Snx9 (NM_025664) Mouse Tagged ORF Clone

CAT#: MG220304

Snx9 (tGFP-tagged) - Mouse sorting nexin 9 (Snx9), (10ug)

Product Images

Circular map for MG220304

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snx9
• Synonyms: 2700073N08Rik; SDP1; SH3PX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220304 representing NM_025664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCAAGGCTCGGGTCATGTACGACTTTGCTGCTGAGCCTGGAAACAATGAGCTGACCGTTACTG
  AAGGAGAAATCATCACAGTTACAAACCCGAATGTTGGTGGAGGATGGCTGGAAGGAAAGAACAACAAAGG
  AGAGCAGGGGCTTGTTCCTACTGACTACGTGGAAATTTTACCAAACGATGGAAAAGATCCGTTTTCTTGT
  GGGAATTCAGTAGCCGACCAAGCTTTCCTCGATTCCCTGACAGCTAGCACAGCTCAAACCAACTCATCTT
  CTGCCAACAGCAATAACCAGGTTGGTGGTGGCAATGACCCCTGGACAGCCTGGAATGCTCCCAAACCTGG
  GAACTGGGACAGCTCCGATGCCTGGGGCTCCAGGACAGACGGAACTAGCGCTCAGAGGAACAGCAGCGCC
  AACAACTGGGACACGGGCTTTGGCCACCCACAGGCCTACCAAGGACCAGCAACTGGTGATGATGACGAAT
  GGGATGAAGACTGGGACGACCCCAAGTCCTCCTCACCTTACTTCAAGGATTCAGAACCAGCTGAAGCAGG
  GGGCATCCAGCGGGGAAACAGTCGTGCGGGTGCCTCCTCCATGAAGCTGCCCCTTAACAAATTTCCTGGA
  TTTGCAAAACCTGGCATGGAGCAGTACTTGTTGGCCAAGCAGCTAGCAAAACCCAAAGAGAAAATCGCCA
  TCATCGTTGGAGATTATGGCCCTATGTGGGTTTATCCTACCTCTACGTTTGACTGTGTGGTAGCTGATCC
  CCGGAAAGGTTCCAAAATGTACGGTCTGAAGAGCTACATTGAGTATCAGCTAACACCCACTAACACTAAC
  CGATCTGTAAACCACAGATATAAGCACTTTGATTGGTTATATGAGCGTCTCCTGGTTAAGTTTGGGTCTG
  CCATTCCGATCCCTTCTCTTCCAGACAAGCAGGTCACAGGTCGCTTTGAAGAGGAATTTATCAAAATGCG
  GATGGAGCGGCTCCAGGCCTGGATGACCAGGATGTGCCGGCACCCGGTGGTGTCAGAAAGTGAAGTTTTC
  CAGCAGTTCCTGAATTTCCGAGATGAGAAGGAATGGAAGACTGGAAAGAGGAAGGCTGAGAAAGACGAAC
  TGGTGGGAGTGATGATATTTTCCACCATGGAACCAGAGGCTCCTGACTTGGATTTGATAGAAATAGAACA
  GAAGTGTGACGCTGTGGGGAAGTTCACCAAGGCCATGGATGACGGCGTGAAGGAGCTGCTGACGGTGGGG
  CAGGAGCACTGGAAGCGCTGCACAGGGCCCTTACCCAAGGAGTATCAGAAGATAGGGAAGGCTCTGCAGA
  GCTTAGCGGCAGTGTTCAGCTCCAGCGGTTATCAAGGAGAGACCGACCTTAACGATGCAATCACAGAAGC
  TGGGAAGACGTATGAGGAGATCGCCAGCCTTGTGGCAGAGCAGCCAAAGAAGGACCTCCACTTCCTGATG
  GAGTGCAACCATGAGTACAAAGGCTTTCTCGGCTGCTTCCCGGACATCATTGGTGCCCACAAGGGAGCAA
  TAGAAAAAGTGAAAGAAAGTGATAAGCTGGTTGCAACTAGTAAAATTACTCCCCAAGACAAGCAGACCAT
  GGTGAAGAGAGTTGGCACCATGTCTTACGCTCTGCAAGCTGAGATGAATCACTTTCACAGTAACCGGATC
  TATGATTACAACAGTGTCATCCGCCTCTACCTGGAGCAGCAAGTGCAGTTCTATGAAACAATCGCAGAAA
  AGCTGAGGCAGGCCCTCAGCCGCTTCCCGGTTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220304 representing NM_025664
  Red=Cloning site Green=Tags(s)
  
  MATKARVMYDFAAEPGNNELTVTEGEIITVTNPNVGGGWLEGKNNKGEQGLVPTDYVEILPNDGKDPFSC
  GNSVADQAFLDSLTASTAQTNSSSANSNNQVGGGNDPWTAWNAPKPGNWDSSDAWGSRTDGTSAQRNSSA
  NNWDTGFGHPQAYQGPATGDDDEWDEDWDDPKSSSPYFKDSEPAEAGGIQRGNSRAGASSMKLPLNKFPG
  FAKPGMEQYLLAKQLAKPKEKIAIIVGDYGPMWVYPTSTFDCVVADPRKGSKMYGLKSYIEYQLTPTNTN
  RSVNHRYKHFDWLYERLLVKFGSAIPIPSLPDKQVTGRFEEEFIKMRMERLQAWMTRMCRHPVVSESEVF
  QQFLNFRDEKEWKTGKRKAEKDELVGVMIFSTMEPEAPDLDLIEIEQKCDAVGKFTKAMDDGVKELLTVG
  QEHWKRCTGPLPKEYQKIGKALQSLAAVFSSSGYQGETDLNDAITEAGKTYEEIASLVAEQPKKDLHFLM
  ECNHEYKGFLGCFPDIIGAHKGAIEKVKESDKLVATSKITPQDKQTMVKRVGTMSYALQAEMNHFHSNRI
  YDYNSVIRLYLEQQVQFYETIAEKLRQALSRFPVM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025664
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025664.6
• RefSeq Size: 2078 bp
• RefSeq ORF: 1788 bp
• Locus ID: 66616
• UniProt ID: Q91VH2
• Cytogenetics: 17 3.51 cM
• Gene Summary: Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton (PubMed:23437151). Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate (By similarity).[UniProtKB/Swiss-Prot Function]