Plekhg4 (NM_001081333) Mouse Tagged ORF Clone

CAT#: MG221235

Plekhg4 (tGFP-tagged) - Mouse pleckstrin homology domain containing family G (with RhoGef domain) member 4 (Plekhg4), (10ug)

Product Images

Circular map for MG221235

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plekhg4
• Synonyms: 4931414L13Rik; AI448583; mFLJ00068
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221235 representing NM_001081333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCTCTTGAGTGTGGGGATGTGTCCCCAGACTCCCAGGGCCATGTTGCAGATGGGAAGAGAT
  TTGCTGTGTGCAGTTTCAGGAACACCCGGAAGGAGGAGGAACCTGCTCACGAGATACAGGCTATGGACCC
  AAGACCTTCAAGACCAGCAGCAGGGGCAACCCAAGGCGTAGGGCTACAGGGGGATTTCTTGGCCAAGGAG
  CCTCAATCGCTTCCGGAACAAAGGGCTGAGGATGGGCCACGGGGCTACCAGAGGAGCATAATAGGAGCCC
  CCCCAACCCAGTCAGAGGAGCCAGCCTTCTCTGAATTGGAAAACCTCTTGTGTTCTGGGTCCTCTCACCT
  TAACCTGACACAGGGTGACAATGACTATCGAGGGGAAGGCTTGATAGGGGACCCAGGTCCAGGCAGAACG
  CTGCCAGCTGGCTGTAGCCCTTTATCAGAGACATCACCAAAGCTACTGGAGGCAGACCCTAGTGGATCCA
  GCTTCCCTAAGCCTGCTGACTGTCTCCTAGCTCGAGACCTCGCCTGGGAGCTACTGGCCAGTGGCATGGC
  TGCCCTACCAGGGACACGGGATGTCGAAGGCCGAGCGGTGTTGCTTCTGTGTGCCCACAGCCCAGCTTGG
  CTCCATCCCAAGTCTAATAGCCATGAGCTGCTGAGCCTTTTGTTTTACCTGCGAGGCATCCCCAGGCCTG
  AAGTGCAGGCACTGGGCCTGACCGTACTAGTCGATGCCCGAGTCTGCTCGCCGAGCTCTTCCCTCACCTG
  GGGCCTCAGCCGGCTACAGGAAGCATCCCCGGGGTCTGTACAACAAGTGCTGCTCATTGGAAAGATGCCA
  GAGGACGTGCCTGTCGGGCTTCAGTGGAAGCAGCTACGTTCTCACCAGAGCCTGCTGACCCACATTCCTA
  ACGTGGAGTTGCCTACTTCGCTAGGAGGGAGCCTGTCTTACTGCCACCAAGGCTGGCTGGATTTCCGGAT
  GCGTCTGGAAGCCCTGCAGCAGAGTTGCCAGGTGGCTTGTGCCCTGCTTCAGGGTGTCATTGACAGTATG
  AAGGCACTGCGGCCACCCATGGAGTCTGGAGAAGTCGCTCAGCTGCTACAGCAGACACAGTCCCTCATGC
  AGCAGGTATTAGATTCGCCACAGTTGTCATGGTTACAAAGCCAGGGAAGCCAGGAGCTGGCATGGTTGAA
  GCAAGGGACGCCCCGGGTGACCCTGAGCCCCGATTACAGGTCTGCAGTAGACAAGGCTGATGCGCTCTAC
  GGTCAGGTGGATGAACTTTTGCATCAGCTAGCCCTGAAGAGCAACCAGCGAATCCGGGCCCTCGAGCTCC
  TGCAAGCGCTGGAAGCCCAGGAGGGTGTGCTGCGCCAGATTGAAGTATGGTTACAGGAGGTGGGTTGGCC
  AGGAGTGGAGGAACCAGGGGAGCCCTCGCTCGACACGCTGCTCCAGGCTCAAGGCCCTTTTCAAGAGCTG
  GACCTGGTTGCCCAGGAACAGGTCACGCAAGGGGAGAAGTTACTGCAGCCCCTGGTTGGCTTGGAGGCTG
  CTGAACTGGGGTCCCCTGGAAAGCGTTTTCTGGCCCTGAGATCCCAGCTGACGGAATTTTCCAGAGCTTT
  GGCCCAGCGGCGCCAGCGGTTGGCAGATGCTGAGAAGCTCTTTCAGTTCTTCAAGCAGGCCTCGACGTGG
  ACCGAGGAGGGGCGGCGGCTACTGACAGAGCTGGAGCAGGAGCGCCCAGAGGTGGTGCTGCAGCGGCTGC
  AGCTGCACTGGACCAGACACCCTGACTTGCCCCCTGCCCACTTCCGGAAAATGTGGGCTCTGGCCACAGG
  CCTGGGCTCTGAAGGCATCCGCCAGGAATGTCGCTGTGCCTGGGCACAGTGCCAGGACACCTGGTTGGCT
  CTGGACCAAAAGCGTGAGGCTGCACTGAAGCCGATGCCAGCAACGAACAACACCGCTACTTTGTATGTCA
  GGCGGGCTCCTGCTGTACCCACTGTCCCTCCACTGCGGAAGGCCTACAGCTTCGACCGGAATCTGGGGAG
  GCATCTCCAAGATGTTTCCAGTCGTGGCCACTGTGCAGCCATTGTTACTGACTGCCACAGACCTGAGGCC
  AGAGGAGGTGTCCGGCCCAGGTCGTCTCCTCCTGTGCCTCTTTCAGGCAGCTCTGACTTCAGGAGCCCCA
  ACAGGCTACAGCTAGTATTGGCGGAGATGGTGGCCACAGAGCGTGAGTATGTCCGGGCCCTTGACTATAC
  CATACAGAACTACTTCCCTGAGTTAGATCGACCCGATGTGCCCCAGGGCCTCCGTGGCCAGCGTGCGCAC
  CTCTTTGGCAACCTTGAAAAGCTTCGGGACTTCCACTACAATTTCTTCCTGCGTGAGCTAGAGGCTTGCA
  CCCGGCACCCACCTCGAGTGGCTCATGCCTTCCTGCGCCATAGGGTGCAGTTTGGAATGTATGCCCTCTA
  CAGCAAGAACAAACCTCGTTCTGATGCTCTGATGACAAACTATGGTCACACCTTCTTTAAGGAGAAGCAG
  CAAGCACTGGGGGACCACCTGGACTTGGCTTCCTACCTACTGAAGCCCATCCAGCACATGAGCAAGTATG
  CCTTACTGCTGCAGGAGCTGGCAAGGGCCTGTGGGGGCCCAGCGCAAGAGCTGGGTGCCCTCCAGGCAGC
  CCAGAGCCTTGTGCACTTCCAGCTGAGACACGGCAATGACCTGCTAGCCATGGATGCCATCCAGGGCTGT
  GACGTAAACCTCAAGGAACAGGGCCAGTTGGTACGGCAGGATGAGTTCACAGTGCGGGCTGGGCGCCACA
  AAGCCTGTCGTCGCGTTTTCCTGTTTGAGGAGCTGCTGCTCTTCAGCAAGCCCCGCCGTGGGCCTGCAGG
  GGTTGACATATTCACCTACAAGCGATCCTTCAAAATGGCAGACCTCGGCCTCACTGAGTGCTGTGGGGAA
  AGCCAACTGCGCTTTGAAATCTGGTTCCGACGACGCAAGGCCAGGGACCTGTTTGTGCTGCAGGCCTCCG
  ATGTGGCCACCAAACAAGCCTGGACAGCCGATATCTCCCGTCTCCTGTGGAGACAGGCTGTCCATAACAA
  GGAGGTGCGCATGGCTGAGATGGCATCCATGGGTGTGGGGAGCAAAGCCTTCTGGGACATTGCCCCCAGT
  GAGGAAGCTATCAATGACCGCAACATCAACTATGTGCTGAAGAGACGAGATGTTCGCTCTAGGGCCTCCA
  TTGCTGTGGCCCCATTTGACTGTGACAACCCTTATCTGGGTGCCTTGGGCTCCCTTCCTGGAGACCGTGC
  CTCTGGCTCTGTTCTGGGCTCTCTTAATCTACACCTGTACAGAGATCCAGCTCTTATGGGTGGTCACTGG
  TCCTTGTATCCACCCAACTTCTCAGAGGAAGCATCACTAGATTTGGGCAGCCAGCCTTCTTTGACTCCCG
  AGGACTCAGAGGTCTCATCCCAGTGCCCATCAGCCAGTGGCTCTAGTGATTCTGACAGCAGCTGTGTGTC
  AGGGCAGACCATGGGCAGAGGCCTTGAGGATTTGTCCTGTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221235 representing NM_001081333
  Red=Cloning site Green=Tags(s)
  
  MEGPLECGDVSPDSQGHVADGKRFAVCSFRNTRKEEEPAHEIQAMDPRPSRPAAGATQGVGLQGDFLAKE
  PQSLPEQRAEDGPRGYQRSIIGAPPTQSEEPAFSELENLLCSGSSHLNLTQGDNDYRGEGLIGDPGPGRT
  LPAGCSPLSETSPKLLEADPSGSSFPKPADCLLARDLAWELLASGMAALPGTRDVEGRAVLLLCAHSPAW
  LHPKSNSHELLSLLFYLRGIPRPEVQALGLTVLVDARVCSPSSSLTWGLSRLQEASPGSVQQVLLIGKMP
  EDVPVGLQWKQLRSHQSLLTHIPNVELPTSLGGSLSYCHQGWLDFRMRLEALQQSCQVACALLQGVIDSM
  KALRPPMESGEVAQLLQQTQSLMQQVLDSPQLSWLQSQGSQELAWLKQGTPRVTLSPDYRSAVDKADALY
  GQVDELLHQLALKSNQRIRALELLQALEAQEGVLRQIEVWLQEVGWPGVEEPGEPSLDTLLQAQGPFQEL
  DLVAQEQVTQGEKLLQPLVGLEAAELGSPGKRFLALRSQLTEFSRALAQRRQRLADAEKLFQFFKQASTW
  TEEGRRLLTELEQERPEVVLQRLQLHWTRHPDLPPAHFRKMWALATGLGSEGIRQECRCAWAQCQDTWLA
  LDQKREAALKPMPATNNTATLYVRRAPAVPTVPPLRKAYSFDRNLGRHLQDVSSRGHCAAIVTDCHRPEA
  RGGVRPRSSPPVPLSGSSDFRSPNRLQLVLAEMVATEREYVRALDYTIQNYFPELDRPDVPQGLRGQRAH
  LFGNLEKLRDFHYNFFLRELEACTRHPPRVAHAFLRHRVQFGMYALYSKNKPRSDALMTNYGHTFFKEKQ
  QALGDHLDLASYLLKPIQHMSKYALLLQELARACGGPAQELGALQAAQSLVHFQLRHGNDLLAMDAIQGC
  DVNLKEQGQLVRQDEFTVRAGRHKACRRVFLFEELLLFSKPRRGPAGVDIFTYKRSFKMADLGLTECCGE
  SQLRFEIWFRRRKARDLFVLQASDVATKQAWTADISRLLWRQAVHNKEVRMAEMASMGVGSKAFWDIAPS
  EEAINDRNINYVLKRRDVRSRASIAVAPFDCDNPYLGALGSLPGDRASGSVLGSLNLHLYRDPALMGGHW
  SLYPPNFSEEASLDLGSQPSLTPEDSEVSSQCPSASGSSDSDSSCVSGQTMGRGLEDLSCV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081333
• ORF Size: 3543 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081333.2
• RefSeq Size: 4167 bp
• RefSeq ORF: 3546 bp
• Locus ID: 102075
• Cytogenetics: 8 D3