Dsg4 (NM_181564) Mouse Tagged ORF Clone

CAT#: MG220643

Dsg4 (tGFP-tagged) - Mouse desmoglein 4 (Dsg4), (10ug)

Product Images

Circular map for MG220643

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dsg4
• Synonyms: CDHF13; l; lah
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220643 representing NM_181564
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGCTTCTCTTCAGAAATATTTGCCTTCTGATCTTATTTATGGTGGTACTGGGAGTAAACAGTG
  AATTTATCGTTGAGGTAAAAGAATTAGACATTGAAAATGGCACAACAACCTGGCAGACTGTCAGAAGACA
  AAAGCGGGAGTGGATCAAGTTTGCGGCAGCCTGTCGGGAGGGCGAGGACAACTCCAAGAGGAATCCAATT
  GCCAGAATTAGATCAGACTGTGAAGTAAGCCAGAGGATAACCTATCGAATCTCTGGAGCGGGGATTGATC
  GGCCACCTTACGGAGTGTTCACCATCAACCCTCGGACTGGAGAAATCAATATTACCTCAGTGGTGGACAG
  AGAGATAACCCCACTGTTCCTGATCCATTGCCGAGCTCTCAACTCCCGAGGAGAAGACTTGGAGAGACCA
  CTGGAGCTCAGAGTGAAAGTCATGGATGTGAACGACAACCCCCCAGTCTTCACTCAGAATGTGTACACAG
  CCAACATTGAAGAGAACAGCGACGCCAATGCACTGGTGGTAAAGTTAAGTGCCACAGATGCAGATGAAGA
  CAATCACTTGAATTCTAAAATTGCATACAAGATCATCTCCCAGGAGCCTGCTGGTGCACCCATGTTCATG
  GTGAACAGGTACACTGGAGAAGTCCGCACGATGTCCAATTTCCTTGACAGAGAGCAACACAGTATGTACA
  ATCTACTGGTGAGGGGCTCAGATCGGGATGGAGCCACAGATGGGCTGTCCTCTGAATGTGACTGCAGAAT
  CAAGATTTTGGATGTCAATGATAATTTCCCCATCTTGGAGAAGACCTCATACTCAGCAAGTATTGAAGAA
  AATTGTTTGAGCTCAGAACTGATACGACTACAAGCAATCGATCTGGATGAAGAAGGCACTGATAACTGGT
  TGGCACAATACTCCATCCTCTCTGGCAATGATGGGAACTGGTTTGAAATCCAAACAGATCCAAAAACCAA
  TGAAGGCATTTTGAAAGTGGTCAAGATGCTGGATTATGAACAAGAACCTAACATTTACCTCAGCATTGGA
  GTTAGAAACCAAGCTGAGTTTCACCACTCAGTAGCATCTCAATTCCAAATGCACTCTACCCCTGTGAGAA
  TCCAGGTTGTTAACGTGCGGGAAGGACCGACCTTTAGCCCAAGTTCCATGACATTTAGTCTACGAGGAGG
  AATGAGAGGAGCCTCCTTAATGAACTATGTGCTTGGCACATACACAGCCATAGATATGGACACAGGAAAC
  CCTGCAACCAATGTCAGGTATGTCATAGGGCATGATGCAGGCAGCTGGTTAAAAGTTGATTCAAGGACTG
  GTGAGATACAGTTTTCTAGGGAATTTGACATGAAGTCAAAATATATTACGGATGGGATATATGCAGCACA
  GATCCTGGCTATAGATGATGGCTCTGGGAGAACAGCTACAGGAACCATATGCATTGAGATTCCTGATGCC
  AACGATTACTGTCCCGTCATTTATGCTGAAAGCAGGAGTGTCTGCACCCATGCTTCGTCTGTCAGGATCT
  ATGTCAACGATCATTCTTTCGGGTCTCCCTTTACCTTTTGTGTAGTTGATGAGTCACCAGACATAGCTAA
  TATATGGGATATCAGGTCCATCAATGGAACCTCTGCAATCCTTATGACTGAGCAGACTTTGTCTCCAGGA
  CCCTACCAAATCCCCATCCTCGTGAAGGACAGTCACAACCGAGCATGTGAATTGCCACAGACAGTGCTGT
  TAGATGCCTGCTTTTGTGATGACCACCATGTGTGCTTGCACTCCAGTACCACGGGCATCTACACTGGAGA
  CATCACCTGGGTGACTGATGACATGTATGGGACTGTCACTGATGATGGAGTTAGGCAGTCAAACGTCGGA
  CTTGGCCCAGCAGGGATTGGCATGATTATTTTGGGGCTCTTGCTACTGCTCTTGTCACCCCTTTTACTGC
  TCATGTGTTGCTGCAAGCGAAGACAGCCAGAAGGCCTGGGGACAAGGTTTGCCCCTGTGCCTGAGGGAGG
  CGAAGGAGTGATGCAGCCCTGGAGAATAGAGGGGGCTCATCCAGAAGACAGGGATGTATCAAATATTTGT
  GTACCCATGACAGCCTCTAATACCCAAGATCGCATTGATTCATCTGAAATCTACACCAATACTTACGCTG
  GTGGAGGAACAGTGGAAGGGGGCGTGTCAGGAGTGGAGCTCAATACAGGTGTTGGGACAGCCACAGGCAT
  GGTGGCTGCAGGAGCAACAGGGACCCTAAGGAAGAGGAGTTCAACTATAGGGACTCTTCGGGAATACCAA
  GACACTGGCATGAACATGGCCTTCCTGGACAGCTACTTCTCCGAGAAAGCGTATGCATATGCAGATGAAG
  ATGAAGGCCGACCAGCCAATGACTGCCTGCTCATTTATGATCACGAAGGAGCTGGGTCACCCGTGGGCTC
  CATTGGCTGCTGCAGTTGGATTGTGGATGACTTAGACGAAAGCTACATAGAGACTTTGGATCCAAAGTTC
  AGGACTCTAGCTGAAATCTGCTTGGACACAGAGATTGAACCCTTCCCTTCACACCAGGCCTGTATACCAA
  TCAGCACTGACCTCCCTCTGCTGGGGCCTAATTACTTTGTCAATGAGTCTTCAGGAATGACTCTCTCAGA
  GGCTGAATTCCAAGCAGAAATGGCAGCAGCCTCTGAACCCATGATTCATGGGGACATCATAGTGACTGAG
  ACCTACACTACTTCTGACCCATGTGTCCAACCCACAACGATTGTCTTTGACTCTCAGATTCCACCAAACG
  TAGTCGTGACCGAAACCGTAATGGCACCTGTCTACGATGTTCAAGGGAACATTTGTGTGCCTGCTGAGAT
  AGCCAACACACACAATGTATACTATGCTGAGAGAGTGGTGGCTAGCCCTGGTATACCTGACATGGGCAAC
  AGTAACATTAGTGATGCATGTATAGGACCAGTGATGAGCGGCGGTATTTTGGTAGGGCCAGAAATTCAAG
  TGACACAAATGATGAGCCCAGACATTCACATAAGCCAAACCACAGGCTCCACCTCCCCAATGACGTCTCA
  ACACCGAGTAACCAGATACAGCAATATGCACTACTCCCGACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220643 representing NM_181564
  Red=Cloning site Green=Tags(s)
  
  MDWLLFRNICLLILFMVVLGVNSEFIVEVKELDIENGTTTWQTVRRQKREWIKFAAACREGEDNSKRNPI
  ARIRSDCEVSQRITYRISGAGIDRPPYGVFTINPRTGEINITSVVDREITPLFLIHCRALNSRGEDLERP
  LELRVKVMDVNDNPPVFTQNVYTANIEENSDANALVVKLSATDADEDNHLNSKIAYKIISQEPAGAPMFM
  VNRYTGEVRTMSNFLDREQHSMYNLLVRGSDRDGATDGLSSECDCRIKILDVNDNFPILEKTSYSASIEE
  NCLSSELIRLQAIDLDEEGTDNWLAQYSILSGNDGNWFEIQTDPKTNEGILKVVKMLDYEQEPNIYLSIG
  VRNQAEFHHSVASQFQMHSTPVRIQVVNVREGPTFSPSSMTFSLRGGMRGASLMNYVLGTYTAIDMDTGN
  PATNVRYVIGHDAGSWLKVDSRTGEIQFSREFDMKSKYITDGIYAAQILAIDDGSGRTATGTICIEIPDA
  NDYCPVIYAESRSVCTHASSVRIYVNDHSFGSPFTFCVVDESPDIANIWDIRSINGTSAILMTEQTLSPG
  PYQIPILVKDSHNRACELPQTVLLDACFCDDHHVCLHSSTTGIYTGDITWVTDDMYGTVTDDGVRQSNVG
  LGPAGIGMIILGLLLLLLSPLLLLMCCCKRRQPEGLGTRFAPVPEGGEGVMQPWRIEGAHPEDRDVSNIC
  VPMTASNTQDRIDSSEIYTNTYAGGGTVEGGVSGVELNTGVGTATGMVAAGATGTLRKRSSTIGTLREYQ
  DTGMNMAFLDSYFSEKAYAYADEDEGRPANDCLLIYDHEGAGSPVGSIGCCSWIVDDLDESYIETLDPKF
  RTLAEICLDTEIEPFPSHQACIPISTDLPLLGPNYFVNESSGMTLSEAEFQAEMAAASEPMIHGDIIVTE
  TYTTSDPCVQPTTIVFDSQIPPNVVVTETVMAPVYDVQGNICVPAEIANTHNVYYAERVVASPGIPDMGN
  SNISDACIGPVMSGGILVGPEIQVTQMMSPDIHISQTTGSTSPMTSQHRVTRYSNMHYSRQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181564
• ORF Size: 3123 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181564.2, NP_853543.1
• RefSeq Size: 3478 bp
• RefSeq ORF: 3126 bp
• Locus ID: 16769
• UniProt ID: Q7TMD7
• Cytogenetics: 18 11.35 cM
• Gene Summary: This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. This gene is expressed in the suprabasal epidermis and hair follicle. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Certain mutations in this gene are responsible for the lanceolate hair phenotype in mice. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. [provided by RefSeq, Feb 2016]