Adamts14 (NM_001081127) Mouse Tagged ORF Clone

CAT#: MG221535

Adamts14 (tGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif 14 (Adamts14), (10ug)

Product Images

Circular map for MG221535

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adamts14
• Synonyms: Adamts-14; TS14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221535 representing NM_001081127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGCTCCGAGCTCTGCTCCACTGCCTGCTGCCCTGGTACTGCGCGCTCTGTGCCGCCGCCGGCA
  GTCAGACCCCAGACCTGCGCCTCTCTGGGAAACTTCACGACTATGTTGTGACGGTGCCTTGCAGCACAGA
  TTTCCAGGGACGCTTCTTGTCCCATGTGGTGTCTGCCCCCGCAGCTCCCTCACCACGGAGCCACCTCCGC
  GTGGCTCGCAGCCCCCTGAGCCTGGAAAGAGAGACCCCGAGGCCTGGCGGTCCGAGGCAGCATTTCCTCT
  ACTTTAATGTGACTGTCTTTGGGAAGCTGCTTCACTTGAGGCTGCAGCCGAACCGGAGGTTGGTGGCCCC
  AGGGGCCCCGGTGGAATGGCAGGAGGACTTTCGGGAACTCTTCCGACAACCCTTGCAGCAGGAGTGTGTG
  TACACTGGAGGTGTCACTGGAATGCCCGGGGCAGCTGTGGCCATCAGCAACTGTGATGGATTGGCCGGCC
  TCATCCGCACAGACAACTCAGACTACTTCATCGAGCCCCTGGAGCGAGGGCAGCAGGAGAAAGAGGCTGG
  TGGGAGGACCCATGTGGTATACCGCCGGGAAGCCGTCCAGAGGGAGTGGAAAGAGCCTCATGGTGACCTT
  CACAACGAAGCCTTTGGCCTTGGCGACCTTCCCAACGTGCTGGATCTGGTGGGGGACCGGCTGGGTGACG
  CAGAGCGGAAGCGGCGACACGCCAAGCCCGGCAGCTACAGCATCGAGGTTCTGCTGGCGGTGGATGACTC
  GGTGGTCCGCTTCCACGGCAGGGAGCATACGCAGAACTACGTGCTGACGCTCATGAATATCGTGGATGAG
  ATTTACCATGACGAATCGCTGGGAGCCCACGTGAACATCGCCCTTGTCCGCCTGATCATGGTGGGCTACC
  GACAGTCCCTGAGCCTGATTGAGCGAGGGAACCCAGCGCGCAGCCTGGAGCAGGTGTGTCGCTGGGCACA
  CTCCCAGCAGCGCCAGGACCCTAGCCACACTGAACATCACGACCACGTCATCTTCCTCACGCGCCAGAAC
  TTCGGCCCCTCGGGGTATGCACCTGTTACTGGGATGTGCCACCCACTAAGAAGCTGTGCCCTCAACCACG
  AGGATGGCTTCTCCTCAGCTTTTGTCGTGGCTCATGAGACAGGCCATGTGCTTGGCATGGAGCATGACGG
  CCAGGGCAATGGCTGCGATGATGAGACCAGTCTGGGCAGCGTCATGGCGCCCCTGGTTCAGGCCGCCTTC
  CACAGGTTCCACTGGTCTCGCTGCAGCAAGCTGGAGCTCAGCCGCTACCTCCCATCCTACGATTGCCTCC
  TTGATGACCCCTTCGAGCGCACCTGGCCCCAGCCCCCAGAACTGCCCGGGATCGACTACTCCATGGATGA
  GCAATGCCGGTTCGACTTTGGCACTGGGTACCACACCTGCTTAGCTTTCAGGACCTTTGAGCCCTGCAAG
  CAGCTATGGTGCAGCCACCCTGACAACCCATACTTCTGCAAGACCAAGAAGGGGCCGCCGCTGGATGGGA
  CAGAGTGTGCACCAGGCAAGTGGTGCTTCAAAGGCCATTGTATCTGGAAGTCACCAGAGCAAACTTATGG
  CCAGGATGGAGGCTGGAGTTCCTGGACCAATTTTGGCTCATGTTCTCGGTCGTGTGGAGGAGGGGTGCGA
  TCCCGAAGCCGGAGCTGCGACAACCCTCCTCCAGCCTATGGAGGCCGCCCGTGCTCAGGGTCCATGTTTG
  AGTACCAGATCTGCAACAGTGAGGACTGTCCTGGGCCCTACGAGGACTTCCGAGCCCAGCAGTGTGCCAA
  GCGAAACTCCTACTATACCCACCAGGATGCCAAGCACAGCTGGCTGCCCTATGAGCCCGACAGTGATGCC
  CAGAAGTGCGAGCTCATTTGCCAGTCTGCCGACACTGGAGATGTGGTATTTATGAACCAAGTAGTCCACG
  ATGGGACACGCTGCAGCTATCGCGATCCTTACAGCGTCTGTGCCCGTGGCGAGTGTGTGCCCTTTGGTTG
  CGACAAGGAGGTGGGATCCATGAAGACGGATGACAAGTGCGGTGTCTGCGGTGGGGACAATTCTCACTGT
  AGGACTGTGAAGGGGACTCTGGGAAAGGGCTCCAAGCAGGCAGCGGCTCTCAAACAGGTGCAGATCCCGG
  CGGGTGCCAGGCACATTCAGATTGAATTGCTGGAGAAGGCTCCCCACCGAATCGCGGTGAAGAACCAGGT
  GACCGGAAGCTTCATCTTCAACCCCAAGGGCAAGGAGGCCTCTAGCAGGACCTTCACTGCGCTGGGCCTA
  GAGTGGGAGCACGAAGCGGAGGACACCAAGGACAGCCTCAGGACCAATGGACCCCTGCCTGAAGCCATCG
  CCATCCTGGTTCTTCCCCCGGCTGAGGGTAAACCCCGAGGTAGCCTGGCCTACAAGTACGTCATCCATGA
  GGACCTGCTGCCCCTCATCGGGAGTAATAATGTGCTCCTGGAAGAGACAGACACCTACGAATGGGCTCTC
  AAGAGCTGGTCTCCCTGCAGCAAGGCCTGTGGAGGAGGAATCCAGTTCACTAAATATGGCTGCCGGCGCC
  GCAGGGACCACCACATGGTGCACCGGCACCTGTGTGACCATAAAAAGAGGCCCAAGCCTATCCGACGGCG
  TTGCAACCAGCACTCGTGTCCCCAGCCCACGTGGGTGACAGAAGAGTGGGGTGCCTGTAGCCGAAGCTGC
  GGGAAGCTGGGGCTGCAGACCCGGGGAGTGCAGTGCCTGCTGCCTCTCTCCAATGGCACCCACAAAGCCA
  TGCCAGCCAAGGCCTGCCTAGGTAACCGGCCAGAGGCCAAGAAGCCATGCCTCCGTGTGCCCTGCCCAGC
  CCAGTGGCGGACAGGAGCCTGGTCCCAGTGCTCTGCTACCTGCGGAGAAGGCATCCAGCAAAGGCAGGTG
  GTATGCAGGAACACTTCCAGTGCCCTCGGGCCATGCGAAGGGGTCAAGCCGGACATGGTGCAGATCTGCA
  GCCTGCCTGCCTGTGGAGGAGATCTCCAGAACTCTACAGTGAAGGCGGAGGTCCAGGACCCTGTGACCAA
  AACAGGATACCGGGAACCCCAATCCAGGCCCCTGACTCCTGAGGACAGGATCTCAACAATGGAGCCCTGT
  GTGCGAGACAGATCCGTCTTCTGCCGAATGGAAGTGTTGGACCGTTACTGCACCATCCCCGGCTACCACC
  GTCTATGCTGTGAGTCTTGCATAAAGAAGACCTCAGGCCCCAATGCCAGCCTGGCCTTACCACCCACCTT
  CTCCACTCCTGGGAGTCTCTTGCCAGCACCCAAGGCCACCCTGGAGGATGTGAAGTCTACAAGGGGGCCA
  ACCAGCCTGGAAGATCATCGGCAAAGCCAGCCCACACAGCTACCAGATGTGGCGGACAGGATCTCCCCAG
  TGACTCAGTATCCGGTCACCCCCCAGATGCTAAGCCCTAAAGCGTTTCCGGGCAATTCTCCTGCTACCCC
  ACGGAGGCCACCTCAGGACTGGACCCAAACAGCTATGCCAACCTCTGAGGGTCAAGGACAGTCCAGGGAG
  GAACCAGGGCATGGAGGCACCAGCCTTCCCGCCACCTCCCCAGTGACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221535 representing NM_001081127
  Red=Cloning site Green=Tags(s)
  
  MAWLRALLHCLLPWYCALCAAAGSQTPDLRLSGKLHDYVVTVPCSTDFQGRFLSHVVSAPAAPSPRSHLR
  VARSPLSLERETPRPGGPRQHFLYFNVTVFGKLLHLRLQPNRRLVAPGAPVEWQEDFRELFRQPLQQECV
  YTGGVTGMPGAAVAISNCDGLAGLIRTDNSDYFIEPLERGQQEKEAGGRTHVVYRREAVQREWKEPHGDL
  HNEAFGLGDLPNVLDLVGDRLGDAERKRRHAKPGSYSIEVLLAVDDSVVRFHGREHTQNYVLTLMNIVDE
  IYHDESLGAHVNIALVRLIMVGYRQSLSLIERGNPARSLEQVCRWAHSQQRQDPSHTEHHDHVIFLTRQN
  FGPSGYAPVTGMCHPLRSCALNHEDGFSSAFVVAHETGHVLGMEHDGQGNGCDDETSLGSVMAPLVQAAF
  HRFHWSRCSKLELSRYLPSYDCLLDDPFERTWPQPPELPGIDYSMDEQCRFDFGTGYHTCLAFRTFEPCK
  QLWCSHPDNPYFCKTKKGPPLDGTECAPGKWCFKGHCIWKSPEQTYGQDGGWSSWTNFGSCSRSCGGGVR
  SRSRSCDNPPPAYGGRPCSGSMFEYQICNSEDCPGPYEDFRAQQCAKRNSYYTHQDAKHSWLPYEPDSDA
  QKCELICQSADTGDVVFMNQVVHDGTRCSYRDPYSVCARGECVPFGCDKEVGSMKTDDKCGVCGGDNSHC
  RTVKGTLGKGSKQAAALKQVQIPAGARHIQIELLEKAPHRIAVKNQVTGSFIFNPKGKEASSRTFTALGL
  EWEHEAEDTKDSLRTNGPLPEAIAILVLPPAEGKPRGSLAYKYVIHEDLLPLIGSNNVLLEETDTYEWAL
  KSWSPCSKACGGGIQFTKYGCRRRRDHHMVHRHLCDHKKRPKPIRRRCNQHSCPQPTWVTEEWGACSRSC
  GKLGLQTRGVQCLLPLSNGTHKAMPAKACLGNRPEAKKPCLRVPCPAQWRTGAWSQCSATCGEGIQQRQV
  VCRNTSSALGPCEGVKPDMVQICSLPACGGDLQNSTVKAEVQDPVTKTGYREPQSRPLTPEDRISTMEPC
  VRDRSVFCRMEVLDRYCTIPGYHRLCCESCIKKTSGPNASLALPPTFSTPGSLLPAPKATLEDVKSTRGP
  TSLEDHRQSQPTQLPDVADRISPVTQYPVTPQMLSPKAFPGNSPATPRRPPQDWTQTAMPTSEGQGQSRE
  EPGHGGTSLPATSPVT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081127
• ORF Size: 3618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081127.2
• RefSeq Size: 5188 bp
• RefSeq ORF: 3621 bp
• Locus ID: 237360
• Cytogenetics: 10 B4