Myh11 (NM_013607) Mouse Tagged ORF Clone

CAT#: MG222096

Myh11 (tGFP-tagged) - Mouse myosin heavy polypeptide 11 smooth muscle (Myh11) transcript variant 1, (10ug)

Product Images

Circular map for MG222096

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myh11
• Synonyms: AV071570; SM1; SM2; smMHC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222096 representing NM_013607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGAAAGGGCAGCTCAGCGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATGAACAGCC
  CAATGGCTCAGGCCGACTGGGTAGCCAAGAAGCTGGTGTGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC
  AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTCGTGGAGTTGGTGGAAAATGGAAAGAAGGTCACA
  GTTGGCAAAGATGACATCCAAAAAATGAACCCACCCAAGTTCTCTAAGGTGGAGGACATGGCAGAGCTGA
  CGTGCCTCAATGAGGCCTCTGTGCTGCACAACCTGAGGGAGCGATACTTCTCAGGCCTCATCTATACCTA
  CTCTGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGTACCTACCCATCTACTCAGAAAAGATCGTGGAT
  ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCCGACACAGCCTACAGAA
  GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCCGGAAAGACAGAGAA
  CACAAAGAAAGTCATACAGTACTTGGCTGTGGTGGCATCCTCCCACAAGGGCAAGAAAGACAGCAGCATC
  ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCGAAAACCG
  TCAAGAACGACAACTCCTCTCGCTTTGGCAAGTTCATTCGCATCAACTTCGATGTCACTGGTTACATTGT
  AGGTGCCAATATTGAAACATATCTTCTGGAAAAGTCTAGGGCTATTCGACAGGCTAGGGATGAGAGAACA
  TTTCACATCTTCTACTACCTGCTCGCCGGAGCCAAGGAAAAGATGAAAAGTGACCTGCTTTTGGAGAGCT
  TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA
  GGAGACACTGGAAGCCATGTCTATCATGGGCTTCAATGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA
  TCATCTGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG
  ATAACACAGCGGCTCAGAAAGTTTGCCACCTCGTGGGGATTAATGTGACAGATTTCACTAGAGCCATCCT
  GACCCCACGTATCAAAGTTGGACGGGATGTGGTGCAGAAGGCTCAGACCAAAGAACAGGCTGACTTTGCC
  ATCGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTAAACAAGGCCT
  TGGACAAGACCCATCGGCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCTGGGTTTGAAATCTTTGA
  GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACG
  ATGTTCATCCTGGAGCAGGAAGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGG
  ACCTGCAGCCTTGTATTGAGCTGATTGAGCGGCCGAACAACCCTCCCGGTGTGCTGGCCCTGCTGGATGA
  AGAATGCTGGTTCCCCAAAGCTACAGACAAGTCTTTTGTGGAGAAGCTATGCTCAGAGCAGGGAAATCAC
  CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG
  TGGACTACAATGCAAGTGCCTGGCTGACCAAGAACATGGACCCGCTAAATGACAATGTGACATCACTCCT
  CAATGCCTCCTCTGACAAGTTTGTGGCTGACCTGTGGAAGGATGTGGACCGCATTGTGGGGCTGGACCAG
  ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG
  GCCAGCTCTACAAAGAGCAGTTGGGGAAACTGATGACTACACTGCGCAATACCACGCCTAACTTTGTGCG
  CTGCATCATCCCCAACCATGAGAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTGCTGGAACAGCTGCGC
  TGCAACGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT
  TCCGGCAACGCTATGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGATGGAAAGCAAGCCTG
  CATTCTCATGATCAAAGCCCTCGAACTTGACCCTAACCTGTACAGGATTGGGCAGAGCAAAATCTTCTTC
  CGAACGGGGGTCCTGGCCCACCTAGAGGAGGAACGAGACCTGAAAATTACTGATGTCATCATGGCCTTCC
  AGGCAATGTGTCGTGGCTACCTTGCCAGAAAGGCCTTCACCAAGAGGCAGCAACAGCTGACAGCCATGAA
  GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGCGGCTCTTCACCAAA
  GTAAAGCCATTGCTCCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAAAAGA
  TCAAGGAGCGGCAGCAGAAGGCAGAGACTGAGTTGAAGGAGCTGGAGCAGAAGCACACTCAGCTGGCTGA
  GGAGAAGACTCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAGGAGATGCGG
  GTCCGGTTGGCAGCCAAGAAGCAGGAACTGGAGGAGATCCTACATGAGATGGAGGCCCGCCTGGAGGAAG
  AGGAAGACCGGGGCCAGCAACTACAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTAGACCTGGAGGA
  GCAACTGGAGGAGGAAGAGGCCGCCAGACAGAAACTACAGCTAGAGAAGGTCACGGCTGAGGCCAAGATC
  AAGAAACTGGAGGATGACATCTTGGTTATGGATGATCAGAACAGTAAACTTTCAAAAGAACGAAAACTCC
  TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA
  GCTGAAGAGCAAGCATGAGTCTATGATCTCAGAGCTGGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG
  CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCATGAGCAGATCGCTGACT
  TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC
  CAGGCTTGATGAAGAGATCGCCCAGAAAAACAATGCCCTAAAGAAGATTCGCGAGCTAGAGGGCCATATC
  TCAGACCTACAAGAGGACCTAGACTCAGAGCGGGCTGCCAGGAACAAGGCCGAGAAGCAGAAGCGAGACC
  TGGGGGAGGAGCTGGAGGCACTCAAGACGGAGCTGGAGGATACGCTGGACAGCACAGCTACCCAGCAGGA
  GCTCAGAGCCAAGAGGGAACAGGAGGTGACAGTGCTGAAGAAGGCCCTGGATGAGGAGACGCGGTCCCAT
  GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAGC
  AGTTCAAAAGGGCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGC
  TGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAG
  CTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACA
  AGCTACAGAATGAAGTGGAGAGTGTCACTGGCATGCTCAATGAGGCAGAGGGCAAAGCCATCAAACTGGC
  CAAAGATGTGGCTTCCCTTGGATCCCAGCTTCAGGACACCCAAGAGCTGCTCCAAGAAGAAACCCGGCAG
  AAGCTCAATGTGTCTACCAAGCTGCGTCAGTTGGAAGATGAAAGGAACAGCCTGCAGGACCAGCTGGATG
  AGGAGATGGAGGCTAAGCAAAACCTGGAGCGCCATGTCTCAACACTGAACATTCAGCTCTCAGACTCTAA
  GAAGAAGCTGCAGGACTTTGCAAGTACCATCGAGGTCATGGAGGAGGGGAAGAAGAGGTTACAGAAAGAG
  ATGGAGGGCCTCAGCCAGCAGTATGAGGAGAAGGCGGCTGCCTATGACAAACTGGAGAAAACCAAGAACA
  GGCTCCAGCAGGAGCTGGATGACCTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA
  AAAGAAGCAGAAGAAATTTGACCAGTTGTTAGCTGAGGAGAAGAACATCTCCTCCAAGTATGCGGATGAG
  AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAGACAAAGGCTTTGTCTCTAGCCCGGGCCCTGGAGG
  AAGCCCTGGAAGCCAAAGAAGAGCTGGAGAGGACCAACAAGATGCTCAAAGCTGAGATGGAAGACCTGGT
  CAGCTCCAAGGATGATGTAGGCAAGAACGTGCATGAACTGGAGAAGTCCAAGCGTGCCTTGGAGACCCAG
  ATGGAAGAGATGAAAACCCAGCTGGAGGAGTTGGAGGATGAGCTGCAGGCCACTGAGGATGCCAAGCTGC
  GGCTAGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGCGATCTCCAGGCTCGGGATGAACAGAA
  TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCACGAGTATGAGACAGAACTGGAAGATGAACGGAAG
  CAGAGAGCTCTGGCGGCGGCAGCTAAGAAGAAGCTGGAAGGGGACCTAAAAGACCTAGAGCTCCAGGCTG
  ACTCAGCCATCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTGCAGGCTCAGATGAAGGACTT
  CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGGGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG
  AAAGCCAAGAGTCTGGAGGCAGACCTCATGCAGCTCCAAGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA
  AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCCGAGGAGCTGGCTAGCAGCTTGTCAGGAAGGAATACACT
  GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAACTAGAGGAGGAGCTGGAGGAAGAGCAGGGC
  AACATGGAGGCCATGAGTGATAGAGTACGCAAGGCCACACTGCAGGCTGAGCAACTGAGCAATGAGCTGG
  CCACAGAACGCAGCACGGCTCAGAAGAATGAGAGCGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT
  GCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTCAAGTCCACTGTTGCGGCGCTGGAG
  GCCAAGATTGCACAGCTGGAGGAGCAGGTTGAACAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGC
  TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTGCTGCAGGTGGAGGATGAGCGCAAGATGGCAGAGCA
  GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTGAAGAGGCAGCTGGAAGAGGCAGAG
  GAGGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTAGATGAGGCCACAGAGAGCA
  ATGAGGCCATGGGCCGTGAGGTGAACGCCCTCAAGAGCAAACTCAGAGGGCCCCCCCCACAGGAAACTTC
  GCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222096 representing NM_013607
  Red=Cloning site Green=Tags(s)
  
  MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVT
  VGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVD
  MYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSI
  TGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERT
  FHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVV
  SSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFA
  IEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHT
  MFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNH
  PKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQ
  MAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLR
  CNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFF
  RTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTK
  VKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMR
  VRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKI
  KKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSR
  QELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEIAQKNNALKKIRELEGHI
  SDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSH
  EAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQ
  LQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQ
  KLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKE
  MEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADE
  RDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQ
  MEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERK
  QRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEK
  KAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQG
  NMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALE
  AKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAE
  EESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRGPPPQETSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013607
• ORF Size: 5814 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013607.2, NP_038635.2
• RefSeq Size: 6632 bp
• RefSeq ORF: 5817 bp
• Locus ID: 17880
• Cytogenetics: 16 9.71 cM