Xirp1 (NM_011724) Mouse Tagged ORF Clone

CAT#: MG220984

Xirp1 (tGFP-tagged) - Mouse xin actin-binding repeat containing 1 (Xirp1), (10ug)

Product Images

Circular map for MG220984

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xirp1
• Synonyms: AI415219; Cmya1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220984 representing NM_011724
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGCCCAGATGCAGGTAGCTCCTACACCAACCATCCAAATGAGGACAGAAGAGGACCTGTCCC
  TCCCTCATCCCTCAGCCCCAGAGGGTCTGCCGCCACCACCCCCCAAAGAAACCTTCTCCAAGTTCCAGCA
  GCAGCGGCAAGCCAGTGAGCTCCGGCGTCTCTACAAACACATCCACCCTGAGCTTCGGAAGAACCTCGAG
  GAGGCTGTGGCTGAGGACCTGGCGGAGGTCCTCGGTTCGGAGGAGCCCACTGAGGGCGATGTTCAGTGTA
  TGCGCTGGATCTTTGAGAACTGGCGGCTAGATGCCATTGGCGATCACGAGAGGCCGGCTGCCAGGGAACC
  TGTGTCAGGTGGCAACGTCCAGGCCACCTCTCGAAAGTTTGAGGAAGGCTCCTTTACCAACAGCTCAGAT
  CAGGAGCCGGAGGGACTCCGGCCATCAGGGGGTGATGTCCAAGCTGCCAGACAGATGTTTGAGACCAAGC
  CACTGGATGCGCTGAGAGGCCAGGAGGAGGCAACACAGACAACCATGAGGGAACCTGCAGCCACTGGAGA
  TGTACAGGGAACCAGAAAGCTCTTTGAGACTAGGCCCCTGGACCGACTGGGCTCCCGCCCCTCTATCCAG
  GAGCAGAGTCCTTTAGAGCTGCGCTCAGAGATTCAGGAGCTGAAGGGCGATGTGAAGAAGACGGTGAAGC
  TGTTTCAGACGGAACCTCTATGCGCCATCCAGGATGCCGAGGGCACCATCCACGAAGTCAAGGCTGCCTG
  TCGGGAGGAGATTCAAAGCAATGCAGTGAGGTCTGCTCGCTGGCTCTTTGAGACCCGACCTCTGGATGCC
  TTCAACCAGGACCCCAGCCAGGTGCGGGTGATTCGGGGGATCTCCCTTGAGGAGGGAGCCCTGCCGGATG
  TCAGTGCAACTCGTTGGATCTTTGAGACGCAGCCTTTGGATGCCATTCGTGAGATCGAGGTGGACGAGAA
  GGACTTCCAGCCATCTCCGGATCTCATCCCTCCTGGCCCAGATGTTCAGCATCAGCGACATCTGTTTGAA
  ACCTGTTCCCTGGACACTCTGAAGGGTGAAAGAGAAACAGAGGCAGAGGTGCCACCCAAGGAGGAGGTGA
  TTCCTGGCGATGTCCGCTCTACACTCTGGCTATTTGAAACAAAACCCCTGGATGCTTTCAGAGACCAGGT
  CCAAGTGGGTCACCTGCAACGGGTGGGTCACCAGGAGGGTGAGGGGCTTGTGACAGAGTGTCTACCCAGT
  AATGGTACTTCGGTGTTGCCTCTGTCTCAGGGTGTCCCCCAGAATGATGGGTTGAAAGGGGATGTGAAAA
  CCTTCAAGAATCTTTTTGAGACCCTTCCCTTAGACAGCATTGGGCAGGGTGAGCCTTCAGCCTATGGGAA
  CATAAACAGAGGACAAAACACTGATTCTGCCGAGCAGTCCCAGGGTTCTGACGCCCCGGTATATGCTATG
  CAGGACAGCAGGGGACAGCTTCATGCCCTGACTTCTGTCAGCAGAGAGCAGGTAGTTGGAGGTGATGTTC
  AGGGCTACAAGTGGATGTTTGAGACACAGCCCCTGGACACGCTGGGTAGAAGCCCCAGTACCATCGATGT
  GGTACGAGGTATCACTCGGCAGGAAGTGGTGGCTGGGGATGTCGGCACCACTCGGTGGCTCTTTGAGACG
  CAGCCATTGGAGATGATCCACCAACAAGAGCAACAGAAACCAGAGGAGGAAGAGGGGAAGGGTCCAGGAG
  GCCCCCCCCCTGAGCTCCCCAAAAAGGGTGATGTACAGACTATCCGTTGGCTGTTTGAGACCTACCCTAT
  GAGCGAGTTGGCAGAGAAGCGAGAATCTGAGGTCACAGACCCCGTGAGCAAGGCTGAGACACAGTCCTGC
  ACCTGGATGTTTGGGCCCCAATCTCTGAACCCAGCAGAAGGCTCTGGGGAGCAGCACCTGCAAACCAGCC
  AGGTTCCGGCTGGAGACAGGCAGACAGACAGACATGTCTTTGAGACTGAATCTCTGCCAGCCTCAAACCA
  ATCCAGTGGGAGAAAGCCTGTAAGGTACTGCAGCCGGGTGGAGATTCCTTCCGGGCAGGTATCTCGGCAG
  AAAGAGGTTTTCCAGGCCCTGGAGGCGGGCAAGAAGGAGGTCCCGGAGACCACAATCAACCTGGGGTCCA
  TCCCCACAGGCTCTGTGCATAAATTCACCTGGCTCTTTGAAAACTGTCCCATGGGCTCCCTGGCAGCTGA
  GAGCATCAGAGGGGACAACCTCCAGGAAGAACAGCCAAAAGGCTCTGCAGGCCATGGGACACCAGAGAGG
  CAGGAGACAGCAGCCGAGAGAACCCTTCGGACTTTGCATGCCACCCCCGGCATCCTGCACCATGGAGGCA
  TCCTCATGGAGGCCCGAGGGCCAGGGGAGCTCTGTCTTGCCAAGTATGTGCTCCCAAGCCCAGGGCAGGG
  GCGCCCCTACATACGGAAGGAGGAGCTGGTGTGTGGTGAGCTTCCCAGGATCGTCCGTCAAGTGCTGCGC
  CGGACAGACGTGGACCAGCAGGGACTGCTGGTTCAGGAGGACACAGCTGGGCAGCTCCAGCTCCACCCAC
  TCACGCTGCCAGGGCCTGGTGACCCTGGGAATATTGAAGACATGGACCCTGAGCTCCAGCAGCTGCTGGC
  CTGTGGCCTGGGAGTCTCTGTGTCAAAGACGGGGCTGGTGATGCAAGAGACAGGACAGGGCCTAGTGGCA
  CTGACTGCCTACTCCCTCCAGCCCCAGCTTACCAGCAGGGCCCCTGAAAGAAGCAGTGTGCAGCTGCTGG
  CCAGCTGCATAGACAAAGGAGACCTGCACAGCCTTCACAGTTTGCGGTGGGAGCCGCCAACAGACCCAAG
  TTCTGGACCTGCTACTGAGGAGTCCCAGAGGGTCCCCCAAACTGAGAGCATCATACATGTTACCCCATTA
  GACTCCACCATGGAGATGGGGCAGCTGAGAATCTCAGGATCCACACCCTGCCCACCTCCATCTCGGGCAG
  CTGGAAAAGTGGTCCTTCCAAATGGGAAACCTGTAGCCCAAGCTCCATTGCAGGAAGCAAGGAAGAAAAC
  GGATATCAGCCATGCTGGGCAGAAAGGGAAGGCAGCCTCAGGAAGACCAGAAGGAACCATTGCTTCCCCT
  CTAGGGTCTGGGGCTCCAGATCTCCAGGAAGCCATGCAGAATCTGCGTTTGGCAACCGCTGAGGCCCAAA
  GCCTGCACCAGCAGGTTCTGAGCAGGCATCCACAGGGCTCTGACCCTGTAGCCACCTCCATGCCTGTCCA
  AGATGTTCTGCAAGCATCCACTCCTGCCACTGGGGTTACTCAAGGCAGCATCAGGCCTGTGGCTGGAAGT
  GAAGCCAGGATCCCAGCATTCCCCAGAAAGCTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220984 representing NM_011724
  Red=Cloning site Green=Tags(s)
  
  MADAQMQVAPTPTIQMRTEEDLSLPHPSAPEGLPPPPPKETFSKFQQQRQASELRRLYKHIHPELRKNLE
  EAVAEDLAEVLGSEEPTEGDVQCMRWIFENWRLDAIGDHERPAAREPVSGGNVQATSRKFEEGSFTNSSD
  QEPEGLRPSGGDVQAARQMFETKPLDALRGQEEATQTTMREPAATGDVQGTRKLFETRPLDRLGSRPSIQ
  EQSPLELRSEIQELKGDVKKTVKLFQTEPLCAIQDAEGTIHEVKAACREEIQSNAVRSARWLFETRPLDA
  FNQDPSQVRVIRGISLEEGALPDVSATRWIFETQPLDAIREIEVDEKDFQPSPDLIPPGPDVQHQRHLFE
  TCSLDTLKGERETEAEVPPKEEVIPGDVRSTLWLFETKPLDAFRDQVQVGHLQRVGHQEGEGLVTECLPS
  NGTSVLPLSQGVPQNDGLKGDVKTFKNLFETLPLDSIGQGEPSAYGNINRGQNTDSAEQSQGSDAPVYAM
  QDSRGQLHALTSVSREQVVGGDVQGYKWMFETQPLDTLGRSPSTIDVVRGITRQEVVAGDVGTTRWLFET
  QPLEMIHQQEQQKPEEEEGKGPGGPPPELPKKGDVQTIRWLFETYPMSELAEKRESEVTDPVSKAETQSC
  TWMFGPQSLNPAEGSGEQHLQTSQVPAGDRQTDRHVFETESLPASNQSSGRKPVRYCSRVEIPSGQVSRQ
  KEVFQALEAGKKEVPETTINLGSIPTGSVHKFTWLFENCPMGSLAAESIRGDNLQEEQPKGSAGHGTPER
  QETAAERTLRTLHATPGILHHGGILMEARGPGELCLAKYVLPSPGQGRPYIRKEELVCGELPRIVRQVLR
  RTDVDQQGLLVQEDTAGQLQLHPLTLPGPGDPGNIEDMDPELQQLLACGLGVSVSKTGLVMQETGQGLVA
  LTAYSLQPQLTSRAPERSSVQLLASCIDKGDLHSLHSLRWEPPTDPSSGPATEESQRVPQTESIIHVTPL
  DSTMEMGQLRISGSTPCPPPSRAAGKVVLPNGKPVAQAPLQEARKKTDISHAGQKGKAASGRPEGTIASP
  LGSGAPDLQEAMQNLRLATAEAQSLHQQVLSRHPQGSDPVATSMPVQDVLQASTPATGVTQGSIRPVAGS
  EARIPAFPRKLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011724
• ORF Size: 3396 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011724.3, NP_035854.3
• RefSeq Size: 5839 bp
• RefSeq ORF: 3399 bp
• Locus ID: 22437
• Cytogenetics: 9 F4