Slc38a3 (NM_023805) Mouse Tagged ORF Clone

CAT#: MG220834

Slc38a3 (tGFP-tagged) - Mouse solute carrier family 38 member 3 (Slc38a3), (10ug)

Product Images

Circular map for MG220834

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc38a3
• Synonyms: 0610012J02Rik; D9Ucla2; mNAT; Nat1; Slc38-3; Sn1; Snat3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220834 representing NM_023805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATACCCCGACAGACAGAGATGGTGGAGCTGGTGCCCAATGGCAAACACTTGGAGGGGCTTCTAC
  CAGTGGGCGTGCCTACAACAGACACCCAGAGGACTGAAGACACCCAACACTGTGGAGAGGGCAAGGGCTT
  CCTTCAGAAGAGTCCCAGCAAGGAGCCACACTTCACCGATTTCGAGGGGAAGACATCATTTGGGATGTCA
  GTGTTCAATCTCAGCAACGCCATCATGGGCAGTGGAATTCTGGGGCTCGCCTACGCCATGGCCAATACGG
  GCATCATCCTTTTCCTGTTCCTGCTTACAGCGGTCGCCCTGTTGTCTAGCTATTCCATCCACCTGCTCCT
  CAAGTCTTCGGGGATTGTGGGCATCCGTGCCTACGAGCAGTTGGGCTACCGTGCCTTTGGGACCCCGGGG
  AAGCTGGCAGCAGCCCTGGCCATTACGCTTCAGAACATTGGAGCCATGTCCAGCTACCTATACATCATCA
  AGTCTGAATTGCCTCTTGTCATACAGACCTTCCTGAATCTGGAGAAGCCGGCCTCGGTGTGGTACATGGA
  TGGCAACTACCTTGTGATCCTGGTTTCTGTCACCATCATTCTGCCCCTAGCACTGATGCGACAGCTCGGC
  TACCTGGGTTACTCCAGTGGCTTCTCTCTCAGCTGCATGGTGTTCTTCTTGATCGCAGTCATCTATAAGA
  AGTTCCAAGTTCCTTGCCCATTGGCACACAACCTGGCCAATGCCACCGGCAACTTCAGCCACATGGTGGT
  GGCAGAGGAGAAGGCACAGCTGCAGGGCGAGCCTGACACTGCTGCTGAGGCCTTCTGTACCCCAAGCTAC
  TTCACCCTCAACTCACAGACAGCATACACCATCCCCATCATGGCTTTCGCCTTCGTCTGCCACCCTGAGG
  TGCTGCCCATATATACAGAGCTCAAGGACCCCTCCAAGAGGAAGATGCAGCACATCTCCAACCTGTCCAT
  TGCTGTCATGTATGTCATGTACTTCCTGGCCGCCCTCTTCGGCTACCTCACCTTCTACGACGGGGTGGAG
  TCGGAGCTGCTGCACACCTACAGCAAGGTGGACCCGTTTGATGTGCTGATCTTGTGTGTGCGAGTGGCCG
  TGCTGATAGCGGTCACACTTACAGTTCCGATTGTTCTGTTCCCGGTACGACGTGCTATCCAGCAGATGCT
  GTTTCAGAACCAGGAGTTCAGCTGGTTGCGGCACGTGCTCATTGCCACTGGCCTGCTTACGTGCATCAAT
  CTGCTGGTTATCTTCGCCCCCAACATCTTGGGCATATTTGGGATCATTGGTGCCACATCTGCTCCGTGCC
  TCATCTTCATCTTCCCTGCCATCTTCTACTTCCGAATCATGCCCACTGACAAGGAGCCTGCAAGATCCAC
  CCCTAAAATCCTGGCCCTTTGTTTCGCTGCGGTTGGCTTCCTGCTGATGACCATGAGCCTGAGTTTCATC
  ATCATTGACTGGGTCTCTGGGACCAGCCAGCATGGAGGAAACCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220834 representing NM_023805
  Red=Cloning site Green=Tags(s)
  
  MEIPRQTEMVELVPNGKHLEGLLPVGVPTTDTQRTEDTQHCGEGKGFLQKSPSKEPHFTDFEGKTSFGMS
  VFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHLLLKSSGIVGIRAYEQLGYRAFGTPG
  KLAAALAITLQNIGAMSSYLYIIKSELPLVIQTFLNLEKPASVWYMDGNYLVILVSVTIILPLALMRQLG
  YLGYSSGFSLSCMVFFLIAVIYKKFQVPCPLAHNLANATGNFSHMVVAEEKAQLQGEPDTAAEAFCTPSY
  FTLNSQTAYTIPIMAFAFVCHPEVLPIYTELKDPSKRKMQHISNLSIAVMYVMYFLAALFGYLTFYDGVE
  SELLHTYSKVDPFDVLILCVRVAVLIAVTLTVPIVLFPVRRAIQQMLFQNQEFSWLRHVLIATGLLTCIN
  LLVIFAPNILGIFGIIGATSAPCLIFIFPAIFYFRIMPTDKEPARSTPKILALCFAAVGFLLMTMSLSFI
  IIDWVSGTSQHGGNH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023805
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023805.3, NP_076294.2
• RefSeq Size: 2622 bp
• RefSeq ORF: 1518 bp
• Locus ID: 76257
• UniProt ID: Q9DCP2
• Cytogenetics: 9 58.69 cM
• Gene Summary: Sodium-dependent amino acid/proton antiporter. Mediates electrogenic cotransport of glutamine and sodium ions in exchange for protons. Also recognizes histidine, asparagine and alanine. May mediate amino acid transport in either direction under physiological conditions. May play a role in nitrogen metabolism and synaptic transmission.[UniProtKB/Swiss-Prot Function]