Nipal3 (NM_028995) Mouse Tagged ORF Clone

CAT#: MG220361

Nipal3 (tGFP-tagged) - Mouse NIPA-like domain containing 3 (Nipal3), (10ug)

Product Images

Circular map for MG220361

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nipal3
• Synonyms: 9130020G22Rik; AI480660; Npal3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220361 representing NM_028995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGAGCCCACAGTGCAGGTCTGCAGCTGCAGCCACTCCCACCCACCAGCGGCGCCACCTCCACGA
  GCCTGAGCAGCAGCGAGGGCTCCTTCTCCTACAAGGAAAACTTGATCGGAGCACTCTTGGCCATTTTTGG
  GCACCTGGTGGTCAGCATTGCCCCTAACCTGCAGAAATACTGCCACATCCGCCTAGCAGGCTCCAAGGAC
  CCACGGGCCTACTTCAAGACCAAGACATGGTGGCTGGGGCTGCTCCTGCTGCTACTGGGGGAACTGGGCG
  TGTTCGCCTCCTACGCCTTTGCTCCACTGTCGCTCATCGTGCCCCTCAGTGCCGTCTCCGTGATAGCAAG
  CGCCATCATCGGAATCATATTCATCAAGGAAAAATGGAAACCTAAGGACTTTGTGAGACGCTATGTCTTG
  TCCTTTGTTGGCTGCGGCTTGGCCATCGTGGGCACCTACCTGCTAGTGACATTTGCACCCAACAGTCATG
  AGAAGATGACAGGCGAGAACATCGCCAGGCACCTTGTGAGCTGGCCGTTTCTCCTGTACATGCTGGTGGC
  GATCGTCCTGTTCTGCCTGCTGCTCTACTTCTACAAGGAGAGGAACGCCAACAGCATCGTTGTGATTCTC
  CTCCTGGTGGCCCTGCTTGGTTCCATGACAGTGGTGACGGTCAAGGCCGTGTCAGGGATGCTGGTGCTGT
  CCATCCAAGGCAACCTGCAGCTGGACTACCCCATCTTCTACGTGATGTTTGTGTGCATGGTGGCCACCGC
  CATCTATCAGGCCACGTTTCTGAGTGAAGCCTCCCAGATCTATGACTCCTCCTTGATTGCCAGCGTTGGC
  TACATCCTGTCCACAACAGCGGCCATCACAGCAGGTGCGATCTTCTACCTGGACTTCCTTGGGGAGGAAG
  CTCTGCACATCTGTATGTTTGCACTGGGGTGCCTCATCGCCTTCTTGGGCGTGTTCCTAATCACTCGAAA
  CAGAAAGAAGGCCATTCCGTTCGAGCCCTATATTTCCATGGACGCCATGCCAGGTATGCAGGACATGCAT
  GCCAAAGGGACAACGGTCCAGCCTGACCTCAAAGCTTCTTTTTCCTACGGAGCCTTGGAAAGCAACGACA
  GCATTTCTGAGCTCTACACCCCGGCCACACTGCCCGTCATGCAGGAGGAGCACAGCTCCAGGAGCACCCC
  TGGGGTTCCCTACCGCGTCCTGGAGCACACCAAGAAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220361 representing NM_028995
  Red=Cloning site Green=Tags(s)
  
  MDGAHSAGLQLQPLPPTSGATSTSLSSSEGSFSYKENLIGALLAIFGHLVVSIAPNLQKYCHIRLAGSKD
  PRAYFKTKTWWLGLLLLLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKPKDFVRRYVL
  SFVGCGLAIVGTYLLVTFAPNSHEKMTGENIARHLVSWPFLLYMLVAIVLFCLLLYFYKERNANSIVVIL
  LLVALLGSMTVVTVKAVSGMLVLSIQGNLQLDYPIFYVMFVCMVATAIYQATFLSEASQIYDSSLIASVG
  YILSTTAAITAGAIFYLDFLGEEALHICMFALGCLIAFLGVFLITRNRKKAIPFEPYISMDAMPGMQDMH
  AKGTTVQPDLKASFSYGALESNDSISELYTPATLPVMQEEHSSRSTPGVPYRVLEHTKKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028995
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028995.3, NP_083271.1
• RefSeq Size: 5025 bp
• RefSeq ORF: 1233 bp
• Locus ID: 74552
• UniProt ID: Q8BGN5
• Cytogenetics: 4 D3