Cdc42bpb (NM_183016) Mouse Tagged ORF Clone

CAT#: MG221283

Cdc42bpb (tGFP-tagged) - Mouse CDC42 binding protein kinase beta (Cdc42bpb), (10ug)

Product Images

Circular map for MG221283

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc42bpb
• Synonyms: MRCKb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221283 representing NM_183016
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCAAGGTGCGGCTCAAGAAGCTGGAGCAGCTGCTCCTGGACGGGCCGTGGCGCAACGATAGCG
  CCCTGAGCGTGGAGACGCTGCTGGACGTGCTGGTGTGCCTTTACACCGAGTGCAGCCACTCGGCGCTGCG
  CCGCGACAAGTATGTGGCGGAGTTCCTCGAGTGGGCCAAGCCTTTCACCCAGCTCGTGAAGGACATGCAG
  CTTCATCGAGAAGACTTCGAGATCATCAAAGTGATCGGGAGAGGAGCCTTTGGTGAGGTTGCTGTTGTCA
  AAATGAAGAACACTGAACGAATTTATGCAATGAAAATTCTCAACAAATGGGAAATGCTAAAGAGAGCAGA
  GACAGCTTGCTTTCGAGAAGAGCGTGACGTGCTGGTGAACGGCGACTGCCAGTGGATCACGGCTCTGCAC
  TATGCCTTTCAGGATGAGAACTACCTGTACTTGGTCATGGATTACTATGTAGGTGGTGATCTGCTGACCC
  TGCTGAGTAAGTTTGAAGACAAGCTTCCGGAAGACATGGCGAGGTTCTACATTGGCGAGATGGTGTTGGC
  CATTGACTCGATCCACCAGCTCCACTATGTGCACAGAGACATCAAGCCCGACAACGTCCTTCTAGATGTG
  AACGGTCACATCCGCCTGGCTGACTTTGGCTCGTGCTTGAAGATGAACGATGATGGCACTGTTCAGTCTT
  CCGTGGCCGTGGGCACACCTGACTACATCTCACCAGAGATCCTGCAGGCCATGGAGGATGGCATGGGCAA
  ATACGGGCCCGAGTGTGACTGGTGGTCGCTGGGCGTCTGCATGTATGAGATGCTATACGGAGAAACCCCG
  TTCTACGCAGAGTCTCTGGTGGAGACATACGGGAAGATCATGAACCACGAGGAGCGGTTTCAGTTCCCAT
  CCCATGTCACCGATGTCTCTGAAGAAGCAAAAGACCTTATCCAGAGACTAATATGCAGCAGAGAGCGCCG
  ACTTGGGCAGAATGGAATAGAAGACTTTAAGAAACACGCATTCTTTGAAGGTCTGAATTGGGAGAATATA
  CGAAACCTTGAAGCGCCCTACATTCCCGATGTGAGCAGCCCTTCGGACACGTCCAACTTCGATGTGGACG
  ACGACATGCTGAGAAACATTGAAATCTTACCTCCCGGCTCTCACACGGGCTTCTCGGGACTGCATTTGCC
  CTTCATCGGTTTCACATTCACGACGGAAAGCTGCTTTTCTGACCGGGGCTCTCTGAAGAGCATGACTCAG
  TCTAACACGCTAACCAAAGACGAAGATGTGCAGCGGGACTTGGAGAACAGCTTGCAGATCGAAGCGTACG
  AGCGAAGGATACGGAGGCTGGAGCAGGAGAAGCTGGAGCTCAGCCGGAAGCTGCAAGAATCCACCCAGAC
  TGTGCAGTCCCTTCACGGTTCCACACGGGCCCTGGGCAACTCAAACCGCGACAAGGAAATCAAGAGGCTG
  AATGAAGAGCTTGAACGCATGAAGAGTAAAATGGCAGATTCAAACAGGCTCGAACGCCAGCTGGAGGACA
  CAGTGACACTTCGCCAGGAGCATGAGGACTCCACACACCGGCTGAAGGGCCTCGAGAAGCAGTACCGCCT
  GGCCCGGCAGGAGAAGGAAGAATTGCACAAGCAATTGGTTGAGGCTTCAGAGCGATTGAAATCCCAGACC
  AAAGAACTTAAAGACGCGCATCAGCAGCGAAAACGGGCCCTGCAGGAGTTCTCAGAGCTCAATGAGCGCA
  TGGCGGAGCTCAGGTCGCTGAAGCAGAAGGTGTCCCGTCAGCTCCGGGACAAGGAGGAGGAGATGGAGGT
  GGCCATGCAGAAGATCGACTCCATGCGGCAGGACCTCCGCAAGTCTGAGAAGTCCAGGAAAGAGCTGGAA
  GCTCGGCTTGAGGACGCAGCTGCCGAGGCCTCTAAGGAGCGGAAGCTCCGAGAACACAGCGAGAGCTTCT
  GTAAGCAGATGGAGCGCGAGCTCGAGGCCCTCAAGGTAAAGCAAGGAGGCCGGGGGCCAGGGGCCGCGTC
  GGAACATCAGCAGGAGATCTCCAAAATCAGGTCAGAGCTCGAGAAGAAAGTCTTGTTCTACGAGGAGGAG
  CTGGTGCGCCGAGAGGCCTCCCACGTGCTAGAAGTAAAGAACGTGAAGAAGGAAGTCCACGACTCCGAAA
  GCCACCAGTTGGCCCTGCAGAAAGAGGTCCTGATGCTGAAAGACAAGTTAGAAAAGTCAAAGCGAGAACG
  GCACAGTGAGATGGAGGAGGCCATAGGCACCGTGAAGGACAAGTACGAACGGGAGAGGGCCATGCTGTTC
  GATGAGAACAAGAAGTTAACAGCAGAAAACGAAAAGCTTTGTTCCTTTGTGGATAAACTAACAGCCCAAA
  ACAGACAGCTGGAAGATGAGCTGCAGGATCTGGCATCCAAGAAGGAGTCAGTTGCGCACTGGGAGGCGCA
  GATCGCAGAGATCATTCAGTGGGTCAGCGATGAGAAAGATGCCAGAGGCTACCTTCAAGCCCTTGCTTCT
  AAGATGACCGAAGAGCTTGAGACCTTGCGAAGTTCTAGTTTGGGATCCAGGACACTGGATCCACTCTGGA
  AAGTTCGTCGGAGTCAGAAGCTGGACATGTCTGCACGGCTGGAATTGCAGTCTGCTCTTGAGGCCGAGAT
  CCGTGCCAAACAGCTCGTTCAGGAGGAGCTGAGGAAGGTCAAAGACAGCAGCCTGGCCTTCGAAAGCAAA
  CTGAAGGAATCGGAAGCGAAAAACAGGGAATTGTTAGAAGAAATGCAAAGTCTGAGGAAGAGGATGGAAG
  AGAAGTTTAGAGCGGATACAGGGCTCAAACTTCCAGATTTCCAGGATTCTATCTTTGAGTATTTCAACAC
  TGCTCCTCTTGCACATGATCTGACTTTTAGAACCAGCTCAGCTAGTGACCAGGAAACACAGGCTTCAAAG
  ATGGACTTGTCCCCATCAGTGTCTGTAGCCACGAGCACAGAGCAGCAGGAGGATATGGCTCGGCCACAGC
  AGAGGCCGTCTCCTGTGCCGCTGCCCAGCACGCAGGCCCTCGCCATGGCTGGACCGAAGCCCAAAGCCCA
  CCAGTTCAGCATCAAGTCCTTCCCTAGCCCCACCCAGTGCAGCCACTGCACGTCCTTGATGGTCGGACTG
  ATCCGACAGGGCTACGCCTGTGAGGTCTGTGCATTTTCCTGCCATGTGTCCTGCAAAGACAGCGCTCCCC
  AGGTGTGCCCCATACCTCCTGAGCAGTCCAAGAGGCCTCTTGGCGTAGACGTGCAGAGGGGCATAGGCAC
  GGCCTACAAGGGCTACGTCAAGGTCCCAAAGCCCACAGGCGTGAAGAAAGGATGGCAGAGGGCTTACGCC
  GTGGTCTGTGACTGCAAACTCTTCCTGTACGACCTGCCAGAAGGGAAGTCGACCCAGCCCGGTGTCGTTG
  CCAGTCAAGTCTTGGACCTCAGAGATGAGGAGTTTGCTGTGAGTTCAGTCCTGGCCTCAGATGTTATCCA
  TGCTACACGCCGAGACATTCCGTGCATATTCAGGGTGACGGCCTCTCTCTTAGGTTCGCCTTCTAAGACC
  AGCTCACTGCTCATCCTGACGGAGAACGAGAATGAAAAGAGGAAGTGGGTAGGGATCCTTGAAGGGCTGC
  AGGCCATCTTGCACAAGAACCGGCTGAAGAGCCAGGTAGTGCACGTCGCACAGGAGGCCTACGACAGCTC
  GCTGCCGCTCATCAAGGCCGTCCTGGCTGCTGCTATCGTGGATGGAGACAGGATTGCGGTCGGCCTGGAA
  GAAGGGCTCTACGTCATTGAGCTCACCCGAGACGTGATCGTCCGCGCTGCTGACTGCAAGAAGGTGTACC
  AGATCGAGCTGGCGCCCAAGGAGAAGATCGCCATCCTCCTGTGTGGCCGGAACCACCATGTGCACCTCTA
  CCCCTGGTCCTCCTTCGACGGAGCAGAAGCGAGCAACTTTGACATCAAGCTCCCGGAAACAAAGGGCTGC
  CAGCTCATAGCGACAGGGACGCTGAGGAAGAGCTCGTCCACCTGCCTGTTTGTCGCTGTGAAGCGACTAA
  TCCTTTGCTACGAGATCCAGAGAACTAAGCCTTTCCACAGGAAGTTCAGTGAGCTGGTGGCTCCGGGACA
  CGTGCAGTGGATGGCCGTGTTCAAGGACAGGCTCTGTGTTGGCTACCCCTCTGGGTTCTCTCTGTTGAGC
  ATCCAGGGGGACGGGCCGCCTCTCGACCTGGTAAATCCCACTGACCCCTCGCTCGCGTTCCTCTCACAGC
  AGTCTTTCGATGCCCTCTGTGCTGTGGAGCTCAAAAGTGAGGAGTACCTGCTTTGCTTCAGCCACATGGG
  ACTGTACGTGGACCCTCAAGGTCGGAGGTCACGCATGCAGGAGCTCATGTGGCCTGCGGCTCCTGTCGCC
  TGTAGTTGCAGCCCCACCCATGTCACAGTGTACAGCGAATACGGGGTGGATGTCTTCGACGTGCGCACCA
  TGGAGTGGGTTCAGACCATCGGCCTGCGGAGGATAAGACCTCTGAACTCTGATGGCAGCCTCAACCTGCT
  GGGCTGTGAGCCCCCTCGCCTCATCTACTTCAAAAACAAGTTCTCAGGAACAATCCTCAATGTGCCCGAC
  ACCTCGGACAACAGCAAGAAGCAGATGCTGAGGACACGGAGCAAACGGCGTTTTGTCTTCAAGGTTCCCG
  AGGAAGAGCGGCTACAGCAGCGGCGAGAGATGCTCAGAGACCCCGAACTGCGATCCAAAATGATATCCAA
  CCCAACCAACTTCAACCACGTGGCTCACATGGGTCCTGGGGATGGCATGCAGGTGCTCATGGACCTGCCT
  CTGAGTGCTGCACCCACTGTCCAGGAGGAGAAGCAGGGCCCTACCCCAGCAGGCCTCCCCCGGCAGCCGC
  CATCCAGGAGCAAGCCCTATGTCTCGTGGCCGTCGTCAGGTGGGTCCGAGCCTGGAGTGCCTGTGCCTCT
  GAGGAGCATGTCCGACCCCGACCAGGATTTTGACAAAGAGCCTGACTCTGATTCCACCAAACACTCAACT
  CCATCCAATAGCTCCAACCCTAGCGGCCCCCCAAGCCCCAACTCGCCCCATCGGAGCCAGCTCCCTATGG
  AAGGCCTGGACCAGCCATCCTGTGACGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221283 representing NM_183016
  Red=Cloning site Green=Tags(s)
  
  MSAKVRLKKLEQLLLDGPWRNDSALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKDMQ
  LHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALH
  YAFQDENYLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDV
  NGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETP
  FYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENI
  RNLEAPYIPDVSSPSDTSNFDVDDDMLRNIEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSMTQ
  SNTLTKDEDVQRDLENSLQIEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSTRALGNSNRDKEIKRL
  NEELERMKSKMADSNRLERQLEDTVTLRQEHEDSTHRLKGLEKQYRLARQEKEELHKQLVEASERLKSQT
  KELKDAHQQRKRALQEFSELNERMAELRSLKQKVSRQLRDKEEEMEVAMQKIDSMRQDLRKSEKSRKELE
  ARLEDAAAEASKERKLREHSESFCKQMERELEALKVKQGGRGPGAASEHQQEISKIRSELEKKVLFYEEE
  LVRREASHVLEVKNVKKEVHDSESHQLALQKEVLMLKDKLEKSKRERHSEMEEAIGTVKDKYERERAMLF
  DENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALAS
  KMTEELETLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDSSLAFESK
  LKESEAKNRELLEEMQSLRKRMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASDQETQASK
  MDLSPSVSVATSTEQQEDMARPQQRPSPVPLPSTQALAMAGPKPKAHQFSIKSFPSPTQCSHCTSLMVGL
  IRQGYACEVCAFSCHVSCKDSAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGYVKVPKPTGVKKGWQRAYA
  VVCDCKLFLYDLPEGKSTQPGVVASQVLDLRDEEFAVSSVLASDVIHATRRDIPCIFRVTASLLGSPSKT
  SSLLILTENENEKRKWVGILEGLQAILHKNRLKSQVVHVAQEAYDSSLPLIKAVLAAAIVDGDRIAVGLE
  EGLYVIELTRDVIVRAADCKKVYQIELAPKEKIAILLCGRNHHVHLYPWSSFDGAEASNFDIKLPETKGC
  QLIATGTLRKSSSTCLFVAVKRLILCYEIQRTKPFHRKFSELVAPGHVQWMAVFKDRLCVGYPSGFSLLS
  IQGDGPPLDLVNPTDPSLAFLSQQSFDALCAVELKSEEYLLCFSHMGLYVDPQGRRSRMQELMWPAAPVA
  CSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSDGSLNLLGCEPPRLIYFKNKFSGTILNVPD
  TSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLP
  LSAAPTVQEEKQGPTPAGLPRQPPSRSKPYVSWPSSGGSEPGVPVPLRSMSDPDQDFDKEPDSDSTKHST
  PSNSSNPSGPPSPNSPHRSQLPMEGLDQPSCDA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183016
• ORF Size: 5139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183016.2, NP_898837.2
• RefSeq Size: 6718 bp
• RefSeq ORF: 5142 bp
• Locus ID: 217866
• UniProt ID: Q7TT50
• Cytogenetics: 12 60.94 cM
• Gene Summary: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function]