Adgrg2 (NM_001079847) Mouse Tagged ORF Clone

CAT#: MG222103

Gpr64 (tGFP-tagged) - Mouse G protein-coupled receptor 64 (Gpr64) transcript variant 3, (10ug)

Product Images

Circular map for MG222103

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adgrg2
• Synonyms: AW212196; B830041D06Rik; Gpr64; Me6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222103 representing NM_001079847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTCTGGTGGGCAGTACAGCCCTGTTGGCAGACCTGAAGAGGTTTTACTGATATACAAGATAT
  TCCTTGTCATCATTTGTTTTCATGTCATTCTCGTTACATCCCTGAAAGAAAACGGTAATTCCAGTTTGTT
  ATCACCATCTGCTGAATCATCTCTTGTCAGTCTCATCCCCTACTCCAATGATGCTGCTTCAGAAGTTTTG
  TCGACTTTAAACAAAACAGGAGTCAAATCCCAGAGAAATATCTGCAATTTGTCATCTCTTTGCAATGACT
  CAGTATTTTTTAGAGGTGAGATAGTGTTTCAACATGATGAAGACCACAATGTTACCCAGAATCAAGATAC
  AGCTAATGGCACCTTCGCTGGAGTCCTGTCTCTAAGTGAACTGAAGCGATCAGAGCTCAACAAAACTCTA
  CAGACCTTAAGTGAGACTTACTTTATAGTGTGTGCTACAGCAGAGGCCCAAAGCACGGTAAACTGTACAT
  TCACAGTAAAACTCAATGAGACCATGAATGTGTGTGCCATGATGGTTACTTTCCAAACTGTACAGATTCG
  GCCAATGGAACAGTGCTGCTGTTCCCCGAGGACTCCCTGCCCTTCCTCACCAGAAGAGTTAGAAAAACTA
  CAGTGTGAACTGCAGGATCCCATTGTCTGTCTTGCTGATCAACCGCATGGCCCACCGTTATCGTCTTCCA
  GCAAGCCTGTTGTACCTCAGGCCACCATTATTTCCCATGTTGCTAGTGACTTCTCTTTGGCTGAACCCCT
  TGATCATGCCCTTATGACCCCAAGCACACCCTCTCTGACACAAGAAAGTAACCTTCCATCTCCTCAGCCT
  ACGATCCCCCTGGCTTCCAGTCCTGCCACTGACTTGCCAGTTCAATCTGTAGTGGTCTCTTCTTTGCCTC
  AAACTGATCTTTCCCACACCCTGTCACCGGTGCAGTCCTCCATTCCCTCTCCTACCACACCAGCCCCATC
  TGTCCCTACAGAACTGGTCACCATCAGCACACCTCCTGGTGAGACAGTTGTCAACACTAGCACTGTTTCT
  GATCTGGAAGCCCAAGTATCCCAGATGGAGAAAGCTTTGTCTTTGGGTAGCTTAGAGCCTAATCTTGCAG
  GCGAAATGGTAAACCGAGTCAGCAAACTCCTTCACTCTCCACCTGCCTTGCTAGCCCCTCTAGCTCAAAG
  GTTGCTAAAAGTGGTAGATGCCATTGGCTTACAGCTGAATTTTTCATCTACAACTATCAGTCTAACTTCA
  CCTTCTTTGGCTCTTGCTGTGATCAGAGTGAATGCCAGTAATTTCAATACCACGACTTTTGCAGCCCAAG
  ACCCAACAAATCTCCAGGTTTCTCTGGAAACCCCACCTCCTGAGAATAGTATTGGTGCCATTACTCTGCC
  CTCATCACTGATGAATAATTTGCCAGCTAATGATGTAGAATTGGCTTCAAGGATTCAGTTCAATTTCTTT
  GAAACACCCGCCCTGTTTCAGGATCCTTCCCTGGAGAACCTCACTCTGATAAGCTATGTCATATCATCAA
  GTGTCACAAACATGACCATCAAGAACTTGACAAGAAACGTGACAGTCGCACTGAAACACATCAACCCAAG
  TCCGGATGACTTAACTGTGAAATGTGTATTCTGGGACTTGGGCAGAAATGGTGGCAAAGGAGGTTGGTCA
  TCTGATGGCTGTTCCGTCAAAGACAAGAGAATGAATGAAACCATCTGTACCTGTAGCCATCTTACAAGTT
  TTGGCATCCTATTGGACCTATCTCGAACATCCTTACCACCAAGTCAAATGATGGCTCTGACATTTATCAC
  GTATATTGGCTGTGGGCTTTCATCAATTTTTCTGTCAGTTACTCTTGTAACCTACATCGCCTTTGAAAAG
  ATCCGGAGGGATTACCCCTCCAAAATCCTCATCCAGCTGTGTGCTGCCCTGCTTCTGCTCAACCTGATCT
  TCCTCCTAGACTCCTGGATTGCGCTGTATAATACCCGAGGTTTCTGCATTGCCGTGGCTGTATTTCTTCA
  CTATTTTCTCTTGGTCTCATTCACATGGATGGGATTAGAAGCATTCCACATGTACCTAGCACTGGTCAAG
  GTGTTTAATACTTACATCCGAAAGTACATCCTTAAATTCTGCATTGTTGGCTGGGGCATACCAGCTGTGG
  TTGTGTCCATCGTCCTGACTATATCCCCAGATAACTATGGGATTGGATCCTATGGAAAATTCCCCAATGG
  CACACCAGATGACTTTTGCTGGATCAACAGCAATGTGGTGTTCTATATCACGGTTGTGGGATATTTCTGT
  GTGATATTTCTACTGAACGTCAGCATGTTCATCGTGGTCTTGGTTCAGCTCTGTCGAATTAAAAAGAAGA
  AGCAGCTGGGAGCCCAGCGCAAAACTAGTATTCAAGACCTCAGGAGTATCGCTGGCCTCACATTTTTACT
  GGGAATTACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAATGTCACCTTCATGTATCTCTTTGCC
  ATCTTTAACACCTTACAAGGGTTTTTCATATTCATTTTTTACTGTGCAGCAAAAGAGAATGTCAGAAAAC
  AGTGGAGGCGGTATCTTTGTTGTGGAAAATTACGGCTGGCTGAAAATTCTGACTGGAGTAAAACTGCTAC
  TAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTATCCAGCTCTTCAAATTCCTTACAGTCAAGCTGT
  AACTCCACTAACTCCACCACACTCCTAGTGAATAGTGATTGCTCAGTGCACGCAAGCGGGAATGGCAATG
  CATCTACCGAGAGGAATGGGGTTTCTTTCAGTGTTCAGAATGGAGACGTGTGCCTTCATGATCTCACTGG
  CAAACAGCATATGTTTAGTGACAAAGAGGACTCCTGCAATGGTAAAAGCCGGATAGCGCTCAGAAGGACT
  TCGAAGCGGGGAAGCTTACACTTTATCGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222103 representing NM_001079847
  Red=Cloning site Green=Tags(s)
  
  MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHVILVTSLKENGNSSLLSPSAESSLVSLIPYSNDAASEVL
  STLNKTGVKSQRNICNLSSLCNDSVFFRGEIVFQHDEDHNVTQNQDTANGTFAGVLSLSELKRSELNKTL
  QTLSETYFIVCATAEAQSTVNCTFTVKLNETMNVCAMMVTFQTVQIRPMEQCCCSPRTPCPSSPEELEKL
  QCELQDPIVCLADQPHGPPLSSSSKPVVPQATIISHVASDFSLAEPLDHALMTPSTPSLTQESNLPSPQP
  TIPLASSPATDLPVQSVVVSSLPQTDLSHTLSPVQSSIPSPTTPAPSVPTELVTISTPPGETVVNTSTVS
  DLEAQVSQMEKALSLGSLEPNLAGEMVNRVSKLLHSPPALLAPLAQRLLKVVDAIGLQLNFSSTTISLTS
  PSLALAVIRVNASNFNTTTFAAQDPTNLQVSLETPPPENSIGAITLPSSLMNNLPANDVELASRIQFNFF
  ETPALFQDPSLENLTLISYVISSSVTNMTIKNLTRNVTVALKHINPSPDDLTVKCVFWDLGRNGGKGGWS
  SDGCSVKDKRMNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSIFLSVTLVTYIAFEK
  IRRDYPSKILIQLCAALLLLNLIFLLDSWIALYNTRGFCIAVAVFLHYFLLVSFTWMGLEAFHMYLALVK
  VFNTYIRKYILKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWINSNVVFYITVVGYFC
  VIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNVTFMYLFA
  IFNTLQGFFIFIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNSLQSSC
  NSTNSTTLLVNSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKEDSCNGKSRIALRRT
  SKRGSLHFIEQM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079847
• ORF Size: 2976 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079847.2, NP_001073316.1
• RefSeq Size: 4676 bp
• RefSeq ORF: 2979 bp
• Locus ID: 237175
• Cytogenetics: X F4
• Gene Summary: Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.[UniProtKB/Swiss-Prot Function]