCAACCAGACAGT
  CCAGAGCTCTGGAAACATGACAGACAAAAGCAGTCAGGATCAGCTGCTGTTTCTCTTTCCAGAGTTCAGT
  CAACAGAACCAGGGGCCTGGGCAGTCGGAGGACACCTGCTCCGAGCCCACCAACAAGAAGATGCGCCGCA
  ACCGGTTTAAATGGGGGCCCGCATCCCAGCAAATTTTGTACCAGGCCTACGACCGGCAAAAGAATCCCAG
  CAAGGAAGAGAGGGAGGCCTTAGTGGAGGAGTGTAACAGGGCAGAATGTTTGCAACGAGGGGTCTCCCCT
  TCCAAAGCCCACGGCCTAGGCTCCAACTTGGTCACGGAGGTCCGTGTCTACAACTGGTTCGCAAACCGCC
  GGAAGGAAGAGGCGTTCAGGCAGAAGCTGGCCATGGATGCCTATAGCTCCAACCAGACGCACAACCTGAA
  CCCCCTGCTCACCCATGGCTCCCCTCACCATCAGCCAAGCTCCTCTCCACCCAACAAGATGTCAGGAGTG
  CGCTACAACCAGCCGGGAAACAATGAGGTCACTTCCTCTTCGACAATCAGTCACCATGGTAACAGTGCCA
  TGGTGACCAGCCAGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAGTCTCCTCTC
  ACCTGACAGTAAAATGCAGATCACAGTGTCGGGAGGAGGACTGCCTCCCGTCAGCACCTTGACGAATATC
  CACAGCCTCTCCCACCACAATCCCCAGCAATCTCAGAACCTCATCATGACCCCCCTGTCTGGAGTCATGG
  CCATTGCACAGAGCCTCAACACCTCCCAAGCCCAGGGTGTCCCGGTCATTAACAGTGTGGCTAGCAGCCT
  GGCAGCCCTACAGCCCGTCCAGTTCTCTCAACAGCTGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAG
  AGCCCAGGCAGTCACATGGCCCAGCAGCCCTTCATGGCCGCGGTGACTCAGCTACAGAACTCCCACATGT
  ATGCACATAAGCAGGAACCCCCCCAGTATTCCCACACCTCCCGGTTCCCATCTGCAATGGTGGTCACAGA
  TACCAGTAGCATCAACACTCTCACCAGCATGTCTTCCAGTAAACAGTGTCCACTGCAAGCCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222599 representing NM_009330
  Red=Cloning site Green=Tags(s)
  
  MVSKLTSLQQELLSALLSSGVTKEVLIQALEELLPSPNFGVKLETLPLSPGSGADLDTKPVFHTLTNGHA
  KGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVV
  DVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFS
  QQNQGPGQSEDTCSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSP
  SKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSSNQTHNLNPLLTHGSPHHQPSSSPPNKMSGV
  RYNQPGNNEVTSSSTISHHGNSAMVTSQSVLQQVSPASLDPGHSLLSPDSKMQITVSGGGLPPVSTLTNI
  HSLSHHNPQQSQNLIMTPLSGVMAIAQSLNTSQAQGVPVINSVASSLAALQPVQFSQQLHSPHQQPLMQQ
  SPGSHMAQQPFMAAVTQLQNSHMYAHKQEPPQYSHTSRFPSAMVVTDTSSINTLTSMSSSKQCPLQAW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009330
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009330.3, NP_033356.2
• RefSeq Size: 2742 bp
• RefSeq ORF: 1677 bp
• Locus ID: 21410
• UniProt ID: P27889
• Cytogenetics: 11 51.23 cM
• Gene Summary: Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.[UniProtKB/Swiss-Prot Function]