Cblc (NM_023224) Mouse Tagged ORF Clone

CAT#: MG220739

Cblc (tGFP-tagged) - Mouse Casitas B-lineage lymphoma c (Cblc) transcript variant 1, (10ug)

Product Images

Circular map for MG220739

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cblc
• Synonyms: 2310076I21Rik; 2310079L19Rik; Cbl3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220739 representing NM_023224
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCGGCAGCCCCGCGAGGGTGGCAGCGGGGCGAGCCACGCGCGCTTAGCCGGGCGGTGAAGT
  TGCTCCAGCGCCTAGAAGAGCAATGCAGGGATCCCAGGATGGTCACGGGGCCCCCGTCCCTGCGGGACCT
  GCTGCCCCGCACCGCGCAGCTACTTGGAGAGGTGGCAAAGGCCCGGCGCGAGGCCAGGGAAGACCCCGAG
  GGTCCCGGTGGCGCCGATGACTTTCTGGCCATCTACCTGGCCAATCTGGAGGTCAAAGGCAGGCAGGTGG
  CGGAGCTCCTGCCACCCCGAGGCAAAAAGGACGTGAACCAGGATGTTTTCCGGGAGGGCTCCAGATTCAG
  GCGACAACTGGCCAAGCTGGCCCTCATCTTCAGTCACATGCACGCGGAGTTGAGCGCACTCTTCCCTGCT
  GGGAAGTACTGTGGGCACCTGTACCAGCTCACCAAGGGCTCTGCCCACATCTTCTGGAGGCAGAATTGTG
  GAGTTCGGTGCGTGCTTCCCTGGGCTGAGTTCCAGTCCCTCCTGTGTGCCTGCCACCCTGTGGAACCAGG
  CCCCACCATGCAGGCCTTGCGGTCCACCTTGGACCTCACCTGTAACGGCCACGTGTCTGTCTTTGAGTTT
  GACGTCTTCACCAGGCTCTTTCAGCCGTGGCCCACTTTACTGAGGAATTGGCAACTCCTGGCTGTCAACC
  ATCCTGGCTACATGGCCTTCCTCACCTACGATGAGGTCCAAACACGCCTGCAGGCCTACAGGGACAAACC
  AGGCAGCTATATCTTCCGGCCAAGCTGTACCCGCCTGGGGCAGTGGGCCATTGGATACGTGAGCTCCGAT
  GGAAGCATCCTGCAAACCATTCCTCTCAACAAACCTCTGCTGCAGGTGCTCCTGAAGGGACAAAAGGACG
  GCATCTTCCTCTTCCCTGATGGAAAGAAACACAACCCAGACCTGACTGAGCTCTGCCGGGTAGAACCCTA
  CCAACGCATCCAAGTGTCAGAGGAACAACTGCTGCTCTACCAAGCCATGAACTCCACGTTCCAGCTGTGC
  AAGATCTGCGCAGAGAGGGACAAGGACGTGAGGATCGAGCCCTGTGGGCATCTTCTCTGCAGCTGCTGCC
  TGGCTGCCTGGCAGGACTCAGACAGCCAGACCTGTCCCTTCTGCCGTTGTGAGATCAAAGGTCGAGAGGC
  CGTGAGTATCTGTCAGGCACAGGAGAGGCCAACGGAGGTCAGGACTGCTGCAGATGGCTCAAGAGACAAC
  TGTCACCAGGAGGCTGCTGAGCAGAAACTGGGGCCGGTGATTCCCTCTGCTCCCTCACTACTCCCTGAAG
  ATCAGTTCCCTCAGGGACCCCAAGACAAAGGCTGGCTGACACTGGCACCACTCGCCCTGCCCAGGCTCCG
  ACCTCCACTTCCTCTCCCCAAAATGGCCTCGGTTCTGTGGGAAGTCACCTCCAGGCCCCGGGCCAGGGAG
  GAGGCCACAGAAAGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220739 representing NM_023224
  Red=Cloning site Green=Tags(s)
  
  MAAAAAPRGWQRGEPRALSRAVKLLQRLEEQCRDPRMVTGPPSLRDLLPRTAQLLGEVAKARREAREDPE
  GPGGADDFLAIYLANLEVKGRQVAELLPPRGKKDVNQDVFREGSRFRRQLAKLALIFSHMHAELSALFPA
  GKYCGHLYQLTKGSAHIFWRQNCGVRCVLPWAEFQSLLCACHPVEPGPTMQALRSTLDLTCNGHVSVFEF
  DVFTRLFQPWPTLLRNWQLLAVNHPGYMAFLTYDEVQTRLQAYRDKPGSYIFRPSCTRLGQWAIGYVSSD
  GSILQTIPLNKPLLQVLLKGQKDGIFLFPDGKKHNPDLTELCRVEPYQRIQVSEEQLLLYQAMNSTFQLC
  KICAERDKDVRIEPCGHLLCSCCLAAWQDSDSQTCPFCRCEIKGREAVSICQAQERPTEVRTAADGSRDN
  CHQEAAEQKLGPVIPSAPSLLPEDQFPQGPQDKGWLTLAPLALPRLRPPLPLPKMASVLWEVTSRPRARE
  EATESS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023224
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023224.5, NP_075713.3
• RefSeq Size: 1677 bp
• RefSeq ORF: 1491 bp
• Locus ID: 80794
• UniProt ID: Q80XL1
• Cytogenetics: 7 A3
• Gene Summary: Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-424', has the highest ubiquitin ligase activity among CBL family proteins. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.[UniProtKB/Swiss-Prot Function]