Slc35f4 (NM_029238) Mouse Tagged ORF Clone

CAT#: MG220189

Slc35f4 (tGFP-tagged) - Mouse solute carrier family 35 member F4 (Slc35f4), (10ug)

Product Images

Circular map for MG220189

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc35f4
• Synonyms: 4930550L21Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220189 representing NM_029238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCAAGGCGGCCCCCAACGGGGTGGCCACCATCGAGGACCGGATCCTGCGGATTACTGGCTACT
  ATGGCTATTACCCGGGTTACTCCAGCCAGAAAAGTACCACCAGATCATCAGTTACTCGATGCAAACCAGG
  ACCCAACTGCCCCAGTTCACACAGCAGCATCAGCAGGCAACTGTCCCCTCTGTCGGTCACGGAAGATTCT
  TCTGCTCCTATTCTTGAACTTCAGAGCCGAGGATCCTCTGGAGTTTGTGGTCGAAGAGTGGAGAGACAGA
  GCAGATCAGGGGATGATGGGACACAGACTCGTCCTGAGAGCAGCAGCCAAGAAAACGGACTCAAGGCTCG
  ATGCCTGTCCTGCACATCCATGGTTCTGAAGACCATCTGGGGACTCTTGATCATCTTGTCAGTGTCATCA
  TCTTGGGTTGGAACCACACAAATTGTAAAAATCACTTACAAGAATTTTTATTGTCCCTTTTTCATGACAT
  GGTTTTCAACAAACTGGAACATTATGTTTTTCCCAGTCTATTATTCCGGCCATCTAGCCACAGCTCAAGA
  AAAGCAGTCGCCAATAAAAAAATTCAGGGAATGCAGTCGGATTTTTGGTGAAGATGGCCTGACACTGAAG
  CTCTTTCTTAAAAGAACTGCTCCTTTTTCTATTCTGTGGACTTTGACAAATTACCTGTATTTGCTGGCTT
  TAAAGAAGCTGACGGCCACTGATGTCTCTGCTCTGTTCTGTTGCAACAAAGCCTTTGTCTTCTTGCTGTC
  CTGGATTGTGCTGAAGGACAGGTTCATGGGCGTGCGGATTGTCGCAGCAATAATGGCAATTACGGGCATT
  GTCATGATGGCGTATGCGGATAATTTCCACGCTGATTCCATCATCGGTGTGGCATTTGCCGTAGGCTCAG
  CCTCCACATCTGCACTGTATAAGGTTCTGTTTAAGATGTTTCTGGGCAGTGCCAACTTCGGTGAAGCTGC
  ACACTTTGTCTCCACCTTGGGGTTCTTCAATTTGATCTTTATCTCCTTCACTCCCATCATCCTGTATTTC
  ACCAAAGTGGAGCACTGGTCCTCCTTCGCAGCGCTGCCCTGGGGCTGCCTCTGTGGGATGGCGGGACTAT
  GGCTAGCATTCAACATCCTGGTCAATGTTGGAGTGGTACTGACATACCCAATCCTCATCTCCATTGGGAC
  AGTGCTCAGCGTCCCTGGAAACGCAGCTGTAGATCTCCTTAAGCAAGAAGTAATATTCAATGTAGTCCGC
  CTGGCTGCCACCATCATCATCTGTATCGGGTTTCTCCTGATGCTACTGCCGGAGGAGTGGGATGAAATTA
  CACTGAGATTTATCAACAGCCTGAAGGAGAAGAAGAGTGAGGAGCATGTGGAGGACCTGACAGATGTCAG
  CGTGCACCTGAGGAGCAGAAGCAGGGTCAACGGGACAGTGTCCATACCGCTGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220189 representing NM_029238
  Red=Cloning site Green=Tags(s)
  
  MDVKAAPNGVATIEDRILRITGYYGYYPGYSSQKSTTRSSVTRCKPGPNCPSSHSSISRQLSPLSVTEDS
  SAPILELQSRGSSGVCGRRVERQSRSGDDGTQTRPESSSQENGLKARCLSCTSMVLKTIWGLLIILSVSS
  SWVGTTQIVKITYKNFYCPFFMTWFSTNWNIMFFPVYYSGHLATAQEKQSPIKKFRECSRIFGEDGLTLK
  LFLKRTAPFSILWTLTNYLYLLALKKLTATDVSALFCCNKAFVFLLSWIVLKDRFMGVRIVAAIMAITGI
  VMMAYADNFHADSIIGVAFAVGSASTSALYKVLFKMFLGSANFGEAAHFVSTLGFFNLIFISFTPIILYF
  TKVEHWSSFAALPWGCLCGMAGLWLAFNILVNVGVVLTYPILISIGTVLSVPGNAAVDLLKQEVIFNVVR
  LAATIIICIGFLLMLLPEEWDEITLRFINSLKEKKSEEHVEDLTDVSVHLRSRSRVNGTVSIPLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029238
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029238.2, NP_083514.1
• RefSeq Size: 1881 bp
• RefSeq ORF: 1458 bp
• Locus ID: 75288
• UniProt ID: Q8BZK4
• Cytogenetics: 14 C1
• Gene Summary: Putative solute transporter.[UniProtKB/Swiss-Prot Function]