Tacc2 (NM_206856) Mouse Tagged ORF Clone

CAT#: MG221067

Tacc2 (tGFP-tagged) - Mouse transforming acidic coiled-coil containing protein 2 (Tacc2) transcript variant 1, (10ug)

Product Images

Circular map for MG221067

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tacc2
• Synonyms: mKIAA4180
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221067 representing NM_206856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACGAGAACAGCACCTCGGACCACCAGAGGACTTCTTCAGTTCAGAGTCCAAGGTCACTGCAGC
  CACCTGGGAAGAGTCAATCTCTACAGAAGCAACAAGGAGACTTGCCTGGATCGTGTGCTGGAAGTATCCC
  TGGAACTGATGATGTCATCCAGCCAGCTGCTCCTGTTGACCCGGGACACCCACCCTTAGCTGACTCGTCC
  CACCATGGTGACGCTGTCAGCTCAGTCTCTACACATCTGACAGTCCAGAGCGCCTCCCCATCTGCTGCCC
  GTGCAAGTCCTGCTCCTTTGGCCCCAGAACATACAGCCTCGGCTCCCTCCGCAGCCGGTCCTGGAGTAGA
  AGTCACCCCCACTGCCTCCCCCCAACACCTGGCCAAGAACGAACCCAGGAGTTCCGACTCTGAGGAAGCC
  TTTGAGACCCCCGAGTCAACGACCCCTGTCAAAGCTCCACCAGCCCCTCCCCCTCCACCCCCTGAAGTCA
  CCCCAGAGCCTGAGGTCATCGATCCACCAGCCCCAGAAGAACCAGGATGCATTTCTGAGCCACCGGTTGT
  GGTTCCCGATGGCCCTCGCAGCAGCGAGTCCGTGGAAGGAAGCCCTTTCCGTCCTTCCCACTCCTCCTCG
  GCGGTGTTCGATGAAGACAAGCCGATAGCCAGCAGCGGGACTTACAACTTAGACTTCGACAGCATCGAGC
  TGGTGGATAACTTCCAGAGCTTGGAGCCGTGCTCTGCCGACTCTAAGGGTCAGGAGTGTAAGGTGAGCAC
  GCGGAGGAAATCCACCGAGTCCGTGCCACCCTCCAAGTCCACGCTGTCCCGTTCGCTTAGCCTACAAGCC
  AGTGACTTTGACGGTGCTTCTTGCCCCGGCAGCCCTGAAGCTGGGACCCTTACCACAGATGCATGTGGCA
  CAGGATCCAACAGTGCTTCCAGCACCCTTAAGCGAACTAAAAAAACACGGCCACCTTCCTTGAAAAAGAA
  GCAAGCCACCAAGAAACCCACAGAAACCCCCCCAGTGAAGGAGACCCAACAGGAACCAGGTGAAGAGAGT
  CCAGTCCCCAGTGAGGAACACTTAGCACCAGAGACAAAGACGGAGTCGGCCACACCTGAGGGCGCTGGTT
  GCACCTTGTCAGACGATACACCCCTGGAGTCCCCTGCTGTCCCCACAGCCACCTGTCCTCTGACTTTGGA
  GAGTGCAGAAGACGTTAGCCCCCTGGTTTCTGGAGGTGGCAGAGTGCAGAACTCACCCCCAGTTGGGAGG
  AAATCAGTGCCTCTTACCACAGCCTCTGAGGCGGTGGAGGTGACCCTGTCGGACAGTGGGGGGCAAGAGG
  ACTTGCCTGCCAAGGGGTTGTCAGTGAGGCTGGAGTTTGACTATTCTGAGGACAAGGGAAGTTGGGAGAG
  TCAGCAGGAAAACGCCCCTCCCACCAAAAAGATAGGCAAGAAGCCAGTTGCCAAAATGCCCCTAAGGAGG
  CCAAAGATGAAAAAGACCCCCGAGAAACTTGACAACACTCCTGCCTCACCTCCAAGGTCCCCTACTGAAC
  CCAGTGACACCCCTATTGCTAAAGGTACCTACACCTTTGATATAGACAAGTGGGATGACCCCAATTTTAA
  CCCTTTTTCCTCCACCTCAAAAATGCAAGAGTCTCCCAAACTGTCCCAACAATCGTACAACTTTGACCCT
  GACGCCTGTGAAGAGTCCCTTGACCCCTTTAAGGCATCCTCTAAGACCCCCAGCTCACCTTCTAAGTCCC
  CAGCCTCTTTTGAGATCCCAGCCAGCACCACGGAAGCGGATGGGGATGGGTTGAATAAACCTGCCAAGAA
  GAAGAAGACTCCACTAAAGACGATGGTTGAAGATGTGATGTCTGTGTGTTCTCTGTTTGACACGTTTAGG
  GTGAAGAAGTCTCCAAAGCGGTCTCCTCTGTCTGACCCGCCTTCTCAGGACCCCACTCCAGCTGCCACGC
  CGGAAGCACCCTCAGCCATCTCTACAGTGGTCCACGCCACAGATGAGGAGAAGCTGGCAGTCACTAGCCA
  GAAGTGGACGTGTATGACGGTGGACTTGGATGCTGACAAACAGGACTTCCCTCAGCCCTCGGACCTGTCT
  AACTTTGTAAATGAGACCAAATTCAATTCACCCTCAGAGGAGCTGGACTACAGAAACTCCTATGAAATTG
  AATACATGGAAAAGCTTGGCTCCTCCTTACCTCAGGACGATGACACTCCGAAGAAGCAGGCCTTGTACCT
  TATGTTTGACACTCCTCAGGAGAGCCCCGTCAAGTCTCCTCCAGTCCGGATGTCAGATTCCCCAACTCCA
  TGTTCAGGGTCGAGTTTTGAGGACACCGAAGCCCTTGTGAATGCAGCAACAAAGCTCCAGCATCCAGTCG
  CAAGAGGTCTGCCCTCCAGTCAGGAGCCTCTCTTGCAGGTGCCTGAGAAGCCCTCCCAGAAGGAGCTGGA
  GGCCATGGCCTTGGGCACCCCAGCAGAAGCAATTGAAATCACAGCTCCCGAGGGTGCCTTTGCTTCTGCA
  GATACCCTCCTCAGCAGGCTGGCCCATCCCGCCTCTCTCTGTGGTGCACTTGGCTATCTGGAGCCTGACT
  TAGCAGAAAAGAACCCCCCAGTATTTGCCCAGAAACTTCAGGAGGAGTTAGAGTTTGCTGTCATGCGGAT
  AGAAGCCCTGAAGCTGGCCAGGCAGATCGCCCTGGCTTCCCGCAGCCGCCAGGACACCAAGAGGGAAGCT
  GCTCACCCACCAGATGTCTCCATCTCCAAAACTGCCTTGTACTCCCGCATCGGGTCCACCGAGGTGGAGA
  AACCCCCAGGCCTTCTGTTCCAGCAGCCAGACCTGGACTCTGCACTCCAGGTTGCCAGAGCAGAGGTCAT
  CGCCAAGGAGAGAGAAGTCTCAGAGTGGAGGGATAAATATGAAGAAAGCCGACGGGAAGTGGTGGAAATG
  AGGAAAATTGTGGCCGAATATGAGAAGACCATTGCACAGATGATCGAGGACGAACAGAGAGAAAAATCCA
  TCTCCCACCAAACTGTACAGCAGCTGGTCCTGGAGAAGGAGCAAGCCCTGGCTGACCTGAACTCTGTGGA
  AAAGTCTCTGGCTGACCTCTTCAGGAGATACGAAAAGATGAAGGAGGTCCTGGAAGGTTTTCGAAAGAAT
  GAAGAGGTGTTGAAGAAATGTGCGCAGGAGTACCTATCCCGAGTGAAGAAAGAGGAACAGAGGTACCAGG
  CCCTGAAGGTGCACGCCGAAGAGAAACTGGACAGAGCCAATGCAGAGATTGCCCAGGTTCGCGGCAAGGC
  CCAGCAAGAGCAAGCAGCCTACCAGGCTAGCCTGCGGAAGGAGCAGCTTCGAGTGGATGCTCTGGAAAGA
  ACGCTGGAGCAGAAGAATAAAGAGATAGAAGAACTCACCAAGATTTGTGACGAGCTGATCGCCAAGATGG
  GAAAAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221067 representing NM_206856
  Red=Cloning site Green=Tags(s)
  
  MGNENSTSDHQRTSSVQSPRSLQPPGKSQSLQKQQGDLPGSCAGSIPGTDDVIQPAAPVDPGHPPLADSS
  HHGDAVSSVSTHLTVQSASPSAARASPAPLAPEHTASAPSAAGPGVEVTPTASPQHLAKNEPRSSDSEEA
  FETPESTTPVKAPPAPPPPPPEVTPEPEVIDPPAPEEPGCISEPPVVVPDGPRSSESVEGSPFRPSHSSS
  AVFDEDKPIASSGTYNLDFDSIELVDNFQSLEPCSADSKGQECKVSTRRKSTESVPPSKSTLSRSLSLQA
  SDFDGASCPGSPEAGTLTTDACGTGSNSASSTLKRTKKTRPPSLKKKQATKKPTETPPVKETQQEPGEES
  PVPSEEHLAPETKTESATPEGAGCTLSDDTPLESPAVPTATCPLTLESAEDVSPLVSGGGRVQNSPPVGR
  KSVPLTTASEAVEVTLSDSGGQEDLPAKGLSVRLEFDYSEDKGSWESQQENAPPTKKIGKKPVAKMPLRR
  PKMKKTPEKLDNTPASPPRSPTEPSDTPIAKGTYTFDIDKWDDPNFNPFSSTSKMQESPKLSQQSYNFDP
  DACEESLDPFKASSKTPSSPSKSPASFEIPASTTEADGDGLNKPAKKKKTPLKTMVEDVMSVCSLFDTFR
  VKKSPKRSPLSDPPSQDPTPAATPEAPSAISTVVHATDEEKLAVTSQKWTCMTVDLDADKQDFPQPSDLS
  NFVNETKFNSPSEELDYRNSYEIEYMEKLGSSLPQDDDTPKKQALYLMFDTPQESPVKSPPVRMSDSPTP
  CSGSSFEDTEALVNAATKLQHPVARGLPSSQEPLLQVPEKPSQKELEAMALGTPAEAIEITAPEGAFASA
  DTLLSRLAHPASLCGALGYLEPDLAEKNPPVFAQKLQEELEFAVMRIEALKLARQIALASRSRQDTKREA
  AHPPDVSISKTALYSRIGSTEVEKPPGLLFQQPDLDSALQVARAEVIAKEREVSEWRDKYEESRREVVEM
  RKIVAEYEKTIAQMIEDEQREKSISHQTVQQLVLEKEQALADLNSVEKSLADLFRRYEKMKEVLEGFRKN
  EEVLKKCAQEYLSRVKKEEQRYQALKVHAEEKLDRANAEIAQVRGKAQQEQAAYQASLRKEQLRVDALER
  TLEQKNKEIEELTKICDELIAKMGKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206856
• ORF Size: 3438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206856.4
• RefSeq Size: 3906 bp
• RefSeq ORF: 3441 bp
• Locus ID: 57752
• UniProt ID: Q9JJG0
• Cytogenetics: 7 F3
• Gene Summary: This gene encodes a member of the transforming, acidic coiled-coil (TACC) family of proteins. Members of this family are centrosomal proteins that interact with microtubules and tubulin. TACC proteins are thought to be involved in centrosome/mitotic spindle dynamics and gene regulation. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]