Xrn1 (NM_011916) Mouse Tagged ORF Clone

CAT#: MG221074

Xrn1 (tGFP-tagged) - Mouse 5'-3' exoribonuclease 1 (Xrn1), (10ug)

Product Images

Circular map for MG221074

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xrn1
• Synonyms: Dhm2; exo; mXrn1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221074 representing NM_011916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGTCCCCAAGTTTTACCGATGGATCTCGGAGCGGTACCCCTGCCTCAGCGAAGTGGTGAAGGAGC
  ATCAGATTCCAGAATTTGATAACTTGTACCTGGATATGAATGGGATTATACATCAGTGTTCCCATCCTAA
  TGATGATGATGTTCACTTCAGAATTTCAGATGACAAAATCTTTACTGACATTTTTCACTACTTGGAGGTG
  TTGTTTCGAATTATTAAACCTAGGAAAGTGTTCTTTATGGCTGTTGACGGTGTGGCTCCTCGAGCAAAAA
  TGAACCAGCAACGCGGGAGGCGTTTTAGGTCAGCCAAGGAGGCAGAAGACAAAATTAAAAAAGCAATAGA
  AAAGGGAGAAACACTTCCTACAGAAGCCAGATTCGATTCCAACTGTATTACGCCAGGGACTGAATTCATG
  GCCAGATTGCATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAATCATGGCAAGGTG
  TTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTTATCAGATC
  CGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACATTGTCTTTATGGCTTAGATGCTGACTTGATC
  ATGCTTGGATTAACAAGTCACGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGGTTTGGTGGAAAAA
  AAACACAAAGGGTATGTGCACCAGAAGAAACGACATTTCACCTCCTACACTTGTCTTTAATGAGAGAGTA
  TATTGATTATGAATTTTCAGCATTAAAGGAAAAGATCACATTTAAATATGATATTGAAAAAATTATAGAT
  GATTGGATTTTGATGGGATTTCTTGTTGGCAATGATTTTATCCCTCATCTACCTCATTTACATATTAATC
  ATGATGCGCTGCCTCTTCTTTATGGAACATATATTGCTATCCTGCCAGAACTTGGAGGTTATATTAATGA
  AAGTGGACATCTCAACTTACCTCGATTTGAGAGATACCTTGTGAAACTATCAGATTTTGATCGAGAACAC
  TTCAGTGAAGTTTTTGTGGACCTCAAGTGGTTTGAAAGCAAAGTTGGTAACAAGTATCTCAATGAGGCAG
  CAGGTGCAGCAGCAGAAGAGGCAAAGAACTGCAAGGAGAAGAGAAAACCGAAGGGTCAGGAAAATTCTTT
  AAGTTGGGCTGCATTAGACAAAAGTGAAGGTGAAGGAGTAGCTTCTAGAGATAATTTTGAGGATGAGACT
  GAAGATGATGATCTGTTTGAAACTGAATTTAGACAATATAAGAGAACATACTACATGACGAAGATGGGCG
  TCGATGTAGTATCTGATGAGTTTCTGGCTAATCAGGCTGCCTGTTATGTTCAGGCTATACAGTGGATCTT
  ACACTATTACTATCATGGAGTCCAGTCCTGGAGCTGGTACTATCCCTATCATTACGCACCCTTCCTATCT
  GATATCCGCAGTATCAGTACACTCAAAATCCATTTTGAACTCGGAAAACCCTTCAAGCCATTCGAGCAGC
  TGCTTGCTGTACTTCCATCGGCTAGTAAAAATTTGCTTCCTACATGCTACCAGCATTTGATGACCAGTGA
  AGACTCGCCAATTATAGAATATTATCCACCTGATTTCAAAACTGATCTAAATGGGAAACAACAGGAATGG
  GAAGCTGTGGTATTAATACCGTTTATTGATGAGACGCGATTATTGGAAGCCATGGAGACATGTAACCACT
  CCCTCAAAAAAGAAGAGAGGAAAAGAAACCAGCACAGTGAGTGCCTGATGTGCTGGTATGACAGAGACAC
  AGAGTTCACCTACTCCTCTCCATGGCCAGAAAAGTTCCCTGCCATAGAACGATGTTGTACAAGGTATAAA
  ATGATATCATTAGATGCTTGGCGCGTAGACATAAACAAGAACAAAATAACCAGGGTTGACCAGAAGGCAT
  TATATTTCTGTGGATTCCCTACTTTGAAACACATCAAACATAAATTTTTTTTGAAGAAAAGTGGTGTTCA
  AGTATTCCAGCAGAGTAGTCGTGGTGAAAACTTGATGTTGGAAATTTCAGTAAATGCAGAACCAGATGAA
  CTTAGGATAGAGAACATAGCTTCGGCGGTGCTGGGAAAGGCAGTCTTTGTTAACTGGCCTCATCTTGAAG
  AAGCTAGAGTTGTGGCAGTATCAGATGGTGAAACTAAGTTTTACATAGAAGAACCTCCAGGAACACAGAA
  GGTTTATTTGGGAAAAACTGCCCCACCATCTAAAGTCATCCAACTTACAGATAAAGAACAATCGAACTGG
  ACTAAAGAAATACAAGGAATTTCAGAACAATATCTAAGAAGAAAGGGAATAATAATAAATGAAACATCTG
  CTGTTGTGTATGCTCAGTTGCTCACGGGTCGTAAATATCAAATCAGTCAGAATGGTGAAGTTCGTCTAGA
  AAAACAGTGGTCAAAACAAATTCTTCCTTTTGTTTATCAAACTATTGTTAAGGACATCCGAGCTTTTGAC
  TCTCGTTTCTCCAATATCAAGACATTGGATGACTTGTTTCCACCGAGAACTATGGTCTTTATGCTTGGAA
  CTCCTTATTATGGCTGCACTGGAGAAGTTCAGGATTCAGGAGACTTGATTACAGAAGGGAGAATTCGTGT
  GGTTTTCAGCATTCCATGTGAGCCAAATCTTGATGCTTTAATACAGAATCAGCATAAATACTCTATAAAG
  TACAACCCAGGATATGTGTTGGCCGGTCGCCTTGGCGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAA
  GTATTTTTATTGGAAGAGGATCTAGGAGAAACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCT
  CAAATTCAACAAGAAAAATGAGGAAGTCCCTGGCTATACAAAGAAAGTGGGGAACGAGTGGATGTACTCG
  TCGGCAGCAGAGCAGCTCCTCGCAGAGTACATAGAGAGAGCACCAGAACTGTTCAGTTATATAGCAAAAA
  ACAGCCAAGAAGATGTATTCTATGAAGATGACATCTGGCCTGGAGAAAATGAGAACGGGGCTGAGAAAGT
  TCAAGAAATTATTACTTGGCTAAAAGGACATCCTGTCAGTACTCTGTCTCGGTCTTCTTGTGACTTGCAC
  ATTCTGGATGCAGCCATTGTTGAAAAAATCGAGGAAGAAGTTGAGAAGTGCAAGCAAAGAAAGAGCAACA
  AGAAAGTCCGAGTAACAGTGAAGCCCCATTTGCTGTACAGACCCTTAGAGCAGCAGCATGGAGTCATTCC
  TGATCGGGATGCCGAGTTCCGGCTCTTTGATCGCGTTGTGAACGTGAGAGAGAGCTTCTCCGTGCCAGTC
  GGCCTTCGAGGCACCGTCATTGGAATTAAAGGGGCTAGCAGAGAAGCTGATGTACTATTTGAAGTATTAT
  TTGATGAGGAATTTCCCGGAGGCTTAACAATAAGGTGCTCACCTGGTAGGGGATACCGACTGCCAACAAG
  TGCCTTGGTGAACCTTTCTCATGGGAGTCGGTGTGAAACTGGAAACCAGAAGTTGACAGCCATTGTGAAA
  CCACAACCTTCTGTGAGTCACTGTAGTGCAGCTCCCTCCGGGCACTTGGGAGGCCTCAACCACTCTCCTC
  AGTCACCTTTTCTTCCTACTCAGGTACCTACAAAAGGTGATGATGAATTTTGCAACATTTGGCAATCTCT
  ACAGGGAGCTGGAAAAATTCAACACTTGCAGCCGACAGTACAAGAGAAGGGTGCAGTTCTACCTCAAGAA
  ATAAGTCAAGTAACTGAAGGTCATAAATCTGGCTTTACTGACCACAGTGTCAGACATCAGCAGAGAAAAC
  ATGACTCGCAACGAAAATTTAAGGAAGAATATAAAAGTCCTAAAGCTGAGTGTCAGAGTCAAAAGTTGTC
  CAGTAAACAGACTTCTGGAGGGTCTGCCAGATGTAGTATTAAACTATTGAAGAGAAATGAAAGTCCAGGA
  ACTTCAGAAGCCCAGAAGGTTGTAACTAGTTACCCAAATGCAGTGCATAAGCCTCCCTCTGGGATTGAGA
  ACTTTTTAGCATCTTTGAATCTCTCTAAGGAGAATGAAGCACAGCTACCTCATCATGGAGAGCCTCCAGA
  TGAGGCGGATTTGTCCCCGCAGTCCTTTGCTATGAAAGGAACACGGATGCTTAAAGAAATTCTGAAAATT
  GATAGCCCTGACACAAGAGACAGCAAGAATGATATGAAGAAATCTGATAATGAAGCCACTGTTTCTTCTA
  GAAGAGATGAGCGTGGAGTGTCAGCACATCCTAAGCCAAGTAAGAAGCTGACATGCCATATGAACAAGCC
  TCATGGGACTAACGAGTTCCAAAATGTGGCATCGGTGGACAGTGTGTGTTGGCCAGGCCAGATGCCTCCT
  GTGTCCACACCAGTAACTGAACTTTCTCGAATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTCTCCT
  TTCTGCGAACAACACAGACAATGACAGTTTGTCAGGTAAAATTGTCTAATGGCTTACTGGTACATGGGCC
  ACAGTGCCACTCTGAAAGTGAAGCCAAGGAGAGAGCTGCTCTTTTTGCTTTACAGCAACTGGGCTCCTTG
  GGTGTGAGTTTTCCTTTGCCTCCACCCATATTTACAAATTATCCTCCTGCGGTACCACCTGGAGCCGTCC
  CTCCTGTTTTTACCCAACCTACTGCTAATATAATGCCTTCATCGTCTCATCTCTTTGGCTCAGTGTCATG
  GAGACCACCAGTGCCAGTTGCTGGGAATGCCTTCCACTATCCTTCATATCCTGGAACCATGCCCTTGGCT
  GGAGGAGTGCCAGGCGGAGTGCACAGTCAGTTCATACCTCTGCAGGTTACCAAAAAAAGAGTTGCAAACA
  GAAAGAATTTTGAGAATAAGGAGGCCCAGAGTTCTCAGGCCACACCACTTCAGACTAACAAGCCAGGGTC
  TTCTGAAGCCACCAAAATGACTCCACAAGAAAGTCCACCAGCCTCTTCATCATCCTCTCAGGCTGCCCAG
  CCCGTGTCTTCTCATGTTGAAACTGCCTCCCAAGGCCATGTTGGGTCCCAACCAAGGTCAGCGCCAAGCT
  CCTCAAAAAGGAAGTCGAGAAAACTGGCCGTCAACTTCAGCGTTTCTAAACCTTCGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221074 representing NM_011916
  Red=Cloning site Green=Tags(s)
  
  MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEV
  LFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFM
  ARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLI
  MLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSALKEKITFKYDIEKIID
  DWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYIAILPELGGYINESGHLNLPRFERYLVKLSDFDREH
  FSEVFVDLKWFESKVGNKYLNEAAGAAAEEAKNCKEKRKPKGQENSLSWAALDKSEGEGVASRDNFEDET
  EDDDLFETEFRQYKRTYYMTKMGVDVVSDEFLANQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLS
  DIRSISTLKIHFELGKPFKPFEQLLAVLPSASKNLLPTCYQHLMTSEDSPIIEYYPPDFKTDLNGKQQEW
  EAVVLIPFIDETRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFTYSSPWPEKFPAIERCCTRYK
  MISLDAWRVDINKNKITRVDQKALYFCGFPTLKHIKHKFFLKKSGVQVFQQSSRGENLMLEISVNAEPDE
  LRIENIASAVLGKAVFVNWPHLEEARVVAVSDGETKFYIEEPPGTQKVYLGKTAPPSKVIQLTDKEQSNW
  TKEIQGISEQYLRRKGIIINETSAVVYAQLLTGRKYQISQNGEVRLEKQWSKQILPFVYQTIVKDIRAFD
  SRFSNIKTLDDLFPPRTMVFMLGTPYYGCTGEVQDSGDLITEGRIRVVFSIPCEPNLDALIQNQHKYSIK
  YNPGYVLAGRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGNEWMYS
  SAAEQLLAEYIERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLH
  ILDAAIVEKIEEEVEKCKQRKSNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFRLFDRVVNVRESFSVPV
  GLRGTVIGIKGASREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRCETGNQKLTAIVK
  PQPSVSHCSAAPSGHLGGLNHSPQSPFLPTQVPTKGDDEFCNIWQSLQGAGKIQHLQPTVQEKGAVLPQE
  ISQVTEGHKSGFTDHSVRHQQRKHDSQRKFKEEYKSPKAECQSQKLSSKQTSGGSARCSIKLLKRNESPG
  TSEAQKVVTSYPNAVHKPPSGIENFLASLNLSKENEAQLPHHGEPPDEADLSPQSFAMKGTRMLKEILKI
  DSPDTRDSKNDMKKSDNEATVSSRRDERGVSAHPKPSKKLTCHMNKPHGTNEFQNVASVDSVCWPGQMPP
  VSTPVTELSRICSLVGMPQPDFSFLRTTQTMTVCQVKLSNGLLVHGPQCHSESEAKERAALFALQQLGSL
  GVSFPLPPPIFTNYPPAVPPGAVPPVFTQPTANIMPSSSHLFGSVSWRPPVPVAGNAFHYPSYPGTMPLA
  GGVPGGVHSQFIPLQVTKKRVANRKNFENKEAQSSQATPLQTNKPGSSEATKMTPQESPPASSSSSQAAQ
  PVSSHVETASQGHVGSQPRSAPSSSKRKSRKLAVNFSVSKPSE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011916
• ORF Size: 5169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011916.3, NP_036046.2
• RefSeq Size: 5515 bp
• RefSeq ORF: 5172 bp
• Locus ID: 24127
• Cytogenetics: 9 E3.3