Wnt9a (NM_139298) Mouse Tagged ORF Clone

CAT#: MG222196

Wnt9a (tGFP-tagged) - Mouse wingless-type MMTV integration site 9A (Wnt9a), (10ug)

Product Images

Circular map for MG222196

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt9a
• Synonyms: Wnt14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222196 representing NM_139298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGATGGGTCCCTTCTGGCGCGCTGGCTGGCCGCGGCCTTCGGGCTGACGCTGCTGCTCGCCGCGC
  TGCGCCCTTCGGCCGCCTACTTCGGGCTAACAGGCAGTGAACCCCTGACTATCCTCCCTCTGACCCTGGA
  GACCGAGGCTGCGGCCCAAGCACACTACAAGGCCTGCGACAGGCTGAAGCTGGAGCGCAAGCAGCGCCGC
  ATGTGCCGCAGGGACCCGGGTGTGGCCGAGACACTGGTGGAGGCCGTAAGCATGAGTGCCCTGGAGTGCC
  AGTACCAGTTCCGCTTTGAGCGCTGGAACTGCACCCTGGAGGGCCGCTACCGAGCCAGCCTGCTCAAGCG
  AGGCTTCAAGGAGACTGCTTTCCTCTACGCCATCTCTTCTGCCGGCCTGACGCATGCACTGGCCAAGGCC
  TGCAGTGCAGGCCGCATGGAGCGCTGCACGTGTGATGAGGCACCCGACCTGGAAAACCGCGAGGCCTGGC
  AGTGGGGCGGCTGCGGGGACAACCTCAAGTACAGCAGCAAGTTTGTCAAGGAGTTCCTGGGCCGGCGCTC
  TAGCAAGGATTTGCGAGCCCGAGTGGACTTCCACAACAACCTCGTGGGTGTGAAGGTGATAAAGGCTGGA
  GTGGAAACCACTTGCAAATGCCATGGTGTGTCTGGCTCCTGCACCGTGCGGACCTGCTGGCGGCAGCTAG
  CACCCTTCCACGAGGTGGGCAAGCACCTAAAACACAAATATGAGACCTCGCTCAAGGTGGGCAGCACTAC
  CAATGAAGCCACTGGAGAGGCAGGTGCCATCTCCCCACCGCGGGGCCGGGCTTCTGGGTCAGGAGGTGGC
  GACCCACTGCCCCGAACACCAGAGCTTGTACACCTGGACGACTCTCCCAGCTTCTGCCTGGCTGGCCGCT
  TTTCCCCTGGCACGGCAGGCCGCAGGTGTCACCGGGAGAAGAACTGTGAGAGTATTTGTTGTGGCCGAGG
  CCACAACACACAGAGTCGTGTGGTGACAAGGCCCTGCCAATGCCAGGTCCGCTGGTGCTGCTACGTGGAG
  TGCAGGCAGTGTACACAGAGAGAGGAGGTCTATACCTGCAAGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222196 representing NM_139298
  Red=Cloning site Green=Tags(s)
  
  MLDGSLLARWLAAAFGLTLLLAALRPSAAYFGLTGSEPLTILPLTLETEAAAQAHYKACDRLKLERKQRR
  MCRRDPGVAETLVEAVSMSALECQYQFRFERWNCTLEGRYRASLLKRGFKETAFLYAISSAGLTHALAKA
  CSAGRMERCTCDEAPDLENREAWQWGGCGDNLKYSSKFVKEFLGRRSSKDLRARVDFHNNLVGVKVIKAG
  VETTCKCHGVSGSCTVRTCWRQLAPFHEVGKHLKHKYETSLKVGSTTNEATGEAGAISPPRGRASGSGGG
  DPLPRTPELVHLDDSPSFCLAGRFSPGTAGRRCHREKNCESICCGRGHNTQSRVVTRPCQCQVRWCCYVE
  CRQCTQREEVYTCKG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139298
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139298.2, NP_647459.1
• RefSeq Size: 3318 bp
• RefSeq ORF: 1098 bp
• Locus ID: 216795
• UniProt ID: Q8R5M2
• Cytogenetics: 11 B1.3
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development (PubMed:16818445). Plays a redundant role in maintaining joint integrity (PubMed:16818445).[UniProtKB/Swiss-Prot Function]