Mgll (NM_001166251) Mouse Tagged ORF Clone

CAT#: MG222167

Mgll (tGFP-tagged) - Mouse monoglyceride lipase (Mgll) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mgll
• Synonyms: AA589436; Ma; Magl; Mgl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222167 representing NM_001166251
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAGCCAAGACTAATGGAAACAGGGCCTAAAGACCCCTGCAGGATGCCTGAGGCAAGTTCACCCA
  GGCGAACTCCACAGAATGTTCCCTACCAGGACCTGCCTCACCTGGTCAATGCAGACGGACAGTACCTCTT
  TTGTAGATACTGGAAGCCCAGTGGCACACCCAAGGCCCTCATCTTTGTGTCCCATGGAGCTGGGGAACAC
  TGTGGCCGTTATGATGAGCTGGCTCATATGTTGAAGGGGCTGGACATGCTGGTATTTGCCCATGACCATG
  TTGGCCATGGGCAGAGTGAGGGAGAGAGGATGGTGGTGTCGGACTTCCAAGTTTTTGTCAGAGATGTGCT
  GCAACACGTGGACACCATCCAGAAGGACTACCCCGACGTCCCCATCTTCCTCCTGGGCCACTCCATGGGC
  GGTGCCATCTCCATCCTAGTGGCTGCAGAGAGGCCAACCTACTTTTCTGGCATGGTCCTGATTTCACCTC
  TGGTCCTTGCCAATCCGGAATCTGCATCGACTTTGAAGGTCCTTGCTGCCAAACTGCTCAATTTTGTCCT
  GCCAAATATGACCTTGGGGCGCATTGACTCCAGCGTGCTGTCTCGGAACAAGTCGGAGGTTGACCTGTAC
  AACTCTGACCCACTCGTCTGCCGAGCAGGGCTGAAGGTGTGCTTTGGCATACAGCTGCTGAATGCCGTCG
  CAAGAGTGGAGCGAGCAATGCCCAGGCTGACACTGCCATTCCTGCTGCTGCAGGGTTCTGCTGACCGGCT
  TTGCGACAGCAAAGGTGCCTACCTGCTCATGGAATCATCCCGGAGTCAGGACAAAACACTCAAGATGTAT
  GAAGGTGCCTATCACGTCCTCCACAGGGAGCTTCCGGAAGTGACCAACTCCGTCCTCCATGAAGTAAACT
  CGTGGGTGTCTCACAGGATAGCAGCAGCAGGAGCTGGGTGTCCACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222167 representing NM_001166251
  Red=Cloning site Green=Tags(s)
  
  MQKPRLMETGPKDPCRMPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEH
  CGRYDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMG
  GAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLY
  NSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMY
  EGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAAGAGCPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166251
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166251.1, NP_001159723.1
• RefSeq Size: 3991 bp
• RefSeq ORF: 960 bp
• Locus ID: 23945
• UniProt ID: O35678
• Cytogenetics: 6 D1
• Gene Summary: This gene encodes a monoglyceride lipase, which catalyzes the hydrolysis of monoglycerides into fatty acids and glycerol. This enzyme is also thought to hydrolyze the endocannabinoid 2-arachidonoylglycerol. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]