Ttc19 (NM_028360) Mouse Tagged ORF Clone

CAT#: MG220513

Ttc19 (tGFP-tagged) - Mouse tetratricopeptide repeat domain 19 (Ttc19) transcript variant 1, (10ug)

Product Images

Circular map for MG220513

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ttc19
• Synonyms: 2010204O13Rik; 2810460C24Rik; AI505442
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220513 representing NM_028360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGTTGCTTCGCTGGCGTCTGGGTCGAACCCTGCTGCGGGCCGCGGGCCGGCGGTGTGGGGGTT
  GTACGGCGCGCCTGCTGCCGGAGCGGACGGGCGACGCGGGGACGGGCGCGGAGCGGCTGCGGACCCGAGG
  CGCGCCGGCGCGGGGGCACGGGGTGCTGCCGCTGTTGGCAGCTCTCGCCTGGTTCTCGCGACCCGCGGCG
  ACGGCCGAGCAGCCGGGAGAGGACGCGTCAGACGAGGCGGAGGCCGAGATCATCCAGCTGCTGAAGCAAG
  CCAAGTTGAGCATCATGAAAGACGAGCCAGAAGCGGCCGAGCTGATTTTGCATGACGCTCTTCGCCTTGC
  GTATGAGAGTGACAACAGGAAGGCCATCACGTACACTTATGACCTGATGGCCAACTTAGCATTTATACGA
  GGGCAGCTTGAAAATGCAGAACAGCTTTTTAAAGCAACGATGAGTTATCTGCTTGGAGGAGGCATGAAGC
  AGGAAGACAATGCAATAATTGAAATCTCTCTGAAACTGGCCAACATCTATGCTGCTCAGAATAAACAGGA
  ATTTGCCCTTGCTGGCTATGAATTCTGCATTTCAACTCTAGAGGGGAAAATTGAAAGAGAAAAGGAATTA
  GCAGAAGATATTATGTCAGAAGAAACAGCCAATACTTACCTCCTCCTTGGTATGTGCTTAGACTCTTGTG
  CACGCTATCTGCTATTCTCCAAGCAGCTATCACAGGCACAAAGGATGTATGAAAAAGCCCTGCAGATTTG
  TCAAGAAATACAAGGAGAAAGACACCCACAGACCATTGTGCTGATGAGTGACTTGGCTACTACTCTGGAT
  GCACAGGGCCACTTTGATGATGCCTACATTTATATGCAGAGGGCGTCAGATCTGGCAAGAGAGATAAATC
  ACCCTGAGCTGCACATGGTACTCAGCAATCTGGCTGCCATTTTGATACACAGAGAGCGATACACACAAGC
  AAAGGAGATCTACCAGGAAGCGCTGAAGCGAGCAGAGCTGAAAAGAGATGAGGTTTCTGTTCAGCACATC
  AGGGAAGAATTGGCTGAACTGTCAAGAAAAAGTAGACGTTTGACT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220513 representing NM_028360
  Red=Cloning site Green=Tags(s)
  
  MFRLLRWRLGRTLLRAAGRRCGGCTARLLPERTGDAGTGAERLRTRGAPARGHGVLPLLAALAWFSRPAA
  TAEQPGEDASDEAEAEIIQLLKQAKLSIMKDEPEAAELILHDALRLAYESDNRKAITYTYDLMANLAFIR
  GQLENAEQLFKATMSYLLGGGMKQEDNAIIEISLKLANIYAAQNKQEFALAGYEFCISTLEGKIEREKEL
  AEDIMSEETANTYLLLGMCLDSCARYLLFSKQLSQAQRMYEKALQICQEIQGERHPQTIVLMSDLATTLD
  AQGHFDDAYIYMQRASDLAREINHPELHMVLSNLAAILIHRERYTQAKEIYQEALKRAELKRDEVSVQHI
  REELAELSRKSRRLT
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028360
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028360.2, NP_082636.3
• RefSeq Size: 3419 bp
• RefSeq ORF: 1098 bp
• Locus ID: 72795
• UniProt ID: Q8CC21
• Cytogenetics: 11 B2
• Gene Summary: Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed:21278747, PubMed:28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation into the complex III and whose presence is detrimental for its catalytic activity (PubMed:28673544).[UniProtKB/Swiss-Prot Function]