Ptprs (NM_011218) Mouse Tagged ORF Clone

CAT#: MG222172

Ptprs (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type S (Ptprs), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptprs
• Synonyms: AL022616; PTP; PTP-NU3; PTPNU-3; PTPsigma; Ptpt9; R-PTP-S; RPTPsigma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222172 representing NM_011218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  
  
  ATGGCGCCCACCTGGAGTCCCAGCGTGGTGTCTGTGGTGGGTCCTGTGGGGCTCTTCCTCGTACTGCTGG
  CCAGAGGATGCTTGGCTGAAGAACCACCCAGGTTTATCAGAGAGCCCAAGGATCAGATTGGAGTGTCGGG
  AGGCGTGGCCTCCTTCGTGTGCCAGGCCACGGGTGATCCTAAGCCACGGGTGACCTGGAACAAGAAGGGC
  AAGAAAGTGAACTCACAGCGCTTCGAGACCATTGACTTTGACGAGAGCTCTGGGGCGGTCCTGAGGATCC
  AGCCACTTCGGACGCCTCGGGATGAGAACGTGTACGAGTGTGTGGCCCAGAACTCGGTGGGCGAAATCAC
  AATTCATGCAAAGCTCACCGTCCTTCGAGAGGACCAGCTGCCTCCTGGCTTCCCCAACATTGACATGGGC
  CCCCAGTTGAAGGTTGTAGAGCGCACACGCACAGCCACCATGCTCTGTGCTGCCAGCGGGAACCCGGACC
  CTGAGATCACCTGGTTTAAGGACTTCCTGCCTGTGGACCCCAGTGCCAGCAACGGGCGGATCAAGCAGCT
  TCGATCAGGTGCCCTGCAGATTGAGAGCAGCGAGGAGACAGACCAGGGCAAGTACGAGTGTGTGGCCACC
  AACAGCGCTGGGGTGCGCTACTCATCACCTGCCAACCTCTACGTGCGAGTCCGCCGTGTGGCCCCACGCT
  TCTCCATCCTGCCCATGAGCCACGAGATCATGCCCGGTGGGAATGTGAATATCACTTGTGTGGCCGTGGG
  CTCACCCATGCCCTACGTGAAATGGATGCAGGGGGCCGAGGACCTGACGCCTGAGGATGACATGCCCGTG
  GGTCGGAATGTTCTAGAACTCACGGATGTCAAGGACTCAGCTAACTACACTTGTGTGGCCATGTCCAGCC
  TGGGTGTGATCGAGGCCGTGGCCCAGATCACTGTAAAATCTCTCCCCAAAGCCCCTGGGACTCCTGTGGT
  GACGGAGAACACTGCCACCAGTATCACTGTCACATGGGACTCGGGCAACCCTGACCCCGTGTCCTACTAC
  GTAATTGAGTATAAGTCCAAAAGCCAGGATGGGCCGTATCAGATCAAAGAAGACATCACCACCACGCGCT
  ACAGCATCGGAGGCCTGAGCCCCAATTCTGAGTATGAGATCTGGGTGTCAGCTGTCAACTCCATTGGCCA
  GGGCCCTCCCAGTGAATCGGTGGTGACCCGCACAGGTGAGCAGGCACCAGCCAGCGCTCCCAGGAATGTT
  CAGGCCCGCATGCTCAGCGCCACCACCATGATCGTGCAGTGGGAGGAGCCTGTGGAGCCCAATGGCCTGA
  TCCGTGGCTACCGTGTCTACTATACCATGGAGCCGGAACACCCAGTGGGCAACTGGCAGAAACACAATGT
  GGACGACAGTCTCCTGACCACTGTGGGCAGCCTGCTGGAAGACGAGACCTACACCGTGCGCGTGCTCGCC
  TTCACGTCGGTGGGCGACGGACCACTGTCAGACCCCATCCAGGTCAAGACCCAGCAGGGAGTTCCTGGCC
  AGCCCATGAACTTGCGGGCTGAGGCCAAGTCAGAGACCAGCATTGGGCTCTCGTGGAGTGCACCACGACA
  GGAGAGTGTCATTAAGTATGAACTGCTCTTCCGGGAGGGCGACCGAGGCCGAGAGGTGGGGCGAACCTTC
  GACCCAACCACAGCCTTTGTGGTGGAGGACCTCAAGCCCAATACGGAGTATGCGTTCCGGCTGGCGGCGC
  GCTCGCCGCAGGGCCTGGGCGCCTTCACCGCGGTCGTGCGCCAGCGCACGCTGCAGGCCAAACCGTCAGC
  CCCCCCTCAAGACGTTAAGTGCACCAGCTTGCGCTCCACGGCCATATTGGTAAGTTGGCGCCCGCCACCG
  CCAGAAACTCACAACGGGGCCCTCGTGGGCTACAGCGTCCGCTACCGACCGCTGGGCTCAGAGGACCCGG
  ACCCCAAGGAGGTGAACAACATACCCCCGACCACCACTCAGATCCTTCTGGAAGCTTTGGAGAAATGGAC
  GGAGTACCGTGTCACCGCCGTGGCTTACACAGAGGTGGGACCAGGGCCCGAGAGCTCGCCCGTGGTCGTC
  CGCACCGATGAGGACGTGCCCAGCGCGCCCCCGCGGAAGGTGGAGGCGGAGGCGCTCAACGCCACAGCCA
  TCCGAGTGCTGTGGCGCTCGCCCACGCCCGGCCGGCAGCACGGGCAGATCCGCGGCTACCAGGTCCACTA
  TGTGCGCATGGAGGGTGCCGAGGCCCGCGGGCCACCGCGCATCAAGGACATCATGCTGGCGGATGCCCAG
  GAAATGGTGATAACGAACCTCCAGCCTGAGACTGCTTACTCTATCACAGTAGCCGCGTATACCATGAAAG
  GCGATGGCGCTCGCAGCAAACCGAAGGTGGTGGTGACCAAGGGAGCAGTGCTGGGCCGCCCCACCCTGTC
  GGTGCAGCAGACCCCCGAGGGCAGCCTGCTGGCGCGCTGGGAGCCCCCCGCGGACGCGGCCGAGGACCCG
  GTGCTTGGCTACCGCCTGCAGTTTGGGCGCGAAGACGCGGCCCCGGCCACGTTGGAGCTGGCTGCGTGGG
  AGCGGCGGTTCGCGGCGCCTGCACACAAGGGCGCCACCTATGTGTTCCGGCTGGCAGCGCGGGGCCGCGC
  GGGGTTGGGCGAGGAGGCCGCGGCAGCGCTGAGCATCCCCGAGGACGCTCCGCGCGGCTTCCCGCAGATC
  TTGGGCGCCGCGGGCAACGTGTCCGCGGGCTCCGTGCTACTGCGCTGGCTGCCACCCGTGCCCGCCGAGC
  GCAACGGCGCCATCATCAAGTACACGGTGTCCGTGCGGGAGGCCGGCGCCCCTGGGCCCGCGACCGAGAC
  GGAGCTGGCGGCGGCCGCCCAGCCGGGGGCCGAGACAGCGCTCACGCTGCGAGGGCTGCGGCCGGAGACG
  GCCTACGAGTTACGCGTGCGCGCACACACGCGTCGCGGCCCGGGCCCCTTCTCACCCCCGCTGCGCTACA
  GGCTCGCGCGGGACCCAGTCTCCCCAAAGAACTTCAAGGTGAAGATGATCATGAAGACTTCAGTGCTGCT
  GAGCTGGGAGTTCCCCGACAACTATAACTCACCCACACCCTACAAGATTCAGTACAATGGGCTCACCCTG
  GATGTGGACGGCCGCACGACCAAGAAGCTGATCACACACCTCAAGCCACACACCTTCTATAATTTCGTGC
  TCACCAACCGTGGCAGCAGCCTGGGGGGCCTGCAGCAGACGGTCACTGCCAGGACCGCCTTTAACATGCT
  CAGTGGCAAGCCTAGCGTCGCCCCGAAGCCCGACAATGACGGTTTCATCGTGGTCTACCTGCCTGATGGC
  CAGAGTCCTGTGACCGTGCAGAACTACTTCATTGTGATGGTCCCACTTCGGAAGTCTCGAGGTGGCCAGT
  TCCCTGTCCTACTAGGTAGTCCAGAGGACATGGATCTGGAGGAGCTCATCCAGGACATCTCCCGGCTGCA
  GAGGCGCAGCCTGCGCCACTCCAGACAGCTGGAGGTGCCTCGGCCCTACATCGCCGCTCGATTCTCCATC
  CTGCCAGCTGTCTTCCATCCTGGGAACCAGAAGCAATATGGTGGCTTTGACAACAGGGGCTTGGAGCCAG
  GCCACCGCTATGTCCTCTTTGTGCTTGCTGTGTTGCAGAAGAATGAGCCTACATTTGCAGCCAGTCCCTT
  CTCAGACCCCTTCCAGCTGGACAACCCGGACCCTCAGCCCATTGTGGACGGCGAGGAGGGCCTCATCTGG
  GTGATTGGGCCTGTGCTGGCCGTGGTCTTCATCATCTGCATCGTGATTGCCATCCTGCTGTACAAGAACA
  AACCTGACAGCAAACGCAAGGACTCAGAGCCCCGCACCAAATGCTTACTGAACAATGCCGACCTTGCCCC
  CCATCACCCCAAGGACCCTGTGGAAATGCGACGCATCAACTTCCAGACACCAGGTATGCTCAGCCACCCA
  CCCATCCCCATCACAGACATGGCGGAGCACATGGAGAGACTCAAAGCCAACGACAGCCTGAAGCTCTCCC
  AGGAGTACGAGTCCATTGACCCCGGGCAGCAATTCACGTGGGAACATTCGAACCTGGAGGCCAACAAGCC
  CAAGAACCGCTATGCCAACGTCATCGCCTATGACCACTCACGAGTCATCCTGCAGCCCCTAGAAGGCATC
  ATGGGTAGTGATTACATCAATGCCAACTATGTGGACGGCTACCGGCGGCAGAATGCATACATTGCCACGC
  AGGGGCCCCTGCCTGAGACCTTTGGGGACTTCTGGCGGATGGTGTGGGAGCAGCGATCGGCCACTGTGGT
  CATGATGACGCGACTGGAGGAGAAATCACGGATCAAATGTGACCAATACTGGCCTAACCGAGGCACCGAG
  ACATACGGCTTCATCCAGGTCACCCTACTAGATACCATGGAGCTGGCTACCTTCTGCGTCAGGACTTTTT
  CTCTACACAAGAATGGCTCTAGCGAGAAGCGTGAGGTGCGACATTTCCAGTTCACGGCATGGCCCGACCA
  CGGGGTACCTGAGTACCCCACGCCCTTCCTGGCATTCCTGCGAAGAGTCAAGACCTGCAACCCGCCTGAT
  GCTGGCCCCATTGTGGTCCACTGCAGCGCGGGTGTGGGGCGCACTGGCTGCTTCATCGTAATTGACGCCA
  TGCTAGAGCGCATCAAGACAGAGAAGACCGTGGATGTGTATGGACATGTGACACTCATGCGGTCGCAGCG
  CAACTACATGGTGCAGACAGAGGATCAGTATGGCTTCATCCACGAGGCGCTGCTGGAGGCTGTGGGCTGC
  GGCAATACCGAGGTCCCTGCTCGCAGCCTCTACACCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCG
  AGCACGTCACGGGCATGGAGCTTGAGTTCAAGAGGCTCGCCAGTTCCAAGGCACACACTTCGCGCTTCAT
  CACCGCCAGCCTGCCTTGCAACAAGTTTAAGAACCGACTGGTGAACATCCTGCCGTACGAGAGCTCGCGT
  GTCTGCCTGCAGCCCATCCGCGGTGTGGAGGGCTCTGACTACATCAATGCCAGCTTTATCGACGGCTATA
  GACAGCAGAAAGCCTACATTGCAACACAGGGGCCACTGGCAGAGACCACAGAGGACTTCTGGCGAGCTCT
  GTGGGAGAACAACTCTACTATTGTCGTAATGCTCACCAAGCTCCGAGAAATGGGCCGGGAAAAGTGCCAC
  CAGTACTGGCCAGCCGAGCGCTCTGCCCGCTACCAGTACTTTGTGGTTGACCCGATGGCAGAGTATAACA
  TGCCACAGTACATTCTGCGTGAGTTTAAGGTCACAGATGCCCGGGATGGCCAGTCCCGGACCGTCCGACA
  GTTCCAGTTCACGGACTGGCCAGAGCAGGGTGCACCCAAGTCAGGGGAAGGCTTCATTGACTTCATCGGC
  CAAGTGCATAAGACCAAGGAGCAGTTTGGCCAGGACGGACCCATCTCAGTGCACTGCAGCGCCGGAGTGG
  GCAGGACCGGAGTGTTCATCACCCTGAGCATCGTGCTTGAGCGGATGCGCTACGAGGGCGTGGTGGACAT
  TTTCCAGACAGTGAAGGTGCTTCGGACCCAGAGGCCTGCCATGGTGCAGACAGAGGACGAGTACCAGTTC
  TGCTTCCAGGCGGCTTTGGAATACCTGGGCAGTTTTGATCATTATGCAACA
  
  
  Protein Sequence

  >MG222172 representing NM_011218
  Red=Cloning site Green=Tags(s)
  
  MAPTWSPSVVSVVGPVGLFLVLLARGCLAEEPPRFIREPKDQIGVSGGVASFVCQATGDPKPRVTWNKKG
  KKVNSQRFETIDFDESSGAVLRIQPLRTPRDENVYECVAQNSVGEITIHAKLTVLREDQLPPGFPNIDMG
  PQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSGALQIESSEETDQGKYECVAT
  NSAGVRYSSPANLYVRVRRVAPRFSILPMSHEIMPGGNVNITCVAVGSPMPYVKWMQGAEDLTPEDDMPV
  GRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSLPKAPGTPVVTENTATSITVTWDSGNPDPVSYY
  VIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNSIGQGPPSESVVTRTGEQAPASAPRNV
  QARMLSATTMIVQWEEPVEPNGLIRGYRVYYTMEPEHPVGNWQKHNVDDSLLTTVGSLLEDETYTVRVLA
  FTSVGDGPLSDPIQVKTQQGVPGQPMNLRAEAKSETSIGLSWSAPRQESVIKYELLFREGDRGREVGRTF
  DPTTAFVVEDLKPNTEYAFRLAARSPQGLGAFTAVVRQRTLQAKPSAPPQDVKCTSLRSTAILVSWRPPP
  PETHNGALVGYSVRYRPLGSEDPDPKEVNNIPPTTTQILLEALEKWTEYRVTAVAYTEVGPGPESSPVVV
  RTDEDVPSAPPRKVEAEALNATAIRVLWRSPTPGRQHGQIRGYQVHYVRMEGAEARGPPRIKDIMLADAQ
  EMVITNLQPETAYSITVAAYTMKGDGARSKPKVVVTKGAVLGRPTLSVQQTPEGSLLARWEPPADAAEDP
  VLGYRLQFGREDAAPATLELAAWERRFAAPAHKGATYVFRLAARGRAGLGEEAAAALSIPEDAPRGFPQI
  LGAAGNVSAGSVLLRWLPPVPAERNGAIIKYTVSVREAGAPGPATETELAAAAQPGAETALTLRGLRPET
  AYELRVRAHTRRGPGPFSPPLRYRLARDPVSPKNFKVKMIMKTSVLLSWEFPDNYNSPTPYKIQYNGLTL
  DVDGRTTKKLITHLKPHTFYNFVLTNRGSSLGGLQQTVTARTAFNMLSGKPSVAPKPDNDGFIVVYLPDG
  QSPVTVQNYFIVMVPLRKSRGGQFPVLLGSPEDMDLEELIQDISRLQRRSLRHSRQLEVPRPYIAARFSI
  LPAVFHPGNQKQYGGFDNRGLEPGHRYVLFVLAVLQKNEPTFAASPFSDPFQLDNPDPQPIVDGEEGLIW
  VIGPVLAVVFIICIVIAILLYKNKPDSKRKDSEPRTKCLLNNADLAPHHPKDPVEMRRINFQTPGMLSHP
  PIPITDMAEHMERLKANDSLKLSQEYESIDPGQQFTWEHSNLEANKPKNRYANVIAYDHSRVILQPLEGI
  MGSDYINANYVDGYRRQNAYIATQGPLPETFGDFWRMVWEQRSATVVMMTRLEEKSRIKCDQYWPNRGTE
  TYGFIQVTLLDTMELATFCVRTFSLHKNGSSEKREVRHFQFTAWPDHGVPEYPTPFLAFLRRVKTCNPPD
  AGPIVVHCSAGVGRTGCFIVIDAMLERIKTEKTVDVYGHVTLMRSQRNYMVQTEDQYGFIHEALLEAVGC
  GNTEVPARSLYTYIQKLAQVEPGEHVTGMELEFKRLASSKAHTSRFITASLPCNKFKNRLVNILPYESSR
  VCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRALWENNSTIVVMLTKLREMGREKCH
  QYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQGAPKSGEGFIDFIG
  QVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKVLRTQRPAMVQTEDEYQF
  CFQAALEYLGSFDHYAT
  
  
• Restriction Sites: Not Cloning Scheme for this gene
• ACCN: NM_011218
• ORF Size: 5721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011218.2, NP_035348.2
• RefSeq Size: 6874 bp
• RefSeq ORF: 5724 bp
• Locus ID: 19280
• UniProt ID: B0V2N1
• Cytogenetics: 17 29.32 cM
• Gene Summary: Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycans (PubMed:19833921, PubMed:21454754, PubMed:22406547). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth (PubMed:21454754). Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection (PubMed:15797710, PubMed:19833921, PubMed:19780196, PubMed:21454754, PubMed:22519304, PubMed:22406547). Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2 (PubMed:21454754). Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb (PubMed:10080191). Functions as tyrosine phosphatase (PubMed:7529177). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (By similarity). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (PubMed:15797710). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120).[UniProtKB/Swiss-Prot Function]