Catsperg1 (NM_001164658) Mouse Tagged ORF Clone

CAT#: MG221221

Catsperg1 (tGFP-tagged) - Mouse cation channel sperm-associated gamma 1 (Catsperg1), (10ug)

Product Images

Circular map for MG221221

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Catsperg1
• Synonyms: A230107C01Rik; Catsperg
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221221 representing NM_001164658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCCGGCCAGCCATGTCCCCGGTCAGCCCTGTGTGGCCGAGAAAACCAAACCTTTGGGCATTCT
  GGGTACTGCGGCTAGTGCTATTGCTGTCGTTGAAGTCGTGGGCGGAAGATACCTTACAGCATTGCACCTG
  GCTTCTTGTCCTGAACAAGTTCGAGAAGGTAGGCTTGCATCTCTCCAAGGATCGATTTCAAGATCATGAG
  CCCATCGATACAGTGGCCAAGGTGTTCCAAAAGTTGACAGACTCGCCAATCGATCCGTCAGAGAACTATT
  TGAGCTTTCCTTACTACTTGCAAATCAACTTTTCCTGTCCGGGACAGAATAGTGAGGAGCTGGCCCGAAA
  GGGCCACCTGATGGGGATGAAGCCCATGGTCCGGATCAACTACATGTACTCAGTCAATTTCTACCGTTGG
  GAGATGGAAAATTTGCAGATTCTGATGGAGGCTGCTCCAATGCGCAGCACAGGGTACTGCCCGGCAGAGG
  CCATGTGCATCCTGAACTGGTACACACCCATGCCCTTTAAAAACGGCTCCGTGGTCTCCTCAGTGGACAT
  CTATACCAATGGTATAGGGCCCTTCATACCCAAGAAAAGGTTCTATGTGAATATGAATGGCTTCTTGAAG
  AGAGATGCAAGTGGTAAATCCTTATTCGCCATAGGATATGAGAGCCTTGTTCTGAAGTCGAGTCACTTTA
  GGCTTTCAAAATCAAGACCCCTGTGGTACACTGTGAACCATGCCCCCGTGTTCATCCTTGGAGGCTTTTA
  TGATGAAAAGGCCATCCTGTTCTCAGATTCTAATTTCCAGGACTATGTTCTCTTGGAGCTGAGTATTGAC
  AGTTGCTGGGTCGGCTCCTTCTACTGTCCCATTCTTGGCTTCTCAGCCACCATCCATGACGCCATTGCCA
  CCGAGAGCACACTCTTCATTCGACAGAACCAGCTTGTCTACTATTTCACAGGCACCTACAGCACGCTCTT
  TGACAAAAGCCATGGCAGTAGCAGATGGGTTCGTGTCCTGCCTAGCGAGTGCATCAAGAGACTGTGCCCG
  GTGTATTTCAGTGGCAATGGTTCTGAGTACGTCCTAGCCCTCACCACTGGCAAGAATGAGGGTTATATTC
  ACATCGGGACCATCACAGATGGCCTCGTGTCCTTCGAGATGGTGCCAGATGGCTGGTCAGTATGTGAAAA
  ATTACCAGGTAAAAACTGCAGCATCGACTGGGCTACATACATCACTGATGAGAGGAACCTGCTGTTGTTA
  GTGAAAATTGACTCTGGGCAATTTTACCTAGTCAACTTTAATACAGAGTTCAAGACTCTGAATATCCTCT
  ACAAAATACCAGAGTTTATACCGGAAGCTAAGGAACTAGACTTCTTGGTGCTCCTTGATACAGTGACATA
  CACCAACACTCCTATGACACCCAAGGGCTTGTTCTTCAACACATTGAACAACATGTTGTACATCTGGGGC
  AACTTCATCCTGCAAAGCTACAATAGGGAAGAATTCATTTTTCTGGCGGACTTCCCCAAGGAGTCCACCA
  TCAAATACATGGTCAACTCTTTCAAAGGACAAATGGCTGTTGTCACAGAAAATGAAGAGCTAGTCTATCT
  TTTTGACGTTGGAAAAGAACGTTTAGTCAAGAGGCTCCTGCCTGTGGGAACACTGATGGAGTACAACCTG
  CCCAAACCCTTCACAGTGGTGAACCAAGGGAACTACAAGATGATCACCTTCACCAACACGTGCCCTTTTA
  AGGCCATTCATGCAGTTGATGTGCCCAAAAAACAGCATGCATCACGCACAGAGAGCTATGTTGCTCTGCC
  ACCTCTGGTCTCTGAATCCTTGGGCTTCCACAACAATAACACACTCGCTGTCTATCAAGGCCTCGTCTAT
  TACCTGCTGTGGCTGCACTCCAAGTATGATAAGCCCTATGCAGACCCAGTGCACGATCCCACCTGGCGCT
  GGTGGCAACACAAAACAAAGGACAAGGATTACTTCTTTTACCTGTTCAGCAACCGGCTGGCGGCAGAAGG
  CATATACATCAACATGAACGCCTACCAAAAGCTCTACAACATGTCGGGTGACTATGGAATACCCGATCTT
  TTCTTCCTAGACAAGGGCAACTGGTTCACCTTCACTGTGGTCTTATTGTCACATCAGGATACCTTCACAA
  GCTCTGATAGCCAAGGACCCACTATTAATGTGGACAAGAAGCTGTCATTGTCTCTGGTACTGGCGGATCC
  TGAGTGCCTTTCAGTAACTGCGACTCGGGAGTTCCTTCTTAATCGCAACACTCTGCTCACCAAGATTAAA
  GTCATAGATAAAAAGAGATGCTCCGAACAGGGCATGGTTGGACGTAACATCAAGAAGACTTCCATGTTGA
  TCAAGGTCTTGGGCGCTCCTGGGAATTGCATCCAGCGCACGTACCTTGGGGACCACATTCAAGGTATCAG
  GCTGGTACCAATATTCATCGGCTGTCCTCCTGGCAAGCGCTTGGCCTTTGATGTCTCCTACACGATTAAG
  CATTCTGAGGAGATAAACAAGCACTATTTCGACTGTGTGATTAAAGACGCCGAGATGCCATGCTTCCTCT
  TTCGTGACTTATTCCAGCCCTTCTTCTTAGTCCAAGACTTGGTGACTGGCGACTCTGGCAGTTTCTTGGG
  CAGTTACGTGCTGAAGGTGGTGGGTGGTGGTCGCACACTGAACACCATCAGAGACTACACGGAGGAAGAA
  ATCTTCCGTTACAACAGCCCGCTGGACACAACGAATAGCCTCATCTGGAAAACAAAGGTCGAAAGGACCA
  CGGAGGACAAAAAGTTCTACATAATGTCCCATGAGAGCCCTGGAGTCGAGTGGCTGTGCTTGGAGAACTC
  CCCTTGCTATGACATCATCCCCCAAAGCATCTACCCACCTGAGTTCTTCTTCAAGTTGTTAGTGAGCAAT
  AGAGGTGTAGACAATAGCACATACTGTGACTACAAGCTCACCTTTATAGTGCACATCCATGGGCTACCGC
  TCAGCAGCAAGCGGTCCTCCTTCATTGTTATGGTGTCTACCAGCTTCTTTATAGCCCTGGTTGTCTTCTA
  CATCCTGTTCTGCCTCGTGTGGCCTCACATAGTGAAGGCCTGGGTCTCATTTCGCTGGAAGATTCACAAC
  ATGATGGCACCAGAGACCTATTCATCTTCCTCCTCTTCTGGGGGCTTCACCCTCCATTCTCACAGCTCAG
  AGGGAAGTTTTGAGGGTCCCTCCAGACCAGGTACCAAAGAGGACAATGTTCAAGCTAAGAGAGCAAAGGT
  GGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221221 representing NM_001164658
  Red=Cloning site Green=Tags(s)
  
  MVSRPAMSPVSPVWPRKPNLWAFWVLRLVLLLSLKSWAEDTLQHCTWLLVLNKFEKVGLHLSKDRFQDHE
  PIDTVAKVFQKLTDSPIDPSENYLSFPYYLQINFSCPGQNSEELARKGHLMGMKPMVRINYMYSVNFYRW
  EMENLQILMEAAPMRSTGYCPAEAMCILNWYTPMPFKNGSVVSSVDIYTNGIGPFIPKKRFYVNMNGFLK
  RDASGKSLFAIGYESLVLKSSHFRLSKSRPLWYTVNHAPVFILGGFYDEKAILFSDSNFQDYVLLELSID
  SCWVGSFYCPILGFSATIHDAIATESTLFIRQNQLVYYFTGTYSTLFDKSHGSSRWVRVLPSECIKRLCP
  VYFSGNGSEYVLALTTGKNEGYIHIGTITDGLVSFEMVPDGWSVCEKLPGKNCSIDWATYITDERNLLLL
  VKIDSGQFYLVNFNTEFKTLNILYKIPEFIPEAKELDFLVLLDTVTYTNTPMTPKGLFFNTLNNMLYIWG
  NFILQSYNREEFIFLADFPKESTIKYMVNSFKGQMAVVTENEELVYLFDVGKERLVKRLLPVGTLMEYNL
  PKPFTVVNQGNYKMITFTNTCPFKAIHAVDVPKKQHASRTESYVALPPLVSESLGFHNNNTLAVYQGLVY
  YLLWLHSKYDKPYADPVHDPTWRWWQHKTKDKDYFFYLFSNRLAAEGIYINMNAYQKLYNMSGDYGIPDL
  FFLDKGNWFTFTVVLLSHQDTFTSSDSQGPTINVDKKLSLSLVLADPECLSVTATREFLLNRNTLLTKIK
  VIDKKRCSEQGMVGRNIKKTSMLIKVLGAPGNCIQRTYLGDHIQGIRLVPIFIGCPPGKRLAFDVSYTIK
  HSEEINKHYFDCVIKDAEMPCFLFRDLFQPFFLVQDLVTGDSGSFLGSYVLKVVGGGRTLNTIRDYTEEE
  IFRYNSPLDTTNSLIWKTKVERTTEDKKFYIMSHESPGVEWLCLENSPCYDIIPQSIYPPEFFFKLLVSN
  RGVDNSTYCDYKLTFIVHIHGLPLSSKRSSFIVMVSTSFFIALVVFYILFCLVWPHIVKAWVSFRWKIHN
  MMAPETYSSSSSSGGFTLHSHSSEGSFEGPSRPGTKEDNVQAKRAKVA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164658
• ORF Size: 3294 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164658.1, NP_001158130.1
• RefSeq Size: 3566 bp
• RefSeq ORF: 3297 bp
• Locus ID: 320225
• UniProt ID: E9Q355
• Cytogenetics: 7 B1