Slfn10 (NM_181542) Mouse Tagged ORF Clone

CAT#: MG221757

Slfn10 (tGFP-tagged) - Mouse schlafen 10 pseudogene (Slfn10-ps), (10ug)

Product Images

Circular map for MG221757

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slfn10
• Synonyms: mFLJ00257; Slfn10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221757 representing NM_181542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAACGACTAACAAGAGTGAGCATCCTGTGGAGATGGGACAGGATTTGGAAAAGTCTTTGAGAGAGC
  TTATTATGTCCTCTAATATGCAGGGTTTCTTTGAGACCAAGCAACAAGAGGACCAGTTCTACATTTTTGT
  TAAATCTTGGAGCTGCAGCCCTGAAGATGCTTCCACTAAGCCTCGAATTTGCAGCCTGGGCTCTTCCCTG
  TACTGTAGATCTTTAACTTCTAACGTTGCCATGGATTCAAGGGAAGCATTTGACTTTCTGAAGGGCAAGA
  AGAGATGTGTTAAGTGCAGTCCTACTGATGACAGAGCCAATAAAATTCCAGGAGCCATGTATCAGAACAG
  CCTTGAAACAAATCCAGCTTTTGAAATTTTCCAAAGTAAGAAACTTGAATATGGCCAACACTTGCTTTTT
  TCTGAATCCACATTTATACAGTTTAAACAATTCTCTACCAAACATGTCCAAGAATATATAAAAAACAAAA
  TTCCAGAGTACATCTCCGCATTTGCAAACACCCAGGGAGGCTATCTTTTCATTGGAGTGGATGATAAGAG
  TACCATCCTGGGATGCCCAAAAGACAACGTTGACCGTGACTCTTTGAAAACTGTGGCAAATGAAGCAATC
  TCCAAGTTGCCAGTTTTCCATTTTTGTTCATCTAAAGACAAGGACAAGGTGTCTTATGAGACCAGAGTCA
  TAGATGTGTTTCAAGAGGGAAATTTGTATGGTTATCTCTGTGTGATCAAAGTAGAGCCTTTCTGCTGTGC
  AGTGTTCTCAGAGGCTCCCATTTCATGGATGGTAGACAAGGAGAAAGGTGTCTACAGACTGAACACCGAG
  GAATGGGTACGCATGATGGTGGATGTTGGCCCAGAGGCAGCTTCCAAAGATCTATCTAAAGATTTTGAAT
  GTCAGCTGAGTCTATCCAACAGCCCCCCACGCTGCAGACCAGTGTATTCTAAAAAGGGACTGGAATATAA
  AGTCGACCTGCAGCAGCATTTATTTCAAGTGTCACCAGACCATTTAAAGTATACTCCTGAATCTCTCTGG
  AGTGAGCTGTGTTTCGAGCATGAGAGACTAGAGGATTTAGTAAAGCAGCAAATACGTTCTTTCTCCTGTG
  GTTTGCTGATCCTCTCTAGAAGCTGGGCTGTGGACCTGAACCTGGAAGAGAAGCAGGAAGTCATCTGTGA
  TGCTCTGCTGATTGCAGAGAATAGCCCCCCCATCCTCTACACCATCCTTGGGGAGCAGGATGAGCAGGGT
  CAGGACTACTGCACCAGCACTGCCTTTACTCTGAAGCAGAAGCTGGTGAACACTGGTGGCTACACTGGGA
  GAGTGTGTGTCATGACCAAAGTTCTCTGCCTGAGTTCTCAGAACAGTATTGAGACTAATGGGGGCTCAGT
  CTCTCCTATTGATTACCCACACTCCTATAACCTTGCAAACATCCAGGAAATGCAGGACTTGCTGCAGGCC
  CTTGTGATTGTCTTGCTCAACTTCAGATCTTTCTTGAGCGACCAGCTTGGCTGTGAAATTTTGAATCTTC
  TCACAGCCCAACAGTATGAGATACTCTCAAAAAGTCTCCGCAAAACCAGAGAACTATTTGTGCATGGCTT
  GCCGGGCTCAGGGAAGACAATCATAGCCATGAAAATCATGGAAAAGATCAGAAACACATTCCACTGTGAA
  ACAGATAGAATCCTCTACATCTGTGAAAATCAGCCATTGAGGGACTTCATCCAGGCAAAAAATATCTGCC
  AGGCAGTGACCCGGGAAACCTTCATGAAAGATTACTTTGAGACAAACAGGATCCAACATATCATTGTTGA
  TGAAGCCCAGAATTTCTGCACTGAAGATGGGAACTGGTATGAGAAGGCAAAAGGAATCACTCGAAGAATG
  AAATGTTGTCCTGGAATTCTCTGGATCTTTCTGGACTATTTTCAGACCAGTCACTTGAAGAAGAGTGGCC
  TCCCAAATTTCTTATGCCAGTTTCCGACGGAAAAGCTCACACAAATGGTACGCAATGCAGATAAAATAGC
  TGAGTTCCTACAACAAGAGTTGCGAAAAATCAGAGCTAACCCTCCATTCAGCATCCCTCAAGGGTCCCTA
  AGCATGTTCCATGGATTTTACTGGGCTCAGGGTGTATCAGGCACCCATGAGATTACATACTTGACTTTGG
  AAAAGATGGTAAGCTATGTAGCAGATAAGTGTGATGTTTTCTTGAGTAAAGGCTATTCTCCCCAAGATAT
  TGCAGTGCTTTTCAGCACAGACAGGGAAAAGAAAGCCTATGAGCATATGTTCCTGAGAGAAATAAGGAAG
  AGGAAGAGAGCATCTCAGATGAATGACGCGTCTGTCTGTCATTCTAACATGTTTGACAGCATCCGTCGAT
  TCTCAGGACTGGAAAAAAGCATTGTGTTTGGTATCAATCCCAATGCAACTGAGCAGCCCATTTCCCACAA
  CCTATTGCTCTGCCTGGCTTCCAGAGCAACGGAACATCTGTATATCCTGTATCTTTCAACTCCTGAGGGG
  TATAGCTCAACAGTGGCATGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221757 representing NM_181542
  Red=Cloning site Green=Tags(s)
  
  MQTTNKSEHPVEMGQDLEKSLRELIMSSNMQGFFETKQQEDQFYIFVKSWSCSPEDASTKPRICSLGSSL
  YCRSLTSNVAMDSREAFDFLKGKKRCVKCSPTDDRANKIPGAMYQNSLETNPAFEIFQSKKLEYGQHLLF
  SESTFIQFKQFSTKHVQEYIKNKIPEYISAFANTQGGYLFIGVDDKSTILGCPKDNVDRDSLKTVANEAI
  SKLPVFHFCSSKDKDKVSYETRVIDVFQEGNLYGYLCVIKVEPFCCAVFSEAPISWMVDKEKGVYRLNTE
  EWVRMMVDVGPEAASKDLSKDFECQLSLSNSPPRCRPVYSKKGLEYKVDLQQHLFQVSPDHLKYTPESLW
  SELCFEHERLEDLVKQQIRSFSCGLLILSRSWAVDLNLEEKQEVICDALLIAENSPPILYTILGEQDEQG
  QDYCTSTAFTLKQKLVNTGGYTGRVCVMTKVLCLSSQNSIETNGGSVSPIDYPHSYNLANIQEMQDLLQA
  LVIVLLNFRSFLSDQLGCEILNLLTAQQYEILSKSLRKTRELFVHGLPGSGKTIIAMKIMEKIRNTFHCE
  TDRILYICENQPLRDFIQAKNICQAVTRETFMKDYFETNRIQHIIVDEAQNFCTEDGNWYEKAKGITRRM
  KCCPGILWIFLDYFQTSHLKKSGLPNFLCQFPTEKLTQMVRNADKIAEFLQQELRKIRANPPFSIPQGSL
  SMFHGFYWAQGVSGTHEITYLTLEKMVSYVADKCDVFLSKGYSPQDIAVLFSTDREKKAYEHMFLREIRK
  RKRASQMNDASVCHSNMFDSIRRFSGLEKSIVFGINPNATEQPISHNLLLCLASRATEHLYILYLSTPEG
  YSSTVAC
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181542
• ORF Size: 2541 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181542.4, NP_853520.3
• RefSeq Size: 3516 bp
• RefSeq ORF: 2544 bp
• Locus ID: 237887
• Cytogenetics: 11 C