Dlgap4 (NM_001042487) Mouse Tagged ORF Clone

CAT#: MG220808

Dlgap4 (tGFP-tagged) - Mouse discs large homolog-associated protein 4 (Drosophila) (Dlgap4) transcript variant 2, (10ug)

Product Images

Circular map for MG220808

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dlgap4
• Synonyms: AI225853; BC024558; DA; DAP-4; DAP4; S; SAPAP-4; Sapap4; WBP1; WBP16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220808 representing NM_001042487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGTGTCTGGAGCTCTTGAAGCAATGTTCATCATGCCTAGTGGCATATAAGAAGACCCCACCAC
  CGGTCCCTCCACGAACCACATCGAAACCGTTCATCTCAGTCACCGTCCAGAGCAGTACTGAGTCTGCTCA
  GGATACCTACCTGGACAGTCAAGACCACAAGAGCGAAGTGACGAGCCAGTCGGGCTTGAGCAACTCATCA
  GACAGCCTGGACAGCAGTACCCGGCCACCCAGTGTGACACGGGGTGGAATTACCCCAGGCCCTGAAGCTC
  CGGAGCCACCCCCTAAGCATGCAGCTCTGAAGAGTGAACAAGGGACACTGACGAGCTCTGAGTCCCATTC
  CGAGGCCATCCCCAAAAGGAAACTGTCATCGATAGGAATACAAGTTGACTGCATTCAGCCAGTGCCAAAA
  GAGGAGCCCAGTCCCGCTACCAAATTCCAGTCCATCGGGATTCAGGTAGAGGACGACTGGCGAAGCAGTG
  CCCCCTCTCACAGCATGTCCTCCCGTCGGGACACTGACTCTGATACCCAGGATGCCAACGACTCCAGTTG
  TAAGTCATCTGAGAGGAGTCTCCCAGACTGTACCTCACACCCCAATTCCATCAGCATTGATGCTGGCCCC
  CGACAGGCCCCCAAGATTGCCCAGATCAAGCGCAACCTCTCCTATGGAGACAACAGCGACCCTGCCCTGG
  AGGCGTCTTCGCTGCCCCCACCCGACCCTTGGCTGGAGACTTCCTCCAGCTCCCCAGCAGAGCCCGCTCA
  GCCAGGGGCCTGCCGCAGGGATGGCTACTGGTTCCTGAAGCTCCTTCAGGCAGAAACGGAGAGACTGGAA
  GGCTGGTGCTGCCAGATGGACAAGGAGACCAAAGAGAACAACCTCTCTGAAGAAGTCTTAGGGAAAGTTC
  TCAGTGCTGTGGGCAGCGCCCAGCTCCTGATGTCCCAGAAATTCCAGCAGTTCCGCGGCCTCTGTGAGCA
  GAACTTGAACCCTGATGCCAACCCCCGTCCGACAGCCCAGGACCTGGCAGGGTTCTGGGACCTGCTGCAG
  CTGTCCATTGAGGACATCAGCATGAAGTTCGATGAGCTCTACCACCTCAAGGCCAACAGCTGGCAGCTGG
  TGGAGACCCCCGAGAAGAGGAAGGAAGAGAAGAAGCCACCCCCTCCAGTCCCGAAGAAGCCAGCCAAATC
  CAAGGCGGCAGTGAGCCGCGACAAGGCCTCAGACGCGGGGGACAAGCAGCGTCAGGAGGCCAGAAAGAGA
  CTCCTGGCCGCCAAGCGAGCAGCGTCTGTGCGGCAGAACTCGGCCACAGAGAGTGCGGACAGCATCGAGA
  TTTATGTCCCCGAGGCCCAGACCAGGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220808 representing NM_001042487
  Red=Cloning site Green=Tags(s)
  
  MALCLELLKQCSSCLVAYKKTPPPVPPRTTSKPFISVTVQSSTESAQDTYLDSQDHKSEVTSQSGLSNSS
  DSLDSSTRPPSVTRGGITPGPEAPEPPPKHAALKSEQGTLTSSESHSEAIPKRKLSSIGIQVDCIQPVPK
  EEPSPATKFQSIGIQVEDDWRSSAPSHSMSSRRDTDSDTQDANDSSCKSSERSLPDCTSHPNSISIDAGP
  RQAPKIAQIKRNLSYGDNSDPALEASSLPPPDPWLETSSSSPAEPAQPGACRRDGYWFLKLLQAETERLE
  GWCCQMDKETKENNLSEEVLGKVLSAVGSAQLLMSQKFQQFRGLCEQNLNPDANPRPTAQDLAGFWDLLQ
  LSIEDISMKFDELYHLKANSWQLVETPEKRKEEKKPPPPVPKKPAKSKAAVSRDKASDAGDKQRQEARKR
  LLAAKRAASVRQNSATESADSIEIYVPEAQTRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042487
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042487.1, NP_001035952.1
• RefSeq Size: 3631 bp
• RefSeq ORF: 1362 bp
• Locus ID: 228836
• UniProt ID: B1AZP2
• Cytogenetics: 2 H1
• Gene Summary: This gene encodes a membrane-associated guanylate kinase found at the postsynaptic density in neuronal cells. The encoded protein may play a role in synapse organization and neuronal signalling. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Mar 2013]